USER friendly DNA engineering and cloning method by uracil excision (Q42779885)

7 November 2017

USER friendly DNA engineering and cloning method by uracil excision (English)

1 reference

7 November 2017

Jurate Bitinaite

1

1 reference

7 November 2017

Michelle Rubino

2

1 reference

7 November 2017

Kamini Hingorani Varma

3

1 reference

7 November 2017

Ira Schildkraut

4

1 reference

7 November 2017

Romualdas Vaisvila

5

1 reference

7 November 2017

Rita Vaiskunaite

6

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7 November 2017

language of work or name

English

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publication date

6 March 2007

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7 November 2017

Nucleic Acids Research

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7 November 2017

35

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7 November 2017

6

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7 November 2017

1992-2002

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Advancing uracil-excision based cloning towards an ideal technique for cloning PCR fragments.

7 November 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1874603

Inducible control of transgene expression with ecdysone receptor: gene switches with high sensitivity, robust expression, and reduced size

27 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1874603

Site-specific DNA-nicking mutants of the heterodimeric restriction endonuclease R.BbvCI.

27 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1874603

Engineering strand-specific DNA nicking enzymes from the type IIS restriction endonucleases BsaI, BsmBI, and BsmAI.

27 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1874603

Mammalian 5-Formyluracil−DNA Glycosylase. 2. Role of SMUG1 Uracil−DNA Glycosylase in Repair of 5-Formyluracil and Other Oxidized and Deaminated Base Lesions

27 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1874603

Converting MlyI endonuclease into a nicking enzyme by changing its oligomerization state

27 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1874603

A read-ahead function in archaeal DNA polymerases detects promutagenic template-strand uracil.

27 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1874603

Cloning and assembly of PCR products using modified primers and DNA repair enzymes.

27 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1874603

Archaebacterial DNA polymerases tightly bind uracil-containing DNA.

27 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1874603

Generation of cohesive ends on PCR products by UDG-mediated excision of dU, and application for cloning into restriction digest-linearized vectors

27 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1874603

Substrate specificity of the Escherichia coli endonuclease III: excision of thymine- and cytosine-derived lesions in DNA produced by radiation-generated free radicals

27 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1874603

Use of the rep technique for allele replacement to construct new Escherichia coli hosts for maintenance of R6K gamma origin plasmids at different copy numbers

27 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1874603

Novel methods for cloning and engineering genes using the polymerase chain reaction

27 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1874603

An oligodeoxyribonucleotide-directed dual amber method for site-directed mutagenesis

27 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1874603

Rapid and efficient site-specific mutagenesis without phenotypic selection

27 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1874603

Structure of chloramphenicol acetyltransferase at 1.75-A resolution

27 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1874603

A general method of in vitro preparation and specific mutagenesis of DNA fragments: study of protein and DNA interactions

27 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1874603

The rapid generation of oligonucleotide-directed mutations at high frequency using phosphorothioate-modified DNA.

27 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1874603

Engineering hybrid genes without the use of restriction enzymes: gene splicing by overlap extension

27 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1874603

Site-directed mutagenesis by overlap extension using the polymerase chain reaction

27 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1874603

Rapid and efficient cloning of Alu-PCR products using uracil DNA glycosylase

27 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1874603

Site-directed mutagenesis of virtually any plasmid by eliminating a unique site

27 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1874603

Trans-complementation-dependent replication of a low molecular weight origin fragment from plasmid R6K

27 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1874603

DNA N-glycosidases: properties of uracil-DNA glycosidase from Escherichia coli

27 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1874603

Excision of 5'-terminal deoxyribose phosphate from damaged DNA is catalyzed by the Fpg protein of Escherichia coli

27 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1874603

Improved ecdysone receptor-based inducible gene regulation system

27 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1874603

Structural basis for uracil recognition by archaeal family B DNA polymerases

16 August 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1874603

An Escherichia coli vector to express and purify foreign proteins by fusion to and separation from maltose-binding protein

16 August 2018

1 reference

12 December 2020

A high efficiency method for site-directed mutagenesis with any plasmid

1 reference

12 December 2020

A novel method for site-directed mutagenesis using PCR and uracil DNA glycosylase

1 reference

12 December 2020

Assembly and cloning of coding sequences for neurotrophic factors directly from genomic DNA using polymerase chain reaction and uracil DNA glycosylase

1 reference

12 December 2020

Simultaneous introduction of multiple mutations using overlap extension PCR

1 reference

12 December 2020

Single-column purification of free recombinant proteins using a self-cleavable affinity tag derived from a protein splicing element

1 reference

12 December 2020

Characterization ofEscherichia coliEndonuclease VIII

1 reference

12 December 2020

Selection strategy for site-directed mutagenesis based on altered beta-lactamase specificity

1 reference

12 December 2020

Identifiers

DOI

10.1093/NAR/GKM041

1 reference

7 November 2017

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7 November 2017

PubMed publication ID

17341463

1 reference