Involvement of an active-site Zn2+ ligand in the catalytic mechanism of human glyoxalase I (Q24323845)

GOA release 2020-03-11

zinc ion binding

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GOA release 2020-03-11

author name string

M Ridderström

1

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A D Cameron

2

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T A Jones

3

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B Mannervik

Journal of Biological Chemistry

4

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language of work or name

English

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publication date

21 August 1998

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published in

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volume

273

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issue

34

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page(s)

21623-8

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cites work

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Glyoxalase I, a zinc metalloenzyme of mammals and yeast

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Probing the active site of glyoxalase I from human erythrocytes by use of the strong reversible inhibitor S-p-bromobenzylglutathione and metal substitutions

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Crystal structure of human glyoxalase I--evidence for gene duplication and 3D domain swapping

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Fluorescence and nuclear relaxation enhancement studies of the binding of glutathione derivatives to manganese-reconstituted glyoxalase I from human erythrocytes. A model for the catalytic mechanism of the enzyme involving a hydrated metal ion.

21 January 2018

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Design of two chimaeric human-rat class alpha glutathione transferases for probing the contribution of C-terminal segments of protein structure to the catalytic properties

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Optimized heterologous expression of the human zinc enzyme glyoxalase I

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AMoRe: an automated package for molecular replacement

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Improved methods for building protein models in electron density maps and the location of errors in these models

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Free R value: a novel statistical quantity for assessing the accuracy of crystal structures

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Refinement of macromolecular structures by the maximum-likelihood method

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Accurate bond and angle parameters for X-ray protein structure refinement

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The primary structure of monomeric yeast glyoxalase I indicates a gene duplication resulting in two similar segments homologous with the subunit of dimeric human glyoxalase I.

21 January 2018

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Molecular characterization of glyoxalase-I from a higher plant; upregulation by stress.

21 January 2018

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Identification of the structural gene for glyoxalase I from Saccharomyces cerevisiae

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The gene encoding glyoxalase I from Pseudomonas putida: cloning, overexpression, and sequence comparisons with human glyoxalase I.

21 January 2018

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Isolation and sequencing of a gene coding for glyoxalase I activity from Salmonella typhimurium and comparison with other glyoxalase I sequences

21 January 2018

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Mutagenesis of residue 157 in the active site of human glyoxalase I.

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A convenient quantitative synthesis of methylglyoxal for glyoxalase I assays

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7 January 2021

Kinetic parameters for the elimination reaction catalyzed by triosephosphate isomerase and an estimation of the reaction's physiological significance

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7 January 2021

Electron paramagnetic resonance study of the active site of copper-substituted human glyoxalase I

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7 January 2021

Evidence for a (triosephosphate isomerase-like) "catalytic loop" near the active site of glyoxalase I

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7 January 2021