Site-directed mutagenesis and the role of the oxyanion hole in subtilisin (Q24629501)

1

0 references

M W Pantoliano

2

0 references

S G Quill

3

0 references

H Y Hsiao

4

0 references

T Poulos

Proceedings of the National Academy of Sciences of the United States of America

5

0 references

language of work or name

English

0 references

publication date

June 1986

0 references

published in

0 references

volume

83

0 references

page(s)

3743-5

0 references

issue

11

0 references

cites work

Serine Proteases: Structure and Mechanism of Catalysis

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=323599

Crystal and molecular structure of chymotrypsin inhibitor 2 from barley seeds in complex with subtilisin Novo

7 April 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=323599

Cloning, sequencing, and secretion of Bacillusamyloliquefacienssubtillisin inBacillus subtilis

27 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=323599

Genes for alkaline protease and neutral protease from Bacillus amyloliquefaciens contain a large open reading frame between the regions coding for signal sequence and mature protein

27 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=323599

Engineering an enzyme by site-directed mutagenesis to be resistant to chemical oxidation

27 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=323599

Hydrogen bonding and biological specificity analysed by protein engineering

27 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=323599

Site-directed mutagenesis shows that tyrosine 248 of carboxypeptidase A does not play a crucial role in catalysis

27 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=323599

Substrate binding site in bovine chymotrypsin Aγ. Crystallographic study using peptide chloromethyl ketones as site-specific inhibitors

27 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=323599

X-ray crystallographic study of boronic acid adducts with subtilisin BPN' (Novo). A model for the catalytic transition state

30 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=323599

A sensitive new substrate for chymotrypsin

30 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=323599

-Chymotrypsin: What Can We Learn about Catalysis from X-Ray Diffraction?

30 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=323599

32 Oligonucleotide-directed mutagenesis of DNA fragments cloned into M13 vectors

30 May 2018

1 reference

12 December 2020

Restriction maps of plasmids pUB110 and pBD9

1 reference

12 December 2020

Structure of crystalline alpha-chymotrypsin. IV. The structure of indoleacryloyl-alpha-chyotrypsin and its relevance to the hydrolytic mechanism of the enzyme

1 reference

12 December 2020

Crystal structure studies and inhibition kinetics of tripeptide chloromethyl ketone inhibitors with Streptomyces griseus protease B

1 reference

12 December 2020

Subtilisin. Stereochemical mechanism involving transition-state stabilization

1 reference

12 December 2020

Polypeptide halomethyl ketones bind to serine proteases as analogs of the tetrahedral intermediate. X-ray crystallographic comparison of lysine- and phenylalanine-polypeptide chloromethyl ketone-inhibited subtilisin

1 reference

12 December 2020

An x-ray crystallographic study of the binding of peptide chloromethyl ketone inhibitors to subtilisin BPN'

1 reference

12 December 2020

Role of catalytic residues in the formation of a tetrahedral adduct in the acylation reaction of bovine beta-trypsin. A molecular orbital study

1 reference

12 December 2020

Directed mutagenesis of dihydrofolate reductase

1 reference

12 December 2020

Identifiers

DOI

10.1073/PNAS.83.11.3743

1 reference

1162309

ADS bibcode

1986PNAS...83.3743B

0 references

1162309

1 reference

1 reference

1 reference