Protein production and purification (Q24635875)

Europe PubMed Central

title

Protein production and purification (English)

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39

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Christian Cambillau

21

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Pär Nordlund

5

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Udo Oppermann

11

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Udo Heinemann

84

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Cheryl Arrowsmith

12

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Adelinda Yee

18

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Alexei Savchenko

17

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Steven C. Almo

57

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Johan Weigelt

6

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Susanne Gräslund

4

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Gaetano T. Montelione

79

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Aled Edwards

19

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Andrej Šali

63

Andrej Sali

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Susan Daenke

Susan Daenke

82

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Li-Wei Hung

Li-Wei Hung

28

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Chang-Yub Kim

Chang-Yub Kim

27

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Frank R. Collart

Frank Collart

40

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Opher Gileadi

Opher Gileadi

9

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Stephen K Burley

Stephen K Burley

48

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Joel L. Sussman

Joel L Sussman

35

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Liang Tong

Liang Tong

69

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Stefan Knapp

Stefan Knapp

10

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Thomas C. Terwilliger

Thomas C Terwilliger

26

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Shigeyuki Yokoyama

Shigeyuki Yokoyama

85

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David Ian Stuart

David I Stuart

80

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Ian Andrew Wilson

38

Ian A Wilson

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Sung-Hou Kim

23

Sung-Hou Kim

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Rosalind Kim

22

Rosalind Kim

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James E Bray

James Bray

8

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Andras Fiser

59

Andras Fiser

0 references

Li Zhao

66

Li Zhao

0 references

Rao Zihe

24

Zihe Rao

0 references

Jeffrey B Bonanno

58

Jeffrey B Bonanno

0 references

Raymond Hui

13

Raymond Hui

0 references

B. Martin Hallberg

7

B Martin Hallberg

0 references

Youngchang Kim

44

Youngchang Kim

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Irina Dementieva

41

Irina Dementieva

0 references

Scott A Lesley

37

Scott A Lesley

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author name string

Jinrong Ming

14

0 references

Sirano dhe-Paganon

15

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Hee-won Park

16

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Renaud Vincentelli

20

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Yunyu Shi

25

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Geoffrey S Waldo

29

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Yoav Peleg

30

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Shira Albeck

31

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Tamar Unger

32

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Orly Dym

33

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Jaime Prilusky

34

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Ray C Stevens

36

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Mark I Donnelly

42

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William H Eschenfeldt

43

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Lucy Stols

45

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Ruying Wu

46

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Min Zhou

47

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J Spencer Emtage

49

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J Michael Sauder

50

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Devon Thompson

51

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Kevin Bain

52

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John Luz

53

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Tarun Gheyi

54

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Fred Zhang

55

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Shane Atwell

56

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Sivasubramanian Swaminathan

60

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F William Studier

61

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Mark R Chance

62

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Thomas B Acton

64

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Rong Xiao

65

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Li Chung Ma

67

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John F Hunt

68

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Kellie Cunningham

70

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Masayori Inouye

71

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Stephen Anderson

72

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Heleema Janjua

73

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Ritu Shastry

74

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Chi Kent Ho

75

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Dongyan Wang

76

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Huang Wang

77

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Mei Jiang

78

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Raymond J Owens

81

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Anja Schütz

83

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Konrad Büssow

86

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Kristin C Gunsalus

An efficient mammalian cell-free translation system supplemented with translation factors

87

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language of work or name

English

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publication date

February 2008

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published in

Nature Methods

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volume

5

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issue

2

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page(s)

135-46

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cites work

1 reference

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Structural genomics of human proteins--target selection and generation of a public catalogue of expression clones

7 April 2017

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Structural genomics of the Thermotoga maritima proteome implemented in a high-throughput structure determination pipeline

7 April 2017

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DNA cloning using in vitro site-specific recombination

7 April 2017

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Production of Human Beta Interferon in Insect Cells Infected with a Baculovirus Expression Vector

7 April 2017

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A viral cleavage site cassette: identification of amino acid sequences required for tobacco etch virus polyprotein processing

7 April 2017

1 reference

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Novel protein fold discovered in the PabI family of restriction enzymes

7 April 2017

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Crystal structure of the rac activator, Asef, reveals its autoinhibitory mechanism

7 April 2017

1 reference

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Asymmetric DNA bending in the Cre-loxP site-specific recombination synapse

7 April 2017

1 reference

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Protein production by auto-induction in high density shaking cultures

7 April 2017

1 reference

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Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoter

7 April 2017

1 reference

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Use of T7 RNA polymerase to direct expression of cloned genes

7 April 2017

1 reference

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Intrinsic disorder in cell-signaling and cancer-associated proteins

7 April 2017

1 reference

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Heterologous expression, purification, and characterization of human triacylglycerol hydrolase

7 April 2017

1 reference

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Rapid screening for improved solubility of small human proteins produced as fusion proteins in Escherichia coli

7 April 2017

1 reference

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Escherichia coli maltose-binding protein is uncommonly effective at promoting the solubility of polypeptides to which it is fused

7 April 2017

1 reference

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Dynamic, structural, and regulatory aspects of lambda site-specific recombination

7 April 2017

1 reference

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The use of differential scanning fluorimetry to detect ligand interactions that promote protein stability

7 April 2017

1 reference

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A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding

7 April 2017

1 reference

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Combining the polymerase incomplete primer extension method for cloning and mutagenesis with microscreening to accelerate structural genomics efforts

7 April 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3178102

A time- and cost-efficient system for high-level protein production in mammalian cells

7 April 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3178102

3D-Jury: a simple approach to improve protein structure predictions

7 April 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3178102

Ligation-independent cloning of PCR products (LIC-PCR)

7 April 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3178102

The scientific impact of the Structural Genomics Consortium: a protein family and ligand-centered approach to medically-relevant human proteins

7 April 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3178102

Improved expression of kinases in Baculovirus-infected insect cells upon addition of specific kinase inhibitors to the culture helpful for structural studies

27 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3178102

Structural proteomics of the SARS coronavirus: a model response to emerging infectious diseases

27 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3178102

Vectors for co-expression of an unrestricted number of proteins

27 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3178102

Chemical screening methods to identify ligands that promote protein stability, protein crystallization, and structure determination

27 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3178102

First steps towards effective methods in exploiting high-throughput technologies for the determination of human protein structures of high biomedical value.

27 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3178102

The impact of protein characterization in structural proteomics.

27 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3178102

Implementation of semi-automated cloning and prokaryotic expression screening: the impact of SPINE.

27 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3178102

An efficient and generic strategy for producing soluble human proteins and domains in E. coli by screening construct libraries

27 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3178102

Strategies to maximize heterologous protein expression in Escherichia coli with minimal cost

27 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3178102

Cell-free expression systems for eukaryotic protein production

27 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3178102

Structural analysis and classification of native proteins from E. coli commonly co-purified by immobilised metal affinity chromatography

27 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3178102

Crystal structure of the SOCS2-elongin C-elongin B complex defines a prototypical SOCS box ubiquitin ligase

27 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3178102

An evaluation of in vitro protein-protein interaction techniques: assessing contaminating background proteins.

27 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3178102

Current strategies for the use of affinity tags and tag removal for the purification of recombinant proteins

27 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3178102

Structure and substrate specificity of the Pim-1 kinase

27 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3178102

High-throughput limited proteolysis/mass spectrometry for protein domain elucidation.

27 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3178102

RONN: the bio-basis function neural network technique applied to the detection of natively disordered regions in proteins

27 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3178102

Investigation of protein refolding using a fractional factorial screen: a study of reagent effects and interactions

27 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3178102

Making the most of affinity tags

27 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3178102

High-throughput automated refolding screening of inclusion bodies

27 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3178102

The DISOPRED server for the prediction of protein disorder

27 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3178102

High throughput protein production for functional proteomics

27 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3178102

Medium-scale structural genomics: strategies for protein expression and crystallization

27 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3178102

Facilities and methods for the high-throughput crystal structural analysis of human proteins.

27 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3178102

Protein expression systems for structural genomics and proteomics

27 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3178102

High-throughput screening for expression of heterologous proteins in the yeast Pichia pastoris.

27 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3178102

High-throughput proteomics: protein expression and purification in the postgenomic world.

27 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3178102

Protein NMR spectroscopy in structural genomics

27 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3178102

Overexpression of trigger factor prevents aggregation of recombinant proteins in Escherichia coli

27 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3178102

Controlling basal expression in an inducible T7 expression system by blocking the target T7 promoter with lac repressor

27 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3178102

Immobilized metal ion affinity chromatography

27 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3178102

Fusion proteins in biotechnology

27 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3178102

High-level production and optimization of monodispersity of 11beta-hydroxysteroid dehydrogenase type 1.

27 September 2017

1 reference

Crossref

https://api.crossref.org/works/10.1038%2FNMETH.F.202

The use of systematic N- and C-terminal deletions to promote production and structural studies of recombinant proteins.

21 January 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3178102

A generic protocol for the expression and purification of recombinant proteins in Escherichia coli using a combinatorial His6-maltose binding protein fusion tag.

28 November 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3178102

His-tag impact on structure.

28 November 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3178102

RIKEN aids international structural genomics efforts.

28 November 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3178102

Economical parallel protein expression screening and scale-up in Escherichia coli.

28 November 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3178102

Cell-free synthesis of zinc-binding proteins

28 November 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3178102

Plant Transformation by Agrobacterium tumefaciens: MODULATION OF SINGLE-STRANDED DNA-VirE2 COMPLEX ASSEMBLY BY VirE1

28 November 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3178102

Real-time observation of trigger factor function on translating ribosomes.

28 November 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3178102

The conformational quality of insoluble recombinant proteins is enhanced at low growth temperatures.

28 November 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3178102

Co-expression of protein complexes in prokaryotic and eukaryotic hosts: experimental procedures, database tracking and case studies.

28 November 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3178102

Recombinant protein expression and solubility screening in Escherichia coli: a comparative study.

28 November 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3178102

Eukaryotic expression: developments for structural proteomics.

28 November 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3178102

Strategies for protein coexpression in Escherichia coli

28 November 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3178102

High-throughput protein production for X-ray crystallography and use of size exclusion chromatography to validate or refute computational biological unit predictions

28 November 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3178102

Structural genomics of minimal organisms and protein fold space

28 November 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3178102

Three-dimensional structure determination of proteins related to human health in their functional context at The Israel Structural Proteomics Center (ISPC). This paper was presented at ICCBM10.

28 November 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3178102

Robotic cloning and Protein Production Platform of the Northeast Structural Genomics Consortium.

28 November 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3178102

Scalable high-throughput micro-expression device for recombinant proteins.

28 November 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3178102

Production of selenomethionine-labeled proteins in two-liter plastic bottles for structure determination

28 November 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3178102

Preparation of Escherichia coli cell extract for highly productive cell-free protein expression

28 November 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3178102

Structural genomics efforts at the Chinese Academy of Sciences and Peking University

28 November 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3178102

A micro-scale process for high-throughput expression of cDNAs in the yeast Saccharomyces cerevisiae.

28 November 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3178102

Cell-free translation reconstituted with purified components

28 November 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3178102

Generation of an AraC-araBAD promoter-regulated T7 expression system

28 November 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3178102

Protein production and crystallization at the joint center for structural genomics

28 November 2018

1 reference

12 December 2020

Solubility-enhancing proteins MBP and NusA play a passive role in the folding of their fusion partners

1 reference

12 December 2020

Construct for high-level expression and low misincorporation of lysine for arginine during expression of pET-encoded eukaryotic proteins in Escherichia coli

1 reference

12 December 2020

GroEL/GroES promote dissociation/reassociation cycles of a heterodimeric intermediate during alpha(2)beta(2) protein assembly. Iterative annealing at the quaternary structure level

1 reference

12 December 2020

High throughput protein production and crystallization at NYSGXRC.

1 reference

Crossref

https://api.crossref.org/works/10.1038%2FNMETH.F.202

7 January 2021

inferred from DOI database lookup

Identifiers

DOI

10.1038/NMETH.F.202

1 reference

Dimensions Publication ID

682935

1035202423

0 references

682935

1 reference

1 reference

1 reference