Measurements of the free luminal ER Ca(2+) concentration with targeted "cameleon" fluorescent proteins (Q28179581)

Technique for in situ measurement of calcium in intracellular inositol 1,4,5-trisphosphate-sensitive stores using the fluorescent indicator mag-fura-2.

Europe PubMed Central

title

Measurements of the free luminal ER Ca(2+) concentration with targeted "cameleon" fluorescent proteins (English)

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author

Maud Frieden

series ordinal

2

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Nicolas Demaurex

series ordinal

1

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language of work or name

English

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publication date

August 2003

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published in

Cell Calcium

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volume

34

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issue

2

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page(s)

109-19

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cites work

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Monitoring dynamic changes in free Ca2+ concentration in the endoplasmic reticulum of intact cells

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Aequorin targeted to the endoplasmic reticulum reveals heterogeneity in luminal Ca++ concentration and reports agonist- or IP3-induced release of Ca++.

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Ca2+-induced Ca2+ release in chromaffin cells seen from inside the ER with targeted aequorin

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Inositol 1,4,5-trisphosphate directs Ca(2+) flow between mitochondria and the Endoplasmic/Sarcoplasmic reticulum: a role in regulating cardiac autonomic Ca(2+) spiking

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