SUMO fusions and SUMO-specific protease for efficient expression and purification of proteins. (Q30342324)

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30 December 2019

title

SUMO fusions and SUMO-specific protease for efficient expression and purification of proteins (English)

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30 December 2019

5

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30 December 2019

author name string

Michael P Malakhov

1

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30 December 2019

Michael R Mattern

2

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30 December 2019

Oxana A Malakhova

3

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30 December 2019

Mark Drinker

4

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30 December 2019

Tauseef R Butt

6

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30 December 2019

publication date

1 January 2004

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Journal of Structural and Functional Genomics

30 December 2019

published in

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30 December 2019

volume

5

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30 December 2019

issue

1-2

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30 December 2019

page(s)

75-86

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Chemically synthesized ubiquitin extension proteins detect distinct catalytic capacities of deubiquitinating enzymes.

30 December 2019

cites work

1 reference

12 December 2020

A new general method for the biosynthesis of stable isotope-enriched peptides using a decahistidine-tagged ubiquitin fusion system: an application to the production of mastoparan-X uniformly enriched with 15N and 15N/13C.

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12 December 2020

The Ubiquitin Superfamily: Members, Features, and Phylogenies

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Increasing gene expression in yeast by fusion to ubiquitin.

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12 December 2020

Ubiquitin and its kin: how close are the family ties?

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12 December 2020

A new SUMO-1-specific protease, SUSP1, that is highly expressed in reproductive organs

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12 December 2020

Libraries of random-sequence polypeptides produced with high yield as carboxy-terminal fusions with ubiquitin

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12 December 2020

Regulation of ubiquitin-dependent processes by deubiquitinating enzymes

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12 December 2020

Protein expression using ubiquitin fusion and cleavage

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12 December 2020

The expression, refolding, and purification of the catalytic domain of human collagenase-3 (MMP-13)

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12 December 2020

Ubiquitin fusion augments the yield of cloned gene products in Escherichia coli

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12 December 2020

Reconstitution of the vitamin D-responsive osteocalcin transcription unit in Saccharomyces cerevisiae

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12 December 2020

Ulp1-SUMO crystal structure and genetic analysis reveal conserved interactions and a regulatory element essential for cell growth in yeast

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12 December 2020

A new 30-kDa ubiquitin-related SUMO-1 hydrolase from bovine brain

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12 December 2020

A new protease required for cell-cycle progression in yeast

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12 December 2020

Genetic design for facilitated production and recovery of recombinant proteins in Escherichia coli.

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12 December 2020

Integration of PCR fragments at any specific site within cloning vectors without the use of restriction enzymes and DNA ligase

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12 December 2020

The yeast ULP2 (SMT4) gene encodes a novel protease specific for the ubiquitin-like Smt3 protein

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12 December 2020

Structure determination of the small ubiquitin-related modifier SUMO-1

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12 December 2020

Ubiquitin-like proteins: new wines in new bottles

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12 December 2020

Functional heterogeneity of small ubiquitin-related protein modifiers SUMO-1 versus SUMO-2/3

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12 December 2020

Versatile protein tag, SUMO: its enzymology and biological function

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12 December 2020

Deubiquitinating enzymes: their diversity and emerging roles

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12 December 2020

An analysis of two refolding routes for a C-terminally truncated human collagenase-3 expressed in Escherichia coli

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12 December 2020

Rapid protein-folding assay using green fluorescent protein

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12 December 2020

Evidence for a three-state model of protein folding from kinetic analysis of ubiquitin variants with altered core residues

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12 December 2020

UBP43 (USP18) specifically removes ISG15 from conjugated proteins

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12 December 2020