Novel system for efficient isolation of Clostridium double-crossover allelic exchange mutants enabling markerless chromosomal gene deletions and DNA integration (Q30527850)

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English	Novel system for efficient isolation of Clostridium double-crossover allelic exchange mutants enabling markerless chromosomal gene deletions and DNA integration	scientific article

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scholarly article

1 reference

Europe PubMed Central

PMC publication ID

4 October 2017

Novel system for efficient isolation of Clostridium double-crossover allelic exchange mutants enabling markerless chromosomal gene deletions and DNA integration (English)

1 reference

Europe PubMed Central

PMC publication ID

4 October 2017

author name string

Mohab A Al-Hinai

1

1 reference

Europe PubMed Central

PMC publication ID

4 October 2017

Alan G Fast

2

1 reference

Europe PubMed Central

PMC publication ID

4 October 2017

Eleftherios T Papoutsakis

3

1 reference

Europe PubMed Central

PMC publication ID

4 October 2017

language of work or name

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publication date

14 September 2012

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Europe PubMed Central

PMC publication ID

4 October 2017

Applied and Environmental Microbiology

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Europe PubMed Central

PMC publication ID

4 October 2017

78

1 reference

Europe PubMed Central

PMC publication ID

4 October 2017

22

1 reference

Europe PubMed Central

PMC publication ID

4 October 2017

8112-8121

1 reference

Europe PubMed Central

PMC publication ID

4 October 2017

Integration of DNA into bacterial chromosomes from plasmids without a counter-selection marker

1 reference

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20 June 2018

Clostridia: the importance of their exceptional substrate and metabolite diversity for biofuel and biorefinery applications

1 reference

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20 June 2018

SpoIIE is necessary for asymmetric division, sporulation, and expression of sigmaF, sigmaE, and sigmaG but does not control solvent production in Clostridium acetobutylicum ATCC 824.

1 reference

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20 June 2018

Inactivation of σF in Clostridium acetobutylicum ATCC 824 blocks sporulation prior to asymmetric division and abolishes σE and σG protein expression but does not block solvent formation

1 reference

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20 June 2018

Multiple orphan histidine kinases interact directly with Spo0A to control the initiation of endospore formation in Clostridium acetobutylicum

1 reference

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20 June 2018

Inactivation of σE and σG in Clostridium acetobutylicum illuminates their roles in clostridial-cell-form biogenesis, granulose synthesis, solventogenesis, and spore morphogenesis

1 reference

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20 June 2018

Development and application of a method for counterselectable in-frame deletion in Clostridium perfringens

1 reference

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20 June 2018

Construction and characterization of a lactose-inducible promoter system for controlled gene expression in Clostridium perfringens

1 reference

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20 June 2018

Development of pyrF-based genetic system for targeted gene deletion in Clostridium thermocellum and creation of a pta mutant

1 reference

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20 June 2018

mRNA interferases, sequence-specific endoribonucleases from the toxin-antitoxin systems

1 reference

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20 June 2018

Engineering solventogenic clostridia

1 reference

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20 June 2018

Antisense-RNA-mediated decreased synthesis of small, acid-soluble spore proteins leads to decreased resistance of clostridium perfringens spores to moist heat and UV radiation

1 reference

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20 June 2018

Restriction for gene insertion within the Lactococcus lactis Ll.LtrB group II intron

1 reference

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20 June 2018

Construction and analysis of chromosomal Clostridium difficile mutants

1 reference

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20 June 2018

Use of targetrons to disrupt essential and nonessential genes in Staphylococcus aureus reveals temperature sensitivity of Ll.LtrB group II intron splicing

1 reference

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20 June 2018

Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection

1 reference

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20 June 2018

mazF, a novel counter-selectable marker for unmarked chromosomal manipulation in Bacillus subtilis.

1 reference

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20 June 2018

A conjugation-based system for genetic analysis of group II intron splicing in Lactococcus lactis

1 reference

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20 June 2018

Antisense RNA downregulation of coenzyme A transferase combined with alcohol-aldehyde dehydrogenase overexpression leads to predominantly alcohologenic Clostridium acetobutylicum fermentations

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3485963

20 June 2018

Targeted and random bacterial gene disruption using a group II intron (targetron) vector containing a retrotransposition-activated selectable marker

1 reference

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20 June 2018

Design of antisense RNA constructs for downregulation of the acetone formation pathway of Clostridium acetobutylicum

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3485963

20 June 2018

The genome sequence of Clostridium tetani, the causative agent of tetanus disease

1 reference

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20 June 2018

Bacterial group II introns and their association with mobile genetic elements

1 reference

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20 June 2018

Northern, morphological, and fermentation analysis of spo0A inactivation and overexpression in Clostridium acetobutylicum ATCC 824.

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3485963

20 June 2018

Development and characterization of a gene expression reporter system for Clostridium acetobutylicum ATCC 824

1 reference

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20 June 2018

Antisense RNA strategies for metabolic engineering of Clostridium acetobutylicum

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3485963

20 June 2018

Counterselectable markers: untapped tools for bacterial genetics and pathogenesis

1 reference

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20 June 2018

Use of time-lapse microscopy to visualize rapid movement of the replication origin region of the chromosome during the cell cycle in Bacillus subtilis

1 reference

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20 June 2018

Rolling-circle replication of bacterial plasmids.

1 reference

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Parameters controlling the rate of gene targeting frequency in the protozoan parasite Leishmania

1 reference

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20 June 2018

The genes for butanol and acetone formation in Clostridium acetobutylicum ATCC 824 reside on a large plasmid whose loss leads to degeneration of the strain

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3485963

20 June 2018

Different thermostabilities of FLP and Cre recombinases: implications for applied site-specific recombination

1 reference

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20 June 2018

In vivo methylation in Escherichia coli by the Bacillus subtilis phage phi 3T I methyltransferase to protect plasmids from restriction upon transformation of Clostridium acetobutylicum ATCC 824

1 reference

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20 June 2018

Expression of plasmid-encoded aad in Clostridium acetobutylicum M5 restores vigorous butanol production

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3485963

20 June 2018

Use of mutated FLP recognition target (FRT) sites for the exchange of expression cassettes at defined chromosomal loci

1 reference

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20 June 2018

Virulence studies on chromosomal alpha-toxin and theta-toxin mutants constructed by allelic exchange provide genetic evidence for the essential role of alpha-toxin in Clostridium perfringens-mediated gas gangrene

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3485963

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Chemotherapeutic tumour targeting using clostridial spores

1 reference

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Cleavage-dependent ligation by the FLP recombinase. Characterization of a mutant FLP protein with an alteration in a catalytic amino acid

1 reference

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The Minimum Amount of Homology Required for Homologous Recombination in Mammalian Cells

1 reference

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5-Fluoroorotic acid as a selective agent in yeast molecular genetics

1 reference

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1 reference

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20 June 2018

Gene-directed mutagenesis on the chromosome of Bacillus subtilis 168.

1 reference

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20 June 2018

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1 reference

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20 June 2018

Vector construction, transformation, and gene amplification in Clostridium acetobutylicum ATCC 824.

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3485963

20 June 2018

Precise manipulation of the Clostridium difficile chromosome reveals a lack of association between the tcdC genotype and toxin production.

1 reference

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26 November 2018

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1 reference

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26 November 2018

Metabolic engineering of the non-sporulating, non-solventogenic Clostridium acetobutylicum strain M5 to produce butanol without acetone demonstrate the robustness of the acid-formation pathways and the importance of the electron balance

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3485963

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Targeted gene disruption by use of a group II intron (targetron) vector in Clostridium acetobutylicum.

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3485963

26 November 2018

The α-toxin ofClostridium septicumis essential for virulence

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Genetic manipulation of acid formation pathways by gene inactivation in Clostridium acetobutylicum ATCC 824.

1 reference

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The ClosTron: a universal gene knock-out system for the genus Clostridium

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12 December 2020

based on heuristic

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Effects of ptb knockout on butyric acid fermentation by Clostridium tyrobutyricum

1 reference

https://pubmed.ncbi.nlm.nih.gov/22983967

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Expression of cloned homologous fermentative genes in Clostridium acetobutylicum ATCC 824

1 reference

https://pubmed.ncbi.nlm.nih.gov/22983967

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Determination of plasmid copy number and stability in Clostridium acetobutylicum ATCC 824

1 reference

https://pubmed.ncbi.nlm.nih.gov/22983967

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Inactivation of an aldehyde/alcohol dehydrogenase gene from Clostridium acetobutylicum ATCC 824

1 reference

https://pubmed.ncbi.nlm.nih.gov/22983967

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Identifiers

10.1128/AEM.02214-12

1 reference

Europe PubMed Central

PMC publication ID

4 October 2017

PMC publication ID

1 reference

Europe PubMed Central

PMC publication ID

4 October 2017

PubMed publication ID

1 reference

Europe PubMed Central

PMC publication ID

4 October 2017

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