Screening for interacting partners for G alpha i3 and RGS-GAIP using the two-hybrid system (Q30789644)

26 June 2017

title

Screening for interacting partners for G alpha i3 and RGS-GAIP using the two-hybrid system (English)

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26 June 2017

Luc De Vries

1

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26 June 2017

Marilyn Gist Farquhar

series ordinal

2

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26 June 2017

language of work or name

English

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publication date

1 January 2002

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26 June 2017

published in

Methods in Enzymology

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26 June 2017

volume

344

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26 June 2017

page(s)

657-673

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Membrane localization of the pertussis toxin-sensitive G-protein subunits alpha i-2 and alpha i-3 and expression of a metallothionein-alpha i-2 fusion gene in LLC-PK1 cells

26 June 2017

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7 January 2021

GAIP, a protein that specifically interacts with the trimeric G protein G alpha i3, is a member of a protein family with a highly conserved core domain

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The mammalian calcium-binding protein, nucleobindin (CALNUC), is a Golgi resident protein

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Activation of the ERK/MAPK pathway by an isoform of rap1GAP associated with G alpha(i)

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Interaction of the protein nucleobindin with G alpha i2, as revealed by the yeast two-hybrid system.

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7 January 2021

Identification and cDNA cloning of a novel human mosaic protein, LGN, based on interaction with G alpha i2

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7 January 2021

RGS10 is a selective activator of G alpha i GTPase activity

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7 January 2021

RGSZ1, a Gz-selective regulator of G protein signaling whose action is sensitive to the phosphorylation state of Gzalpha

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7 January 2021

Functional interaction between Galpha(z) and Rap1GAP suggests a novel form of cellular cross-talk

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Interaction of heterotrimeric G protein Galphao with Purkinje cell protein-2. Evidence for a novel nucleotide exchange factor

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Modulation of rap activity by direct interaction of Galpha(o) with Rap1 GTPase-activating protein.

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Diversity of G proteins in signal transduction

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GPR1 encodes a putative G protein-coupled receptor that associates with the Gpa2p Galpha subunit and functions in a Ras-independent pathway

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7 January 2021

GIPC, a PDZ domain containing protein, interacts specifically with the C terminus of RGS-GAIP

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7 January 2021

MIR16, a putative membrane glycerophosphodiester phosphodiesterase, interacts with RGS16

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7 January 2021

The two-hybrid system: a method to identify and clone genes for proteins that interact with a protein of interest

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Improved method for high efficiency transformation of intact yeast cells

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7 January 2021

GAIP is membrane-anchored by palmitoylation and interacts with the activated (GTP-bound) form of G alpha i subunits

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7 January 2021

Calnuc, an EF-hand Ca(2+) binding protein, specifically interacts with the C-terminal alpha5-helix of G(alpha)i3.

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Structural Basis for the Selectivity of the RGS Protein, GAIP, for G i Family Members: IDENTIFICATION OF A SINGLE AMINO ACID DETERMINANT FOR SELECTIVE INTERACTION OF G i SUBUNITS WITH GAIP

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7 January 2021

10.1016/S0076-6879(02)44747-7

Identifiers

DOI

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26 June 2017

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