Protein engineering by expressed protein ligation (Q30961015)

27 June 2017

title

Protein engineering by expressed protein ligation (English)

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27 June 2017

Blaschke UK

1

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27 June 2017

Silberstein J

series ordinal

2

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27 June 2017

Muir TW

series ordinal

3

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27 June 2017

language of work or name

English

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publication date

1 January 2000

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27 June 2017

published in

Methods in Enzymology

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27 June 2017

volume

328

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27 June 2017

page(s)

478-496

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Emerging approaches in the molecular design of receptor-selective peptide ligands: conformational, topographical and dynamic considerations

27 June 2017

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Peptide ligation and its application to protein engineering

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A designed peptide ligase for total synthesis of ribonuclease A with unnatural catalytic residues

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Expressed protein ligation, a novel method for studying protein-protein interactions in transcription

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Semisynthesis of cytotoxic proteins using a modified protein splicing element

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Synthesis of proteins by native chemical ligation

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A chemical approach to the construction of multimeric protein assemblies

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Protein synthesis by chemical ligation of unprotected peptides in aqueous solution

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Chemical ligation of unprotected peptides directly from a solid support

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Chemical Protein Synthesis by Solid Phase Ligation of Unprotected Peptide Segments

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Protein expression using ubiquitin fusion and cleavage

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Crystal structure of GyrA intein from Mycobacterium xenopi reveals structural basis of protein splicing

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Structural insights into the mechanism of intramolecular proteolysis

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Single-column purification of free recombinant proteins using a self-cleavable affinity tag derived from a protein splicing element

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Generation of a dual-labeled fluorescence biosensor for Crk-II phosphorylation using solid-phase expressed protein ligation

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The C-terminal carboxy group of T7 RNA polymerase ensures efficient magnesium ion-dependent catalysis.

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The cyclization and polymerization of bacterially expressed proteins using modified self-splicing inteins

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7 January 2021

Overexpression of recombinant proteins with a C-terminal thiocarboxylate: implications for protein semisynthesis and thiamin biosynthesis

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7 January 2021

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7 January 2021

Introduction of unnatural amino acids into proteins using expressed protein ligation

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Construction of protein analogues by site-specific condensation of unprotected fragments

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7 January 2021

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Identifiers

DOI

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27 June 2017

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