Recent advances in producing and selecting functional proteins by using cell-free translation (Q31972407)

5 July 2017

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title

Recent advances in producing and selecting functional proteins by using cell-free translation (English)

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5 July 2017

Jermutus L

1

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PubMed ID

9821285

5 July 2017

Ryabova LA

series ordinal

2

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PubMed ID

9821285

5 July 2017

Plückthun A

series ordinal

3

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5 July 2017

1 October 1998

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Current Opinion in Biotechnology

5 July 2017

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5 July 2017

9

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5 July 2017

534-548

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5 July 2017

5

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The potentials of the in vitro protein biosynthesis system.

5 July 2017

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2898%2980042-6

7 January 2021

In vitro synthesis of protein in microbial systems

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2898%2980042-6

7 January 2021

Efficient translation of tobacco mosaic virus RNA and rabbit globin 9S RNA in a cell-free system from commercial wheat germ

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2898%2980042-6

7 January 2021

Continuously coupled transcription-translation system for the production of rice cytoplasmic aldolase.

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2898%2980042-6

7 January 2021

An efficient mRNA-dependent translation system from reticulocyte lysates

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2898%2980042-6

7 January 2021

Effect of the ATP level on the overall protein biosynthesis rate in a wheat germ cell-free system

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2898%2980042-6

7 January 2021

Restoration of protein synthesis in lysed rabbit reticulocytes by the enzymatic removal of adenosine 5'-monophosphate with either AMP deaminase or AMP nucleosidase

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2898%2980042-6

7 January 2021

5'-adenosine monophosphate inhibits ternary complex formation by rat liver eIF-2

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2898%2980042-6

7 January 2021

Cooperativity of stabilized mRNA and enhanced translation activity in the cell-free system

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2898%2980042-6

7 January 2021

Activation of the heme-stabilized translational inhibitor of reticulocyte lysates by calcium ions and phospholipid

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2898%2980042-6

7 January 2021

Acetyl phosphate as an energy source for bacterial cell-free translation systems.

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2898%2980042-6

7 January 2021

Prolonged cell-free protein synthesis in a batch system using wheat germ extract

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2898%2980042-6

7 January 2021

A long-lived batch reaction system of cell-free protein synthesis

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2898%2980042-6

7 January 2021

An increased rate of cell-free protein synthesis by condensing wheat-germ extract with ultrafiltration membranes

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2898%2980042-6

7 January 2021

A highly efficient cell-free protein synthesis system from Escherichia coli

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2898%2980042-6

7 January 2021

Use of bacteriophage RNA polymerase in RNA synthesis

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2898%2980042-6

7 January 2021

In vitro selection and evolution of functional proteins by using ribosome display

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2898%2980042-6

7 January 2021

High-affinity peptide ligands to prostate-specific antigen identified by polysome selection

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2898%2980042-6

7 January 2021

44 Prokaryotic coupled transcription — translation

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2898%2980042-6

7 January 2021

A coupled in vitro transcription-translation system for the exclusive synthesis of polypeptides expressed from the T7 promoter.

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2898%2980042-6

7 January 2021

Use of T7 RNA polymerase in an optimized Escherichia coli coupled in vitro transcription-translation system. Application in regulatory studies and expression of long transcription units.

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2898%2980042-6

7 January 2021

Plasmid cDNA-directed protein synthesis in a coupled eukaryotic in vitro transcription-translation system.

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2898%2980042-6

7 January 2021

Gene expression in cell-free system on preparative scale

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2898%2980042-6

7 January 2021

Translation initiation in Escherichia coli: old and new questions

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2898%2980042-6

7 January 2021

In vitro scanning saturation mutagenesis of an antibody binding pocket

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2898%2980042-6

7 January 2021

Direct expression of PCR products in a cell-free transcription/translation system: synthesis of antibacterial peptide cecropin

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2898%2980042-6

7 January 2021

Selection of initiation sites by eucaryotic ribosomes: effect of inserting AUG triplets upstream from the coding sequence for preproinsulin

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2898%2980042-6

7 January 2021

Recognition of AUG and alternative initiator codons is augmented by G in position +4 but is not generally affected by the nucleotides in positions +5 and +6.

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2898%2980042-6

7 January 2021

Functional importance of RNA interactions in selection of translation initiation codons

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2898%2980042-6

7 January 2021

mRNA acetylated at 2'-OH-groups of ribose residues is functionally active in the cell-free translation system from wheat embryos

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2898%2980042-6

7 January 2021

Efficient expression of E. coli dihydrofolate reductase gene by an in vitro translation system using phosphorothioate mRNA.

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2898%2980042-6

7 January 2021

Phosphorothioate-containing RNAs show mRNA activity in the prokaryotic translation systems in vitro

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2898%2980042-6

7 January 2021

Structural determinants in AUF1 required for high affinity binding to A + U-rich elements

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2898%2980042-6

7 January 2021

Life and death in the cytoplasm: messages from the 3' end.

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2898%2980042-6

7 January 2021

Translation initiation factor eIF4G mediates in vitro poly(A) tail-dependent translation

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2898%2980042-6

7 January 2021

Translation initiation factors eIF-iso4G and eIF-4B interact with the poly(A)-binding protein and increase its RNA binding activity

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2898%2980042-6

7 January 2021

Interaction of polyadenylate-binding protein with the eIF4G homologue PAIP enhances translation

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2898%2980042-6

7 January 2021

Enhanced translation of chimaeric messenger RNAs containing a plant viral untranslated leader sequence

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2898%2980042-6

7 January 2021

The 5'-leader sequence of tobacco mosaic virus RNA enhances the expression of foreign gene transcripts in vitro and in vivo

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2898%2980042-6

7 January 2021

The 3' untranslated region of satellite tobacco necrosis virus RNA stimulates translation in vitro

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2898%2980042-6

7 January 2021

Enhancing effect of the 3'-untranslated region of tobacco mosaic virus RNA on protein synthesis in vitro

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2898%2980042-6

7 January 2021

mRNA translation: influence of the 5' and 3' untranslated regions

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2898%2980042-6

7 January 2021

Cis-acting elements and trans-acting factors for accurate translation of chloroplast psbA mRNAs: development of an in vitro translation system from tobacco chloroplasts.

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2898%2980042-6

7 January 2021

Translation-competent extracts from Saccharomyces cerevisiae: effects of L-A RNA, 5' cap, and 3' poly(A) tail on translational efficiency of mRNAs

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2898%2980042-6

7 January 2021

A common function for mRNA 5' and 3' ends in translation initiation in yeast

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2898%2980042-6

7 January 2021

A continuous cell-free translation system capable of producing polypeptides in high yield

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2898%2980042-6

7 January 2021

Synthesis of preparative amounts of biologically active interleukin-6 using a continuous-flow cell-free translation system

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2898%2980042-6

7 January 2021

A continuous cell-free protein synthesis system for coupled transcription-translation.

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2898%2980042-6

7 January 2021

A semicontinuous prokaryotic coupled transcription/translation system using a dialysis membrane

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2898%2980042-6

7 January 2021

Gene expression in a cell-free system on the preparative scale

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2898%2980042-6

7 January 2021

Energy charge of the adenylate pool as a regulatory parameter. Interaction with feedback modifiers

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2898%2980042-6

7 January 2021

Dihydrofolate reductase synthesis in the presence of immobilized methotrexate. An approach to a continuous cell-free protein synthesis system.

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2898%2980042-6

7 January 2021

Cotranslational folding of proteins

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2898%2980042-6

7 January 2021

Cotranslational protein folding

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2898%2980042-6

7 January 2021

Recombination of protein domains facilitated by co-translational folding in eukaryotes.

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2898%2980042-6

7 January 2021

The importance of the N-terminal segment for DnaJ-mediated folding of rhodanese while bound to ribosomes as peptidyl-tRNA.

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2898%2980042-6

7 January 2021

Folding of firefly luciferase during translation in a cell-free system.

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2898%2980042-6

7 January 2021

Enzymatic activity of the ribosome-bound nascent polypeptide

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2898%2980042-6

7 January 2021

Cotranslational folding of globin

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2898%2980042-6

7 January 2021

Antibody-ribosome-mRNA (ARM) complexes as efficient selection particles for in vitro display and evolution of antibody combining sites

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2898%2980042-6

7 January 2021

Free luciferase may acquire a more favorable conformation than ribosome-associated luciferase for its activity expression

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2898%2980042-6

7 January 2021

Protein biogenesis: chaperones for nascent polypeptides

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2898%2980042-6

7 January 2021

Building bridges: disulphide bond formation in the cell

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2898%2980042-6

7 January 2021

Functional antibody production using cell-free translation: effects of protein disulfide isomerase and chaperones.

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2898%2980042-6

7 January 2021

In vitro protein folding by ribosomes from Escherichia coli, wheat germ and rat liver: the role of the 50S particle and its 23S rRNA.

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2898%2980042-6

7 January 2021

Ribosomes and ribosomal RNA as chaperones for folding of proteins.

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2898%2980042-6

7 January 2021

Reactivation of denatured proteins by 23S ribosomal RNA: role of domain V.

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2898%2980042-6

7 January 2021

Renaturation of rhodanese by translational elongation factor (EF) Tu. Protein refolding by EF-Tu flexing

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2898%2980042-6

7 January 2021

Preparation and application of chaperone-deficient Escherichia coli cell-free translation systems

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2898%2980042-6

7 January 2021

Site-directed mutagenesis with an expanded genetic code.

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2898%2980042-6

7 January 2021

Noncoded amino acid replacement probes of the aspartate aminotransferase mechanism

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2898%2980042-6

7 January 2021

Synthesis of region-labelled proteins for NMR studies by in vitro translation of column-coupled mRNAs

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2898%2980042-6

7 January 2021

Protein synthesis by chemical ligation of unprotected peptides in aqueous solution

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2898%2980042-6

7 January 2021

Protein splicing: self-splicing of genetically mobile elements at the protein level.

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2898%2980042-6

7 January 2021

Translation arrest by oligodeoxynucleotides complementary to mRNA coding sequences yields polypeptides of predetermined length

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2898%2980042-6

7 January 2021

The clinical and molecular genetic approach to Duchenne and Becker muscular dystrophy: an updated protocol

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2898%2980042-6

7 January 2021

Preparation of microsomal membranes for cotranslational protein translocation.

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2898%2980042-6

7 January 2021

In vitro reconstitution of assembly of apolipoprotein B48-containing lipoproteins

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2898%2980042-6

7 January 2021

In vitro translation and assembly of a complete T cell receptor-CD3 complex

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2898%2980042-6

7 January 2021

Membrane insertion, glycosylation, and oligomerization of inositol trisphosphate receptors in a cell-free translation system

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2898%2980042-6

7 January 2021

Cell-free synthesis and assembly of connexins into functional gap junction membrane channels

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2898%2980042-6

7 January 2021

Cell-free, de novo synthesis of poliovirus

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2898%2980042-6

7 January 2021

A multistep, ATP-dependent pathway for assembly of human immunodeficiency virus capsids in a cell-free system

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2898%2980042-6

7 January 2021

Formation of bacteriophage MS2 infectious units in a cell-free translation system.

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2898%2980042-6

7 January 2021

Genetic recombination of poliovirus in a cell-free system

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2898%2980042-6

7 January 2021

Duck Hepatitis B Virus polymerase produced by in vitro transcription and translation possesses DNA polymerase and reverse transcriptase activities

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2898%2980042-6

7 January 2021

The reverse transcriptase of hepatitis B virus acts as a protein primer for viral DNA synthesis

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2898%2980042-6

7 January 2021

An in vitro polysome display system for identifying ligands from very large peptide libraries

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2898%2980042-6

7 January 2021

Cell-free synthesis of peptide libraries displayed on polysomes

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2898%2980042-6

7 January 2021

Biologically and chemically pure mRNA coding for a mouse immunoglobulin L-chain prepared with the aid of antibodies and immobilized oligothymidine

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2898%2980042-6

7 January 2021

Improvements in Immunoprecipitation of Specific Messenger RNA. Isolation of Highly Purified Conalbumin mRNA in High Yield

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2898%2980042-6

7 January 2021

cDNA clones for the heavy chain of HLA-DR antigens obtained after immunopurification of polysomes by monoclonal antibody

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2898%2980042-6

7 January 2021

Purification of low-abundance messenger RNAs from rat liver by polysome immunoadsorption

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2898%2980042-6

7 January 2021

Ribosome display efficiently selects and evolves high-affinity antibodies in vitro from immune libraries.

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2898%2980042-6

7 January 2021

In vitro virus: bonding of mRNA bearing puromycin at the 3'-terminal end to the C-terminal end of its encoded protein on the ribosome in vitro

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2898%2980042-6

7 January 2021

RNA-peptide fusions for the in vitro selection of peptides and proteins

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2898%2980042-6

7 January 2021

10.1016/S0958-1669(98)80042-6

Identifiers

DOI

1 reference

5 July 2017

1 reference

PubMed ID

9821285