Strategies for the purification and on-column cleavage of glutathione-S-transferase fusion target proteins. (Q32076882)

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9 December 2019

title

Strategies for the purification and on-column cleavage of glutathione-S-transferase fusion target proteins (English)

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9 December 2019

1

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9 December 2019

author name string

Said Eshaghi

2

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9 December 2019

Thomas Urbig

3

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9 December 2019

Sean McSweeney

4

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9 December 2019

Anna Heijbel

5

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9 December 2019

Gilles Salbert

6

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9 December 2019

Darcy Birse

7

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9 December 2019

publication date

1 March 2002

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Journal of Chromatography B Analytical Technologies in the Biomedical and Life Sciences

9 December 2019

published in

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9 December 2019

volume

769

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9 December 2019

issue

1

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9 December 2019

page(s)

133-144

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Affinity fusion strategies for detection, purification, and immobilization of recombinant proteins

9 December 2019

cites work

1 reference

https://api.crossref.org/works/10.1016%2FS1570-0232%2801%2900637-7

7 January 2021

Recombinant protein expression in Escherichia coli

1 reference

https://api.crossref.org/works/10.1016%2FS1570-0232%2801%2900637-7

7 January 2021

Escherichia coli maltose-binding protein is uncommonly effective at promoting the solubility of polypeptides to which it is fused

1 reference

https://api.crossref.org/works/10.1016%2FS1570-0232%2801%2900637-7

7 January 2021

Advances in refolding of proteins produced in E. coli

1 reference

https://api.crossref.org/works/10.1016%2FS1570-0232%2801%2900637-7

7 January 2021

Eukaryotic proteins expressed in Escherichia coli: An improved thrombin cleavage and purification procedure of fusion proteins with glutathione S-transferase

1 reference

https://api.crossref.org/works/10.1016%2FS1570-0232%2801%2900637-7

7 January 2021

Expression and purification of recombinant 3C proteinase of Coxsackievirus B3

1 reference

https://api.crossref.org/works/10.1016%2FS1570-0232%2801%2900637-7

7 January 2021

Gene fusion expression systems in Escherichia coli

1 reference

https://api.crossref.org/works/10.1016%2FS1570-0232%2801%2900637-7

7 January 2021

Protein splicing involving the Saccharomyces cerevisiae VMA intein. The steps in the splicing pathway, side reactions leading to protein cleavage, and establishment of an in vitro splicing system

1 reference

https://api.crossref.org/works/10.1016%2FS1570-0232%2801%2900637-7

7 January 2021

Single-step trypsin cleavage of a fusion protein to obtain human insulin and its C peptide

1 reference

https://api.crossref.org/works/10.1016%2FS1570-0232%2801%2900637-7

7 January 2021

Integrated production of human insulin and its C-peptide.

1 reference

https://api.crossref.org/works/10.1016%2FS1570-0232%2801%2900637-7

7 January 2021

Production of a thermostable DNA polymerase by site-specific cleavage of a heat-eluted affinity fusion protein

1 reference

https://api.crossref.org/works/10.1016%2FS1570-0232%2801%2900637-7

7 January 2021

Efficient and rapid affinity purification of proteins using recombinant fusion proteases

1 reference

https://api.crossref.org/works/10.1016%2FS1570-0232%2801%2900637-7

7 January 2021

Genetic Approach to Facilitate Purification of Recombinant Proteins with a Novel Metal Chelate Adsorbent

1 reference

https://api.crossref.org/works/10.1016%2FS1570-0232%2801%2900637-7

7 January 2021

New metal chelate adsorbent selective for proteins and peptides containing neighbouring histidine residues

1 reference

https://api.crossref.org/works/10.1016%2FS1570-0232%2801%2900637-7

7 January 2021

A Short Polypeptide Marker Sequence Useful for Recombinant Protein Identification and Purification

1 reference

https://api.crossref.org/works/10.1016%2FS1570-0232%2801%2900637-7

7 January 2021

A thioredoxin gene fusion expression system that circumvents inclusion body formation in the E. coli cytoplasm.

1 reference

https://api.crossref.org/works/10.1016%2FS1570-0232%2801%2900637-7

7 January 2021

Purification by immobilized metal affinity chromatography of human atrial natriuretic peptide expressed in a novel thioredoxin fusion protein.

1 reference

https://api.crossref.org/works/10.1016%2FS1570-0232%2801%2900637-7

7 January 2021

Competitive elution of protein A fusion proteins allows specific recovery under mild conditions.

1 reference

https://api.crossref.org/works/10.1016%2FS1570-0232%2801%2900637-7

7 January 2021

Enhanced in vitro refolding of insulin-like growth factor I using a solubilizing fusion partner

1 reference

https://api.crossref.org/works/10.1016%2FS1570-0232%2801%2900637-7

7 January 2021

Gene fusion vectors based on the gene for staphylococcal protein A.

1 reference

https://api.crossref.org/works/10.1016%2FS1570-0232%2801%2900637-7

7 January 2021

Multiple affinity domains for the detection, purification and immobilization of recombinant proteins.

1 reference

https://api.crossref.org/works/10.1016%2FS1570-0232%2801%2900637-7

7 January 2021

The random peptide library-assisted engineering of a C-terminal affinity peptide, useful for the detection and purification of a functional Ig Fv fragment.

1 reference

https://api.crossref.org/works/10.1016%2FS1570-0232%2801%2900637-7

7 January 2021

Production in Escherichia coli and one-step purification of bifunctional hybrid proteins which bind maltose. Export of the Klenow polymerase into the periplasmic space

1 reference

https://api.crossref.org/works/10.1016%2FS1570-0232%2801%2900637-7

7 January 2021

Vectors that facilitate the expression and purification of foreign peptides in Escherichia coli by fusion to maltose-binding protein

1 reference

https://api.crossref.org/works/10.1016%2FS1570-0232%2801%2900637-7

7 January 2021

An Escherichia coli vector to express and purify foreign proteins by fusion to and separation from maltose-binding protein

1 reference

https://api.crossref.org/works/10.1016%2FS1570-0232%2801%2900637-7

7 January 2021

Peptide and protein molecular weight determination by electrophoresis using a high-molarity tris buffer system without urea

1 reference

https://api.crossref.org/works/10.1016%2FS1570-0232%2801%2900637-7

7 January 2021

A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding

1 reference

https://api.crossref.org/works/10.1016%2FS1570-0232%2801%2900637-7

7 January 2021

The HeLa Pur factor binds single-stranded DNA at a specific element conserved in gene flanking regions and origins of DNA replication

1 reference

https://api.crossref.org/works/10.1016%2FS1570-0232%2801%2900637-7

7 January 2021

Activation of the JC virus Tat-responsive transcriptional control element by association of the Tat protein of human immunodeficiency virus 1 with cellular protein Pur alpha

1 reference

https://api.crossref.org/works/10.1016%2FS1570-0232%2801%2900637-7

7 January 2021

Transcriptional regulation of neuronal nicotinic acetylcholine receptor genes. A possible role for the DNA-binding protein Puralpha.

1 reference

https://api.crossref.org/works/10.1016%2FS1570-0232%2801%2900637-7

7 January 2021

Calmodulin functions as an activator of Pur alpha binding to single-stranded purine-rich DNA elements (PUR elements)

1 reference

https://api.crossref.org/works/10.1016%2FS1570-0232%2801%2900637-7

7 January 2021

The single-stranded DNA binding protein, Pur-alpha, binds HIV-1 TAR RNA and activates HIV-1 transcription

1 reference

https://api.crossref.org/works/10.1016%2FS1570-0232%2801%2900637-7

7 January 2021

Induced folding in RNA-protein recognition: more than a simple molecular handshake

1 reference

https://api.crossref.org/works/10.1016%2FS1570-0232%2801%2900637-7

7 January 2021

Testis/brain RNA-binding protein attaches translationally repressed and transported mRNAs to microtubules

1 reference

https://api.crossref.org/works/10.1016%2FS1570-0232%2801%2900637-7

7 January 2021

A novel gene, Translin, encodes a recombination hotspot binding protein associated with chromosomal translocations

1 reference

https://api.crossref.org/works/10.1016%2FS1570-0232%2801%2900637-7

7 January 2021

A novel multi-functional chloroplast protein: identification of a 40 kDa immunophilin-like protein located in the thylakoid lumen

1 reference

https://api.crossref.org/works/10.1016%2FS1570-0232%2801%2900637-7

7 January 2021

A cyclophilin-regulated PP2A-like protein phosphatase in thylakoid membranes of plant chloroplasts

1 reference

https://api.crossref.org/works/10.1016%2FS1570-0232%2801%2900637-7

7 January 2021

Dephosphorylation of photosystem II reaction center proteins in plant photosynthetic membranes as an immediate response to abrupt elevation of temperature.

1 reference

https://api.crossref.org/works/10.1016%2FS1570-0232%2801%2900637-7

7 January 2021

Chick ovalbumin upstream promoter-transcription factors (COUP-TFs): coming of age

1 reference

https://api.crossref.org/works/10.1016%2FS1570-0232%2801%2900637-7

7 January 2021

Expression screening reveals an orphan receptor chick ovalbumin upstream promoter transcription factor I as a regulator of neurite/substrate-cell contacts and cell aggregation

1 reference

https://api.crossref.org/works/10.1016%2FS1570-0232%2801%2900637-7

7 January 2021

Null mutation of mCOUP-TFI results in defects in morphogenesis of the glossopharyngeal ganglion, axonal projection, and arborization.

1 reference

https://api.crossref.org/works/10.1016%2FS1570-0232%2801%2900637-7

7 January 2021

COUP-TFI (chicken ovalbumin upstream promoter-transcription factor I) regulates cell migration and axogenesis in differentiating P19 embryonal carcinoma cells.

1 reference

https://api.crossref.org/works/10.1016%2FS1570-0232%2801%2900637-7

7 January 2021

Differential expression of an orphan receptor COUP-TFI and corepressors in adrenal tumors

1 reference

https://api.crossref.org/works/10.1016%2FS1570-0232%2801%2900637-7

7 January 2021

Erratum: Crystal structure of a bacterialsignal peptidase in complex with a β-lactam inhibitor

1 reference

https://api.crossref.org/works/10.1016%2FS1570-0232%2801%2900637-7

7 January 2021

Distant downstream sequence determinants can control N-tail translocation during protein insertion into the endoplasmic reticulum membrane

1 reference

https://api.crossref.org/works/10.1016%2FS1570-0232%2801%2900637-7

7 January 2021

An Escherichia coli homologue of eukaryotic potassium channel proteins

1 reference

https://api.crossref.org/works/10.1016%2FS1570-0232%2801%2900637-7

7 January 2021

Membrane topology of Kch, a putative K+ channel from Escherichia coli.

1 reference

https://api.crossref.org/works/10.1016%2FS1570-0232%2801%2900637-7

7 January 2021

Structures by numbers

1 reference

https://api.crossref.org/works/10.1016%2FS1570-0232%2801%2900637-7

7 January 2021

Protein production: feeding the crystallographers and NMR spectroscopists

1 reference

https://api.crossref.org/works/10.1016%2FS1570-0232%2801%2900637-7

7 January 2021

High-throughput protein crystallization

1 reference

https://api.crossref.org/works/10.1016%2FS1570-0232%2801%2900637-7

7 January 2021

Automation of X-ray crystallography

1 reference

https://api.crossref.org/works/10.1016%2FS1570-0232%2801%2900637-7

7 January 2021

Automated crystal mounting and data collection for protein crystallography

1 reference

https://api.crossref.org/works/10.1016%2FS1570-0232%2801%2900637-7

7 January 2021

10.1016/S1570-0232(01)00637-7

Identifiers

DOI

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9 December 2019

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