Site-directed, Ligase-Independent Mutagenesis (SLIM): a single-tube methodology approaching 100% efficiency in 4 h. (Q33209678)

23 July 2017

Site-directed, Ligase-Independent Mutagenesis (SLIM): a single-tube methodology approaching 100% efficiency in 4 h. (English)

1 reference

23 July 2017

Joyce Chiu

1

1 reference

23 July 2017

Paul E March

series ordinal

2

1 reference

23 July 2017

Ryan Lee

series ordinal

3

1 reference

23 July 2017

Daniel Tillett

series ordinal

4

1 reference

23 July 2017

language of work or name

English

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publication date

7 December 2004

1 reference

23 July 2017

Nucleic Acids Research

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23 July 2017

32

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23 July 2017

e174

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23 July 2017

21

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Enzyme-free cloning: a rapid method to clone PCR products independent of vector restriction enzyme sites

23 July 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=535700

An efficient 1-tube PCR method for internal site-directed mutagenesis of large amplified molecules

21 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=535700

Rapid and efficient site-specific mutagenesis without phenotypic selection

21 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=535700

Construction of mutant and chimeric genes using the polymerase chain reaction

21 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=535700

Engineering hybrid genes without the use of restriction enzymes: gene splicing by overlap extension

21 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=535700

A simple method for site-directed mutagenesis using the polymerase chain reaction

21 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=535700

Rapid insertional mutagenesis of DNA by polymerase chain reaction (PCR).

21 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=535700

High-resolution epitope mapping of hGH-receptor interactions by alanine-scanning mutagenesis

21 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=535700

Receptor and antibody epitopes in human growth hormone identified by homolog-scanning mutagenesis.

21 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=535700

The "megaprimer" method of site-directed mutagenesis

21 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=535700

A general method for rapid site-directed mutagenesis using the polymerase chain reaction.

21 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=535700

Site-directed mutagenesis using a double-stranded DNA fragment as a PCR primer

21 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=535700

Site-directed mutagenesis of virtually any plasmid by eliminating a unique site

21 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=535700

Enzymatic inverse PCR: a restriction site independent, single-fragment method for high-efficiency, site-directed mutagenesis

21 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=535700

PCR-based random mutagenesis method using spiked oligonucleotides to randomize selected parts of a gene without any wild-type background

21 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=535700

Site-directed mutagenesis: a two-step method using PCR and DpnI

31 October 2018

1 reference

12 December 2020

Generation of multiple site-specific mutations in a single polymerase chain reaction product

1 reference

12 December 2020

Inverse PCR-generated internally deleted constructs for direct characterization of promoter regulatory regions

1 reference

12 December 2020

Site-directed mutagenesis

1 reference

12 December 2020

Site-directed mutagenesis of double-stranded DNA by the polymerase chain reaction

1 reference

12 December 2020

Identifiers

DOI

10.1093/NAR/GNH172

1 reference

23 July 2017

1 reference

23 July 2017

1 reference