Selecting and screening recombinant antibody libraries (Q33223036)

24 July 2017

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title

Selecting and screening recombinant antibody libraries (English)

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24 July 2017

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Hennie R Hoogenboom

1

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24 July 2017

1 September 2005

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24 July 2017

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24 July 2017

23

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24 July 2017

1105-1116

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24 July 2017

9

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https://scigraph.springernature.com/pub.10.1038/nbt1126

24 July 2017

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7 January 2021

Cloning immunoglobulin variable domains for expression by the polymerase chain reaction

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7 January 2021

Binding activities of a repertoire of single immunoglobulin variable domains secreted from Escherichia coli

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7 January 2021

Generation of a large combinatorial library of the immunoglobulin repertoire in phage lambda

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7 January 2021

Phage antibodies: filamentous phage displaying antibody variable domains

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7 January 2021

Making antibody fragments using phage display libraries

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7 January 2021

By-passing immunization. Human antibodies from V-gene libraries displayed on phage

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7 January 2021

A large array of human monoclonal antibodies to type 1 human immunodeficiency virus from combinatorial libraries of asymptomatic seropositive individuals

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7 January 2021

Making antibodies by phage display technology

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7 January 2021

Isolation of high affinity human antibodies directly from large synthetic repertoires.

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7 January 2021

Human antibodies with sub-nanomolar affinities isolated from a large non-immunized phage display library

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7 January 2021

A large non-immunized human Fab fragment phage library that permits rapid isolation and kinetic analysis of high affinity antibodies

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7 January 2021

Fully synthetic human combinatorial antibody libraries (HuCAL) based on modular consensus frameworks and CDRs randomized with trinucleotides

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7 January 2021

Generation of high-affinity human antibodies by combining donor-derived and synthetic complementarity-determining-region diversity.

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7 January 2021

CDR walking mutagenesis for the affinity maturation of a potent human anti-HIV-1 antibody into the picomolar range

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7 January 2021

Isolation of picomolar affinity anti-c-erbB-2 single-chain Fv by molecular evolution of the complementarity determining regions in the center of the antibody binding site

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7 January 2021

Tailoring in vitro selection for a picomolar affinity human antibody directed against vascular endothelial growth factor receptor 2 for enhanced neutralizing activity

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7 January 2021

Direct selection of a human antibody fragment directed against the tumor T-cell epitope HLA-A1-MAGE-A1 from a nonimmunized phage-Fab library.

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7 January 2021

Antibodies to human fetal erythroid cells from a nonimmune phage antibody library

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7 January 2021

Broadly cross-reactive HIV-1-neutralizing human monoclonal Fab selected for binding to gp120-CD4-CCR5 complexes

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7 January 2021

The human antibody repertoire specific for rabies virus glycoprotein as selected from immune libraries

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7 January 2021

In-vitro protein evolution by ribosome display and mRNA display

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7 January 2021

Picomolar affinity antibodies from a fully synthetic naive library selected and evolved by ribosome display

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7 January 2021

In vitro generated antibodies specific for telomeric guanine-quadruplex DNA react with Stylonychia lemnae macronuclei

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7 January 2021

Ribosome display efficiently selects and evolves high-affinity antibodies in vitro from immune libraries.

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7 January 2021

Directed in vitro evolution and crystallographic analysis of a peptide-binding single chain antibody fragment (scFv) with low picomolar affinity

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7 January 2021

Yeast surface display for screening combinatorial polypeptide libraries

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7 January 2021

Directed evolution of antibody fragments with monovalent femtomolar antigen-binding affinity

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7 January 2021

Development of an optimized expression system for the screening of antibody libraries displayed on the Escherichia coli surface.

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7 January 2021

Isolation of high-affinity ligand-binding proteins by periplasmic expression with cytometric screening (PECS).

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7 January 2021

Anchored periplasmic expression, a versatile technology for the isolation of high-affinity antibodies from Escherichia coli-expressed libraries

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7 January 2021

Selection of functional human antibodies from retroviral display libraries

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7 January 2021

CIS display: In vitro selection of peptides from libraries of protein-DNA complexes

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7 January 2021

Covalent antibody display--an in vitro antibody-DNA library selection system

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7 January 2021

Microbead display by in vitro compartmentalisation: selection for binding using flow cytometry

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7 January 2021

Fast selection of antibodies without antigen purification: adaptation of the protein fragment complementation assay to select antigen-antibody pairs.

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7 January 2021

Cell growth selection system to detect extracellular and transmembrane protein interactions

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7 January 2021

Ligand binding of a ribosome-displayed protein detected in solution at the single molecule level by fluorescence correlation spectroscopy

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7 January 2021

Engineering novel binding proteins from nonimmunoglobulin domains

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7 January 2021

Generation and iterative affinity maturation of antibodies in vitro using hypermutating B-cell lines

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7 January 2021

Hypermutation in human B cells in vivo and in vitro.

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7 January 2021

Antibodies from phage antibody libraries

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7 January 2021

Model systems to study the parameters determining the success of phage antibody selections on complex antigens

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7 January 2021

Fine affinity discrimination by yeast surface display and flow cytometry

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7 January 2021

Dimerization of Fab fragments enables ready screening of phage antibodies that affect hepatocyte growth factor/scatter factor activity on target cells

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7 January 2021

Evolving phage vectors for cell targeted gene delivery

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7 January 2021

Direct selection for a catalytic mechanism from combinatorial antibody libraries

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7 January 2021

Toward selection of internalizing antibodies from phage libraries

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7 January 2021

Yeast display of antibody fragments: a discovery and characterization platform.

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7 January 2021

Antibodies in proteomics II: screening, high-throughput characterization and downstream applications

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7 January 2021

High-throughput antibody production

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7 January 2021

Antibodies in haystacks: how selection strategy influences the outcome of selection from molecular diversity libraries

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7 January 2021

Towards proteome-wide production of monoclonal antibody by phage display

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7 January 2021

High-throughput screening of surface displayed gene products

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7 January 2021

Target validation for genomics using peptide-specific phage antibodies: a study of five gene products overexpressed in colorectal cancer

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7 January 2021

From EST to IHC: human antibody pipeline for target research

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7 January 2021

The Remarkable Flexibility of the Human Antibody Repertoire; Isolation of Over One Thousand Different Antibodies to a Single Protein, BLyS

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7 January 2021

Seeing better through a MIST: evaluation of monoclonal recombinant antibody fragments on microarrays.

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7 January 2021

Rescue and in situ selection and evaluation (RISE): a method for high-throughput panning of phage display libraries

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7 January 2021

High-throughput, cloning-independent protein library construction by combining single-molecule DNA amplification with in vitro expression.

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7 January 2021

By-passing selection: direct screening for antibody-antigen interactions using protein arrays

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7 January 2021

Discovery of human antibodies to cell surface antigens by capture lift screening of phage-expressed antibody libraries

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7 January 2021

Phage display and colony filter screening for high-throughput selection of antibody libraries.

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7 January 2021

Analyzing antibody specificity with whole proteome microarrays

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7 January 2021

Protein microarrays for antibody profiling: specificity and affinity determination on a chip

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7 January 2021

Rapid generation of functional human IgG antibodies derived from Fab-on-phage display libraries

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7 January 2021

An integrated vector system for the eukaryotic expression of antibodies or their fragments after selection from phage display libraries

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7 January 2021

Oligonucleotide-assisted cleavage and ligation: a novel directional DNA cloning technology to capture cDNAs. Application in the construction of a human immune antibody phage-display library

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7 January 2021

Molecular dissection of the tissue transglutaminase autoantibody response in celiac disease.

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7 January 2021

Evidence for a bias toward intracellular antigens in the local humoral anti-tumor immune response of a colorectal cancer patient revealed by phage display

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7 January 2021

By-passing immunisation. Human antibodies from synthetic repertoires of germline VH gene segments rearranged in vitro

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7 January 2021

Semisynthetic combinatorial antibody libraries: a chemical solution to the diversity problem

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7 January 2021

Phage-displayed antibody libraries of synthetic heavy chain complementarity determining regions

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7 January 2021

Design, construction, and characterization of a large synthetic human antibody phage display library

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7 January 2021

Antibody arrays for high-throughput screening of antibody-antigen interactions

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7 January 2021

Construction, evaluation and refinement of a large human antibody phage library based on the IgD and IgM variable gene repertoire.

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7 January 2021

Recombining germline-derived CDR sequences for creating diverse single-framework antibody libraries

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7 January 2021

Mating antibody phage display with proteomics

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7 January 2021

Natural and designer binding sites made by phage display technology

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7 January 2021

Diversity in the CDR3 region of V(H) is sufficient for most antibody specificities

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7 January 2021

Combinatorial immunoglobulin light chain variability creates sufficient B cell diversity to mount protective antibody responses against pathogen infections.

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7 January 2021

Selection of scFvs specific for HBV DNA polymerase using ribosome display.

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7 January 2021

Selection of a human anti-progesterone antibody fragment from a transgenic mouse library by ARM ribosome display

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7 January 2021

Novel human monoclonal antibody combination effectively neutralizing natural rabies virus variants and individual in vitro escape mutants

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7 January 2021

Ultra-potent antibodies against respiratory syncytial virus: effects of binding kinetics and binding valence on viral neutralization

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7 January 2021

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7 January 2021

Stepwise in vitro affinity maturation of Vitaxin, an alphav beta3-specific humanized mAb.

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7 January 2021

In vitro antibody evolution targeting germline hot spots to increase activity of an anti-CD22 immunotoxin

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7 January 2021

Kinetic and affinity limits on antibodies produced during immune responses

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7 January 2021

Design and use of a phage display library. Human antibodies with subnanomolar affinity against a marker of angiogenesis eluted from a two-dimensional gel.

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7 January 2021

Human combinatorial Fab library yielding specific and functional antibodies against the human fibroblast growth factor receptor 3.

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7 January 2021

Substantial energetic improvement with minimal structural perturbation in a high affinity mutant antibody

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7 January 2021

Antibody humanization using monovalent phage display

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7 January 2021

Humanization of a murine monoclonal antibody by simultaneous optimization of framework and CDR residues.

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7 January 2021

Antibody humanization by framework shuffling.

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7 January 2021

Guided selection of a pan carcinoma specific antibody reveals similar binding characteristics yet structural divergence between the original murine antibody and its human equivalent

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7 January 2021

In vitro and in vivo activity of MT201, a fully human monoclonal antibody for pancarcinoma treatment

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7 January 2021

A phage display approach for rapid antibody humanization: designed combinatorial V gene libraries.

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7 January 2021

Selection for improved protein stability by phage display.

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7 January 2021

Proteolytic selection for protein folding using filamentous bacteriophages

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7 January 2021

Selecting for antibody scFv fragments with improved stability using phage display with denaturation under reducing conditions

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7 January 2021

Aggregation-resistant domain antibodies selected on phage by heat denaturation

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7 January 2021

Increasing the secretory capacity of Saccharomyces cerevisiae for production of single-chain antibody fragments.

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7 January 2021

Directed evolution of a stable scaffold for T-cell receptor engineering.

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7 January 2021

Directed evolution of an anti-carcinoembryonic antigen scFv with a 4-day monovalent dissociation half-time at 37 degrees C.

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7 January 2021

Directed evolution of proteins for heterologous expression and stability.

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7 January 2021

Identification of colon tumour-associated antigens by phage antibody selections on primary colorectal carcinoma

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7 January 2021

Isolation of human antibodies to tumor-associated endothelial cell markers by in vitro human endothelial cell selection with phage display libraries.

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7 January 2021

Mapping tumor epitope space by direct selection of single-chain Fv antibody libraries on prostate cancer cells

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7 January 2021

Recombinant antibodies against subcellular fractions used to track endogenous Golgi protein dynamics in vivo

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7 January 2021

A proteomic approach to tumour target identification using phage display, affinity purification and mass spectrometry.

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7 January 2021

C-type lectin-like molecule-1: a novel myeloid cell surface marker associated with acute myeloid leukemia

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7 January 2021

Intracellular antibodies for proteomics

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7 January 2021

Expression of an antibody fragment at high levels in the bacterial cytoplasm.

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7 January 2021

Rescue of a neutralizing anti-viral antibody fragment from an intracellular polyclonal repertoire expressed in mammalian cells.

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7 January 2021

Direct phage to intrabody screening (DPIS): demonstration by isolation of cytosolic intrabodies against the TES1 site of Epstein Barr virus latent membrane protein 1 (LMP1) that block NF-kappaB transactivation

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7 January 2021

Selection of antibodies for intracellular function using a two-hybrid in vivo system

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7 January 2021

Direct in vivo screening of intrabody libraries constructed on a highly stable single-chain framework

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7 January 2021

Single domain intracellular antibodies: a minimal fragment for direct in vivo selection of antigen-specific intrabodies

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7 January 2021

Intracellular kinase inhibitors selected from combinatorial libraries of designed ankyrin repeat proteins

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7 January 2021

Expressed murine and human CDR-H3 intervals of equal length exhibit distinct repertoires that differ in their amino acid composition and predicted range of structures

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7 January 2021

Synthetic antibodies from a four-amino-acid code: a dominant role for tyrosine in antigen recognition

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7 January 2021

Molecular recognition by a binary code

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7 January 2021

Integrating cell-level kinetic modeling into the design of engineered protein therapeutics.

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7 January 2021

Kinetic and affinity predictions of a protein-protein interaction using multivariate experimental design

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7 January 2021

A structure-based database of antibody variable domain diversity.

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7 January 2021

Identification of differences in the specificity-determining residues of antibodies that recognize antigens of different size: implications for the rational design of antibody repertoires.

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7 January 2021

Towards the design of an antibody that recognises a given protein epitope

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7 January 2021

Antibody domain exchange is an immunological solution to carbohydrate cluster recognition

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7 January 2021

Crystal structure of the broadly cross-reactive HIV-1-neutralizing Fab X5 and fine mapping of its epitope

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7 January 2021

Selection of optical biosensors from chemisynthetic antibody libraries

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7 January 2021

TCR-like human antibodies expressed on human CTLs mediate antibody affinity-dependent cytolytic activity.

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7 January 2021

Selection and analysis of an optimized anti-VEGF antibody: crystal structure of an affinity-matured Fab in complex with antigen

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7 January 2021

Potent inhibition of huntingtin aggregation and cytotoxicity by a disulfide bond-free single-domain intracellular antibody

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7 January 2021

A semi-synthetic repertoire of intrinsically stable antibody fragments derived from a single-framework scaffold

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7 January 2021

A human synthetic combinatorial library of arrayable single-chain antibodies based on shuffling in vivo formed CDRs into general framework regions.

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7 January 2021

Affinity maturation of a V(H)H by mutational hotspot randomization

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7 January 2021

Protection against anthrax toxin by recombinant antibody fragments correlates with antigen affinity

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7 January 2021

Exploiting recombination in single bacteria to make large phage antibody libraries

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7 January 2021

Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements

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7 January 2021

Phage diabody repertoires for selection of large numbers of bispecific antibody fragments

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7 January 2021

Bivalent antibody phage display mimics natural immunoglobulin

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7 January 2021

Targeted gene delivery to mammalian cells by filamentous bacteriophage

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7 January 2021

Phage versus phagemid libraries for generation of human monoclonal antibodies.

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7 January 2021

Isolation of receptor-ligand pairs by capture of long-lived multivalent interaction complexes

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7 January 2021

An in vitro polysome display system for identifying ligands from very large peptide libraries

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7 January 2021

Antibody-ribosome-mRNA (ARM) complexes as efficient selection particles for in vitro display and evolution of antibody combining sites

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7 January 2021

In vitro selection and evolution of functional proteins by using ribosome display

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7 January 2021

The use of mRNA display to select high-affinity protein-binding peptides

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7 January 2021

Directed evolution of high-affinity antibody mimics using mRNA display

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7 January 2021

Flow-cytometric isolation of human antibodies from a nonimmune Saccharomyces cerevisiae surface display library

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7 January 2021

Quantitative screening of yeast surface-displayed polypeptide libraries by magnetic bead capture

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7 January 2021

Affinity maturation of Fab antibody fragments by fluorescent-activated cell sorting of yeast-displayed libraries

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7 January 2021

Construction and diversification of yeast cell surface displayed libraries by yeast mating: application to the affinity maturation of Fab antibody fragments

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7 January 2021

Shuffled antibody libraries created by in vivo homologous recombination and yeast surface display

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7 January 2021

Identifiers

DOI

10.1038/NBT1126

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24 July 2017

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PubMed ID

16151404