Optimization of in vitro transcription and full-length cDNA synthesis using the T4 bacteriophage gene 32 protein (Q33233252)

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16 January 2020

title

Optimization of in vitro transcription and full-length cDNA synthesis using the T4 bacteriophage gene 32 protein (English)

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16 January 2020

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Caroline Piché

1

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16 January 2020

Johann P Schernthaner

series ordinal

2

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16 January 2020

publication date

1 September 2005

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Journal of Biomolecular Techniques

16 January 2020

published in

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16 January 2020

volume

16

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16 January 2020

issue

3

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16 January 2020

page(s)

239-247

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describes a project that uses

16 January 2020

ImageQuant

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11 June 2022

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inferred from PubMed Central ID database lookup

cites work

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Improved method for the construction of full-length enriched cDNA libraries

21 June 2018

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Extra-long first-strand cDNA synthesis

21 June 2018

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Full-length cDNAs: more than just reaching the ends

21 June 2018

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Comparative evaluation of 5'-end-sequence quality of clones in CAP trapper and other full-length-cDNA libraries

21 June 2018

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Escherichia coli CspA-family RNA chaperones are transcription antiterminators

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High-efficiency full-length cDNA cloning

21 June 2018

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Evidence of interactions between the nucleocapsid protein NCp7 and the reverse transcriptase of HIV-1.

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High-efficiency cloning of Arabidopsis full-length cDNA by biotinylated CAP trapper

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Reverse transcriptase (RT) inhibition of PCR at low concentrations of template and its implications for quantitative RT-PCR.

21 June 2018

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Thermostabilization and thermoactivation of thermolabile enzymes by trehalose and its application for the synthesis of full length cDNA.

21 June 2018

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Interference of humic acids and DNA extracted directly from soil in detection and transformation of recombinant DNA from bacteria and a yeast

21 June 2018

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Crystal structure of a replication fork single-stranded DNA binding protein (T4 gp32) complexed to DNA

21 June 2018

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RNA chaperones and the RNA folding problem

21 June 2018

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First glimpses at structure-function relationships of the nucleocapsid protein of retroviruses

21 June 2018

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An improved double stranded DNA sequencing method using gene 32 protein.

21 June 2018

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Improved yields of long PCR products using gene 32 protein

21 June 2018

1 reference

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Protein-protein interactions with the acidic COOH terminus of the single-stranded DNA-binding protein of the bacteriophage T4.

21 June 2018

1 reference

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Purification of the T4 gene 32 protein free from detectable deoxyribonuclease activities

21 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2291727

Recent excitement in the DNA replication problem

21 June 2018

1 reference

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Background priming during reverse transcription by oligo(dT) carried over from mRNA isolation.

21 June 2018

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Increased yield of PCR products by addition of T4 gene 32 protein to the SMART PCR cDNA synthesis system

31 October 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2291727

Characterization of RNA strand displacement synthesis by Moloney murine leukemia virus reverse transcriptase

31 October 2018

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Effects of the T4 bacteriophage gene 32 product on the efficiency and fidelity of DNA amplification using T4 DNA polymerase

31 October 2018

1 reference

12 December 2020

Characterization by electron microscopy of the complex formed between T4 bacteriophage gene 32-protein and DNA

1 reference

12 December 2020

Nucleic-acid-chaperone activity of retroviral nucleocapsid proteins: significance for viral replication

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12 December 2020

Products of bacteriophage T4 genes 32 and 45 improve the accuracy of DNA replication in vitro

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12 December 2020

Identifiers

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:16461948%20AND%20SRC:MED&resulttype=core&format=json

16 January 2020

PubMed publication ID

16461948

1 reference