Application of highly sensitive fluorescent dyes (CyDye DIGE Fluor saturation dyes) to laser microdissection and two-dimensional difference gel electrophoresis (2D-DIGE) for cancer proteomics (Q33280979)

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English	Application of highly sensitive fluorescent dyes (CyDye DIGE Fluor saturation dyes) to laser microdissection and two-dimensional difference gel electrophoresis (2D-DIGE) for cancer proteomics	scientific article

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scholarly article

1 reference

Europe PubMed Central

25 July 2017

Application of highly sensitive fluorescent dyes (CyDye DIGE Fluor saturation dyes) to laser microdissection and two-dimensional difference gel electrophoresis (2D-DIGE) for cancer proteomics (English)

1 reference

Europe PubMed Central

25 July 2017

electrophoresis

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microdissection

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based on heuristic

inferred from title

Setsuo Hirohashi

2

object named as

Setsuo Hirohashi

1 reference

Europe PubMed Central

25 July 2017

author name string

Tadashi Kondo

1

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Europe PubMed Central

25 July 2017

publication date

1 January 2006

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Europe PubMed Central

25 July 2017

Nature Protocols

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Europe PubMed Central

25 July 2017

1

1 reference

Europe PubMed Central

25 July 2017

6

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Europe PubMed Central

25 July 2017

2940-2956

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Europe PubMed Central

25 July 2017

https://scigraph.springernature.com/pub.10.1038/nprot.2006.421

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Laser capture microdissection and two-dimensional polyacrylamide gel electrophoresis: evaluation of tissue preparation and sample limitations

1 reference

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21 January 2018

Laser-capture microdissection

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Use of immuno-LCM to identify the in situ expression profile of cellular constituents of the tumor microenvironment

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The potential use of laser capture microdissection to selectively obtain distinct populations of cells for proteomic analysis--preliminary findings.

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Current two-dimensional electrophoresis technology for proteomics

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State-of-the-art two-dimensional gel electrophoresis: a key tool of proteomics research

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A database of protein expression in lung cancer

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35S-methionine labelled polypeptides from HeLa cells, coordinates and percentage of some major polypeptides

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Differential radioactive proteomic analysis of microdissected renal cell carcinoma tissue by 54 cm isoelectric focusing in serial immobilized pH gradient gels

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Application of sensitive fluorescent dyes in linkage of laser microdissection and two-dimensional gel electrophoresis as a cancer proteomic study tool

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Difference gel electrophoresis: a single gel method for detecting changes in protein extracts

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Evaluation of saturation labelling two-dimensional difference gel electrophoresis fluorescent dyes

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2D differential in-gel electrophoresis for the identification of esophageal scans cell cancer-specific protein markers

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A novel experimental design for comparative two-dimensional gel analysis: two-dimensional difference gel electrophoresis incorporating a pooled internal standard

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Antibody-based proteomics for human tissue profiling

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Plasma proteomics of lung cancer by a linkage of multi-dimensional liquid chromatography and two-dimensional difference gel electrophoresis

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2-D DIGE analysis of butyrate-treated HCT-116 cells after enrichment with heparin affinity chromatography

1 reference

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Strategies for revealing lower abundance proteins in two-dimensional protein maps.

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Comprehensive proteome analysis by chromatographic protein prefractionation.

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Chromatofocusing fractionation and two-dimensional difference gel electrophoresis for low abundance serum proteins.

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Very-high-resolution two-dimensional electrophoretic separation of proteins on giant gels

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Genetic analysis of the mouse brain proteome.

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Proteomic signatures for histological types of lung cancer

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Proteomic study of human hepatocellular carcinoma using two-dimensional difference gel electrophoresis with saturation cysteine dye.

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Database of two-dimensional polyacrylamide gel electrophoresis of proteins labeled with CyDye DIGE Fluor saturation dye.

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Proteomic signatures corresponding to histological classification and grading of soft-tissue sarcomas

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Protein clusters associated with carcinogenesis, histological differentiation and nodal metastasis in esophageal cancer

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Integrative analysis of the cancer transcriptome

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Protein profiles associated with survival in lung adenocarcinoma

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Saturation labeling with cysteine-reactive cyanine fluorescent dyes provides increased sensitivity for protein expression profiling of laser-microdissected clinical specimens

1 reference

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Comparative proteomic analysis using samples obtained with laser microdissection and saturation dye labelling

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Application of fluorescence difference gel electrophoresis saturation labelling for the analysis of microdissected precursor lesions of pancreatic ductal adenocarcinoma

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Novel approaches to analyse glomerular proteins from smallest scale murine and human samples using DIGE saturation labelling

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Sample application by in-gel rehydration improves the resolution of two-dimensional electrophoresis with immobilized pH gradients in the first dimension

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Improved and simplified in-gel sample application using reswelling of dry immobilized pH gradients

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Cy5 maleimide labelling for sensitive detection of free thiols in native protein extracts: identification of seed proteins targeted by barley thioredoxin h isoforms

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Protein expression pattern distinguishes different lymphoid neoplasms

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Differential protein analysis of spasomolytic polypeptide expressing metaplasia using laser capture microdissection and two-dimensional difference gel electrophoresis.

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7 January 2021

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10.1038/NPROT.2006.421

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Europe PubMed Central

25 July 2017

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Europe PubMed Central

25 July 2017

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