Efficient site-directed saturation mutagenesis using degenerate oligonucleotides. (Q33289038)

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1 January 2020

title

Efficient site-directed saturation mutagenesis using degenerate oligonucleotides (English)

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:17595310%20AND%20SRC:MED&resulttype=core&format=json

1 January 2020

author name string

David L Steffens

series ordinal

1

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:17595310%20AND%20SRC:MED&resulttype=core&format=json

1 January 2020

John G K Williams

series ordinal

2

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:17595310%20AND%20SRC:MED&resulttype=core&format=json

1 January 2020

publication date

1 July 2007

1 reference

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Journal of Biomolecular Techniques

1 January 2020

published in

1 reference

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1 January 2020

volume

18

1 reference

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1 January 2020

issue

3

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1 January 2020

page(s)

147-149

1 reference

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Site-directed mutagenesis using Pfu DNA polymerase and T4 DNA ligase

1 January 2020

cites work

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2062545

An efficient one-step site-directed and site-saturation mutagenesis protocol

21 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2062545

Sequence saturation mutagenesis (SeSaM): a novel method for directed evolution

21 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2062545

Directed evolution of green fluorescent protein by a new versatile PCR strategy for site-directed and semi-random mutagenesis

21 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2062545

Rapid and efficient polymerase chain reaction-based strategies for one-site and two-site mutagenesis.

21 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2062545

An improvement of the site-directed mutagenesis method by combination of megaprimer, one-side PCR and DpnI treatment.

31 October 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2062545

Site-directed mutagenesis by the megaprimer PCR method: variations on a theme for simultaneous introduction of multiple mutations.

31 October 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2062545

Creating random mutagenesis libraries using megaprimer PCR of whole plasmid

31 October 2018

1 reference

PubMed

https://pubmed.ncbi.nlm.nih.gov/17595310

inferred from PubMed ID database lookup

12 December 2020

based on heuristic

Creating randomized amino acid libraries with the QuikChange Multi Site-Directed Mutagenesis Kit

1 reference

PubMed

https://pubmed.ncbi.nlm.nih.gov/17595310

inferred from PubMed ID database lookup

12 December 2020

based on heuristic

A one-tube, two-step polymerase chain reaction-based site-directed mutagenesis method with simple identification of the mutated product

1 reference

PubMed

https://pubmed.ncbi.nlm.nih.gov/17595310

inferred from PubMed ID database lookup

12 December 2020

based on heuristic

Identifiers

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:17595310%20AND%20SRC:MED&resulttype=core&format=json

1 January 2020

PubMed publication ID

17595310

1 reference