Effect of PCR amplicon size on assessments of clone library microbial diversity and community structure (Q33409647)

26 July 2017

title

Effect of PCR amplicon size on assessments of clone library microbial diversity and community structure (English)

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26 July 2017

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26 July 2017

David B Mark Welch

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Julie A. Huber

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Julie A Huber

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1

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Susan M Huse

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3

object named as

Susan M Huse

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26 July 2017

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Mitchell L Sogin

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26 July 2017

Phillip R Neal

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26 July 2017

publication date

9 February 2009

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Environmental Microbiology

26 July 2017

published in

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26 July 2017

volume

11

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26 July 2017

issue

5

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26 July 2017

page(s)

1292-1302

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Microbial population structures in the deep marine biosphere

26 July 2017

cites work

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28 July 2018

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28 July 2018

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New screening software shows that most recent large 16S rRNA gene clone libraries contain chimeras

28 July 2018

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28 July 2018

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At least 1 in 20 16S rRNA sequence records currently held in public repositories is estimated to contain substantial anomalies

28 July 2018

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28 July 2018

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28 July 2018

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28 July 2018

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28 July 2018

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Evaluation of primers and PCR conditions for the analysis of 16S rRNA genes from a natural environment

28 July 2018

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Evaluation of PCR amplification bias by terminal restriction fragment length polymorphism analysis of small-subunit rRNA and mcrA genes by using defined template mixtures of methanogenic pure cultures and soil DNA extracts

28 July 2018

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Heteroduplexes in mixed-template amplifications: formation, consequence and elimination by 'reconditioning PCR'

28 July 2018

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Optimization of annealing temperature to reduce bias caused by a primer mismatch in multitemplate PCR

28 July 2018

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Evaluation of PCR-generated chimeras, mutations, and heteroduplexes with 16S rRNA gene-based cloning

28 July 2018

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PCR bias in ecological analysis: a case study for quantitative Taq nuclease assays in analyses of microbial communities

28 July 2018

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Novel short-fragment PCR assay for highly sensitive broad-spectrum detection of anogenital human papillomaviruses

28 July 2018

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Kinetic bias in estimates of coastal picoplankton community structure obtained by measurements of small-subunit rRNA gene PCR amplicon length heterogeneity

28 July 2018

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Bias in template-to-product ratios in multitemplate PCR

28 July 2018

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28 July 2018

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28 July 2018

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Determination of microbial diversity in environmental samples: pitfalls of PCR-based rRNA analysis

28 July 2018

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Characterization of microbial diversity by determining terminal restriction fragment length polymorphisms of genes encoding 16S rRNA.

28 July 2018

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Bias caused by template annealing in the amplification of mixtures of 16S rRNA genes by PCR

28 July 2018

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Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA

28 July 2018

1 reference

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Effect of genome size and rrn gene copy number on PCR amplification of 16S rRNA genes from a mixture of bacterial species

28 July 2018

1 reference

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Genetic diversity in Sargasso Sea bacterioplankton

28 July 2018

1 reference

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Minimizing deletion mutagenesis artifact during Taq DNA polymerase PCR by E. coli SSB

28 July 2018

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Differential amplification of rRNA genes by polymerase chain reaction

28 July 2018

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28 July 2018

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Effect of primer mismatch, annealing temperature and PCR cycle number on 16S rRNA gene-targetting bacterial community analysis

31 October 2018

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PCR-induced sequence artifacts and bias: insights from comparison of two 16S rRNA clone libraries constructed from the same sample.

31 October 2018

1 reference

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PCR-generated artefact from 16S rRNA gene-specific primers

31 October 2018

1 reference

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10.1111/J.1462-2920.2008.01857.X

31 October 2018

Identifiers

DOI

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26 July 2017

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26 July 2017

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