Optimizing Taq polymerase concentration for improved signal-to-noise in the broad range detection of low abundance bacteria (Q33503239)

27 July 2017

title

Optimizing Taq polymerase concentration for improved signal-to-noise in the broad range detection of low abundance bacteria (English)

1 reference

27 July 2017

Rudolph Spangler

1

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27 July 2017

Noel L Goddard

series ordinal

2

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27 July 2017

David S Thaler

series ordinal

3

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27 July 2017

language of work or name

English

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publication date

15 September 2009

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27 July 2017

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27 July 2017

volume

4

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27 July 2017

issue

9

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27 July 2017

page(s)

e7010

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Creative Commons Attribution 4.0 International

27 July 2017

copyright license

start time

15 September 2009

1 reference

April 2022 Public Data File from Crossref

copyright status

copyrighted

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cites work

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Genomic sequencing of single microbial cells from environmental samples

21 June 2018

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Matching phylogeny and metabolism in the uncultured marine bacteria, one cell at a time

21 June 2018

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Pseudomonas aeruginosa contamination of mouth swabs during production causing a major outbreak

21 June 2018

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Optimization of Real-Time PCR Assay for Rapid and Sensitive Detection of Eubacterial 16S Ribosomal DNA in Platelet Concentrates

21 June 2018

1 reference

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DNase pretreatment of master mix reagents improves the validity of universal 16S rRNA gene PCR results

21 June 2018

1 reference

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Quantitative multiprobe PCR assay for simultaneous detection and identification to species level of bacterial pathogens

21 June 2018

1 reference

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Quantitative real-time PCR for Porphyromonas gingivalis and total bacteria

21 June 2018

1 reference

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Contamination and sensitivity issues with a real-time universal 16S rRNA PCR

21 June 2018

1 reference

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Elimination of bacterial DNA from Taq DNA polymerases by restriction endonuclease digestion.

21 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2737620

Elimination of contaminating DNA within polymerase chain reaction reagents: implications for a general approach to detection of uncultured pathogens.

21 June 2018

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Identification and elimination of DNA sequences in Taq DNA polymerase.

21 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2737620

Use of psoralen as extinguisher of contaminated DNA in PCR.

21 June 2018

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A simple method for elimination of unspecific amplifications in polymerase chain reaction

21 June 2018

1 reference

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Taq polymerase contains bacterial DNA of unknown origin.

21 June 2018

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Tests of the single-hit DNA damage model.

21 January 2018

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Development of broad-range 16S rDNA PCR for use in the routine diagnostic clinical microbiology service.

21 January 2018

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Comparison of different decontamination methods for reagents to detect low concentrations of bacterial 16S DNA by real-time-PCR.

21 January 2018

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Reagent decontamination to eliminate false-positives in Escherichia coli qPCR.

21 January 2018

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Use of UV irradiation to reduce false positivity in polymerase chain reaction.

21 January 2018

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Frequent contamination of Taq polymerase with DNA

31 October 2018

1 reference

12 December 2020

Improving PCR efficiency

1 reference

12 December 2020

Detection of DNA contamination in Taq polymerase

1 reference

12 December 2020

DNase I treatment of Taq DNA polymerase for complete PCR decontamination

1 reference

12 December 2020

Decontamination of polymerase chain reaction reagents for detection of low concentrations of 16S rRNA genes

1 reference

12 December 2020

10.1371/JOURNAL.PONE.0007010

Identifiers

DOI

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27 July 2017

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27 July 2017

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