Merits and limitations of recombinant models for the study of human P450-mediated drug metabolism and toxicity: an intralaboratory comparison. (Q33639797)

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English	Merits and limitations of recombinant models for the study of human P450-mediated drug metabolism and toxicity: an intralaboratory comparison.	scientific article

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8 December 2019

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Merits and limitations of recombinant models for the study of human P450-mediated drug metabolism and toxicity: an intralaboratory comparison (English)

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8 December 2019

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8 December 2019

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T Friedberg

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8 December 2019

M P Pritchard

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8 December 2019

M Bandera

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8 December 2019

S P Hanlon

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L A McLaughlin

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S Ding

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8 December 2019

B Burchell

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C R Wolf

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1 May 1999

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8 December 2019

Drug Metabolism Reviews

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8 December 2019

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8 December 2019

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523-544

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8 December 2019

Evolution of the P450 gene superfamily: animal-plant 'warfare', molecular drive and human genetic differences in drug oxidation

1 reference

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Molecular enzymology of human liver cytosolic sulfotransferases

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Mammalian Epoxide Hydrases: Inducible Enzymes Catalysing the Inactivation of Carcinogenic and Cytotoxic Metabolites Derived from Aromatic and Olefinic Compounds

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Human cytochromes P450: problems and prospects

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Expression and enzymatic activity of recombinant cytochrome P450 17 alpha-hydroxylase in Escherichia coli

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Expression of modified human cytochrome P450 3A4 in Escherichia coli and purification and reconstitution of the enzyme.

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Expression of modified cytochrome P450 2C10 (2C9) in Escherichia coli, purification, and reconstitution of catalytic activity.

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Coexpression of a human P450 (CYP3A4) and P450 reductase generates a highly functional monooxygenase system in Escherichia coli.

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21 January 2018

Drug metabolism by Escherichia coli expressing human cytochromes P450.

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High-level expression in Escherichia coli of enzymatically active fusion proteins containing the domains of mammalian cytochromes P450 and NADPH-P450 reductase flavoprotein

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Synthesis of functional mouse cytochromes P-450 P1 and chimeric P-450 P3-1 in the yeast Saccharomyces cerevisiae.

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21 January 2018

Production of cytochrome P450 reductase yeast-rat hybrid proteins in Saccharomyces cerevisiae

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21 January 2018

Yeast expression of animal and plant P450s in optimized redox environments

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21 January 2018

Expression of mammalian cytochrome P450 using vaccinia virus

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10 Heterologous expression of mammalian P450 in COS cells

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13 Expression of cytochrome P450 cDNAs in human B lymphoblastoid cells: Applications to toxicology and metabolite analysis

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Stable expression of rat cytochrome P-450IIB1 cDNA in Chinese hamster cells (V79) and metabolic activation of aflatoxin B1.

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21 January 2018

V79 Chinese hamster cells genetically engineered for cytochrome P450 and their use in mutagenicity and metabolism studies

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Human cell lines, derived from AHH-1 TK+/- human lymphoblasts, genetically engineered for expression of cytochromes P450.

1 reference

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Selection and coamplification of heterologous genes in mammalian cells

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A general strategy for the expression of recombinant human cytochrome P450s in Escherichia coli using bacterial signal peptides: expression of CYP3A4, CYP2A6, and CYP2E1.

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Functional high level expression of cytochrome P450 CYP2D6 using baculoviral expression systems.

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CYP3A4 expressed by insect cells infected with a recombinant baculovirus containing both CYP3A4 and human NADPH-cytochrome P450 reductase is catalytically similar to human liver microsomal CYP3A4.

1 reference

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21 January 2018

Recombinant yeast in drug metabolism.

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Expression of xenobiotic metabolizing enzymes in breast cancer.

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21 January 2018

Relative expression of cytochrome P450 isoenzymes in human liver and association with the metabolism of drugs and xenobiotics

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21 January 2018

Control of topology and mode of assembly of a polytopic membrane protein by positively charged residues

1 reference

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21 January 2018

Expression of heterologous proteins in Escherichia coli

1 reference

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21 January 2018

Functional co-expression of CYP2D6 and human NADPH-cytochrome P450 reductase in Escherichia coli

1 reference

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21 January 2018

Coexpression of mammalian cytochrome P450 and reductase in Escherichia coli

1 reference

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Heterologous gene expression in yeast

1 reference

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Vectors used for expression in mammalian cells

1 reference

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Identifiers

10.1081/DMR-100101934

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Europe PubMed Central

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https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:10335451%20AND%20SRC:MED&resulttype=core&format=json

8 December 2019

PubMed publication ID

1 reference

Europe PubMed Central

PubMed publication ID

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:10335451%20AND%20SRC:MED&resulttype=core&format=json

8 December 2019

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