Archaeal dUTPase enhances PCR amplifications with archaeal DNA polymerases by preventing dUTP incorporation (Q33894188)

29 July 2017

Archaeal dUTPase enhances PCR amplifications with archaeal DNA polymerases by preventing dUTP incorporation (English)

1 reference

29 July 2017

Holly H Hogrefe

1

1 reference

29 July 2017

Connie J Hansen

series ordinal

2

1 reference

29 July 2017

Bradley R Scott

series ordinal

3

1 reference

29 July 2017

Kirk B Nielson

series ordinal

4

1 reference

29 July 2017

language of work or name

English

0 references

publication date

8 January 2002

1 reference

Proceedings of the National Academy of Sciences of the United States of America

29 July 2017

1 reference

29 July 2017

99

1 reference

29 July 2017

2

1 reference

29 July 2017

596-601

1 reference

Molecular characterization of lantibiotic-synthesizing enzyme EpiD reveals a function for bacterial Dfp proteins in coenzyme A biosynthesis

29 July 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=117351

Characterization of a thermostable DNA glycosylase specific for U/G and T/G mismatches from the hyperthermophilic archaeon Pyrobaculum aerophilum.

28 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=117351

Functional interactions of a homolog of proliferating cell nuclear antigen with DNA polymerases in Archaea.

28 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=117351

A read-ahead function in archaeal DNA polymerases detects promutagenic template-strand uracil.

28 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=117351

Primer specific and mispair extension analysis (PSMEA) as a simple approach to fast genotyping

28 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=117351

Biochemical and phylogenetic characterization of the dUTPase from the archaeal virus SIRV.

28 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=117351

Complete genome sequence of Methanobacterium thermoautotrophicum deltaH: functional analysis and comparative genomics

28 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=117351

Fidelity and mutational spectrum of Pfu DNA polymerase on a human mitochondrial DNA sequence

28 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=117351

Counteracting the mutagenic effect of hydrolytic deamination of DNA 5-methylcytosine residues at high temperature: DNA mismatch N-glycosylase Mig.Mth of the thermophilic archaeon Methanobacterium thermoautotrophicum THF

28 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=117351

A new class of uracil-DNA glycosylases related to human thymine-DNA glycosylase

28 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=117351

PCR fidelity of pfu DNA polymerase and other thermostable DNA polymerases

28 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=117351

A reproducible assay of polymerase chain reaction to detect trinucleotide repeat expansion of Huntington's disease and senile chorea

28 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=117351

Complete Genome Sequence of the Methanogenic Archaeon, Methanococcus jannaschii

28 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=117351

Archaebacterial DNA polymerases tightly bind uracil-containing DNA.

28 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=117351

Crystal structure of the Escherichia coli dUTPase in complex with a substrate analogue (dUDP)

28 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=117351

The purification of a mismatch-specific thymine-DNA glycosylase from HeLa cells

28 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=117351

dUTP pyrophosphatase is an essential enzyme in Saccharomyces cerevisiae

28 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=117351

PCR amplification of up to 35-kb DNA with high fidelity and high yield from lambda bacteriophage templates

28 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=117351

A rapid PCR fidelity assay

28 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=117351

Polishing with T4 or Pfu polymerase increases the efficiency of cloning of PCR fragments

28 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=117351

Sliding clamps of DNA polymerases

28 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=117351

Primary structure of human deoxycytidylate deaminase and overexpression of its functional protein in Escherichia coli

28 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=117351

An N -Glycosidase from Escherichia coli That Releases Free Uracil from DNA Containing Deaminated Cytosine Residues

28 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=117351

beta-Alanine auxotrophy associated with dfp, a locus affecting DNA synthesis in Escherichia coli

28 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=117351

dfp Gene of Escherichia coli K-12, a locus affecting DNA synthesis, codes for a flavoprotein

28 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=117351

Lethality of a dut (deoxyuridine triphosphatase) mutation in Escherichia coli

28 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=117351

Initiation of eukaryotic DNA replication in vitro

28 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=117351

Ligation-independent cloning of PCR products (LIC-PCR)

28 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=117351

Improved yields of long PCR products using gene 32 protein

28 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=117351

Phosphorothioate primers improve the amplification of DNA sequences by DNA polymerases with proofreading activity

28 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=117351

dcd (dCTP deaminase) gene of Escherichia coli: mapping, cloning, sequencing, and identification as a locus of suppressors of lethal dut (dUTPase) mutations

28 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=117351

Distinct subsets of retroviruses encode dUTPase

28 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=117351

Bacteriophage T7 Deoxyribonucleic acid replication in vitro. A protein of Escherichia coli required for bacteriophage T7 DNA polymerase activity

28 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=117351

A DNA polymerase III holoenzyme-like subassembly from an extreme thermophilic eubacterium

28 July 2018

1 reference

https://api.crossref.org/works/10.1073%2FPNAS.012372799

Fidelity and predominant mutations produced by deep vent wild-type and exonuclease-deficient DNA polymerases during in vitro DNA amplification

21 January 2018

1 reference

https://api.crossref.org/works/10.1073%2FPNAS.012372799

Cloning and expression in Escherichia coli of the recombinant his-tagged DNA polymerases from Pyrococcus furiosus and Pyrococcus woesei.

21 January 2018

1 reference

https://api.crossref.org/works/10.1073%2FPNAS.012372799

Uracil-DNA glycosylase in the extreme thermophile Archaeoglobus fulgidus

21 January 2018

1 reference

https://api.crossref.org/works/10.1073%2FPNAS.012372799

Ribonucleotide reductase in the archaeon Pyrococcus furiosus: a critical enzyme in the evolution of DNA genomes?

21 January 2018

1 reference

https://api.crossref.org/works/10.1073%2FPNAS.012372799

Low Incorporation of dUMP by Some Thermostable DNA Polymerases May Limit Their Use in PCR Amplifications

21 January 2018

1 reference

https://api.crossref.org/works/10.1073%2FPNAS.012372799

Heat-induced deamination of cytosine residues in deoxyribonucleic acid

21 January 2018

1 reference

PubMed

https://pubmed.ncbi.nlm.nih.gov/11782527

inferred from PubMed ID database lookup

12 December 2020

based on heuristic

Two-stage PCR protocol allowing introduction of multiple mutations, deletions and insertions using QuikChange Site-Directed Mutagenesis

1 reference

PubMed

https://pubmed.ncbi.nlm.nih.gov/11782527

inferred from PubMed ID database lookup

12 December 2020

based on heuristic

Robust amplification and ethidium-visible detection of the fragile X syndrome CGG repeat using Pfu polymerase

1 reference

PubMed

https://pubmed.ncbi.nlm.nih.gov/11782527

inferred from PubMed ID database lookup

12 December 2020

based on heuristic

Identifiers

DOI

10.1073/PNAS.012372799

1 reference

29 July 2017

0 references

1 reference

29 July 2017

1 reference