Use of the Strep-Tag and streptavidin for detection and purification of recombinant proteins (Q33921941)

30 July 2017

Use of the Strep-Tag and streptavidin for detection and purification of recombinant proteins (English)

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30 July 2017

Skerra A

1

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PubMed ID

11036648

30 July 2017

Schmidt TG

series ordinal

2

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PubMed ID

11036648

30 July 2017

language of work or name

English

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publication date

1 January 2000

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30 July 2017

Methods in Enzymology

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30 July 2017

326

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30 July 2017

271-304

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The random peptide library-assisted engineering of a C-terminal affinity peptide, useful for the detection and purification of a functional Ig Fv fragment.

30 July 2017

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2800%2926060-6

7 January 2021

One-step affinity purification of bacterially produced proteins by means of the "Strep tag" and immobilized recombinant core streptavidin.

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2800%2926060-6

7 January 2021

Molecular interaction between the Strep-tag affinity peptide and its cognate target, streptavidin

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2800%2926060-6

7 January 2021

Mutagenesis of a flexible loop in streptavidin leads to higher affinity for the Strep-tag II peptide and improved performance in recombinant protein purification.

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2800%2926060-6

7 January 2021

Applications of a peptide ligand for streptavidin: the Strep-tag.

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2800%2926060-6

7 January 2021

Use of the tetracycline promoter for the tightly regulated production of a murine antibody fragment in Escherichia coli

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2800%2926060-6

7 January 2021

Availability of the fibre subunit CsgA and the nucleator protein CsgB during assembly of fibronectin-binding curli is limited by the intracellular concentration of the novel lipoprotein CsgG.

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2800%2926060-6

7 January 2021

A RecombinantEscherichia coliSensor Strain for the Detection of Tetracyclines

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2800%2926060-6

7 January 2021

Fermenter production of an artificial fab fragment, rationally designed for the antigen cystatin, and its optimized crystallization through constant domain shuffling.

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2800%2926060-6

7 January 2021

The Escherichia coli-derived Fab fragment of the IgM/kappa antibody IN-1 recognizes and neutralizes myelin-associated inhibitors of neurite growth

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2800%2926060-6

7 January 2021

Assembly of a functional immunoglobulin Fv fragment in Escherichia coli

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2800%2926060-6

7 January 2021

The disulfide bonds in antibody variable domains: effects on stability, folding in vitro, and functional expression in Escherichia coli.

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2800%2926060-6

7 January 2021

Protein folding in the periplasm of Escherichia coli

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2800%2926060-6

7 January 2021

Functional expression of the uncomplexed serum retinol-binding protein in Escherichia coli. Ligand binding and reversible unfolding characteristics.

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2800%2926060-6

7 January 2021

Secretion and in vivo folding of the Fab fragment of the antibody McPC603 in Escherichia coli: influence of disulphides and cis-prolines

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2800%2926060-6

7 January 2021

Glutathione S-transferase can be used as a C-terminal, enzymatically active dimerization module for a recombinant protease inhibitor, and functionally secreted into the periplasm of Escherichia coli

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2800%2926060-6

7 January 2021

Rapid and efficient site-specific mutagenesis without phenotypic selection

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2800%2926060-6

7 January 2021

Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2800%2926060-6

7 January 2021

Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2800%2926060-6

7 January 2021

[34] Expression of functional antibody Fv and Fab fragments in Escherichia coli

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2800%2926060-6

7 January 2021

Release of outer membrane fragments from wild-type Escherichia coli and from several E. coli lipopolysaccharide mutants by EDTA and heat shock treatments

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2800%2926060-6

7 January 2021

Leakage of periplasmic proteins from Escherichia coli mediated by polymyxin B nonapeptide

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2800%2926060-6

7 January 2021

Understanding, improving and using green fluorescent proteins

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2800%2926060-6

7 January 2021

The pRSET family of T7 promoter expression vectors for Escherichia coli.

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2800%2926060-6

7 January 2021

Crystal structure of Pseudomonas aeruginosa apo-azurin at 1.85 A resolution

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2800%2926060-6

7 January 2021

A new human selenium-containing protein. Purification, characterization, and cDNA sequence

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2800%2926060-6

7 January 2021

Peptide and protein molecular weight determination by electrophoresis using a high-molarity tris buffer system without urea

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2800%2926060-6

7 January 2021

A rapid, sensitive method for detection of alkaline phosphatase-conjugated anti-antibody on Western blots.

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2800%2926060-6

7 January 2021

Engineered Fv fragments as a tool for the one-step purification of integral multisubunit membrane protein complexes.

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2800%2926060-6

7 January 2021

Mammalian DNA ligases

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2800%2926060-6

7 January 2021

Activity of DNA ligase IV stimulated by complex formation with XRCC4 protein in mammalian cells

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2800%2926060-6

7 January 2021

10.1016/S0076-6879(00)26060-6

Identifiers

DOI

1 reference

30 July 2017

1 reference

PubMed ID

11036648