Immunoblotting, dot-blotting, and ELISPOT assays: methods and applications (Q33993615)

30 July 2017

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title

Immunoblotting, dot-blotting, and ELISPOT assays: methods and applications (English)

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30 July 2017

Stott DI

1

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30 July 2017

publication date

1 May 2000

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30 July 2017

published in

Journal of Immunoassay

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30 July 2017

volume

21

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30 July 2017

issue

2-3

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30 July 2017

page(s)

273-296

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"Golden blot"--detection of polyclonal and monoclonal antibodies bound to antigens on nitrocellulose by protein A-gold complexes

30 July 2017

cites work

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Nondenaturing protein electrotransfer of the esterase activity of lipolytic preparations

21 January 2018

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Mapping of radiolabeled peptides derived from proteolysis of polypeptides bound to nitrocellulose after "Western" blotting.

21 January 2018

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Detection of human cytokine-secreting cells in distinct anatomical compartments

21 January 2018

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Multiple successive immunoprinting: a fast blotting technique of a single agarose isoelectric focusing gel.

21 January 2018

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Characterization of N-linked oligosaccharides by affinoblotting with concanavalin A-peroxidase and treatment of the blots with glycosidases

21 January 2018

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Naturally occurring antibodies to bovine and human retinal S antigen: a comparison between uveitis patients and healthy volunteers

21 January 2018

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Protein and cell membrane iodinations with a sparingly soluble chloroamide, 1,3,4,6-tetrachloro-3a,6a-diphenylglycoluril

21 January 2018

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Protein blotting in uniform or gradient electric fields.

21 January 2018

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India ink staining of proteins on nitrocellulose paper.

21 January 2018

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Quantitative dot-blot assay for proteins using enhanced chemiluminescence

21 January 2018

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A cleavable biotinylating agent and its use in protein electroblots

21 January 2018

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Intensification of peroxidase-diaminobenzidine staining using gold-sulfide-silver: A rapid and highly sensitive method for visualization in immunoblotting

21 January 2018

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A quantitative dot-immunobinding assay for proteins using nitrocellulose membrane filters

21 January 2018

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Affinity immunoblotting detection of serum monoclonal immunoglobulins reactive with glycosphingolipids

21 January 2018

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The digoxigenin:anti-digoxigenin (DIG) technology--a survey on the concept and realization of a novel bioanalytical indicator system

21 January 2018

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The use of nitrocellulose immunoblots for the analysis of antigen recognition by T lymphocytes.

21 January 2018

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Immunization with Nanogram Quantities of Nitrocellulose-Bound Antigen, Electroblotted from Sodium Dodecyl Sulphate-Polyacrylamide Gels

21 January 2018

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A silver stain for the rapid quantitative detection of proteins or nucleic acids on membranes or thin layer plates.

21 January 2018

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A blocking agent and a blocking step are not needed in ELISA, immunostaining dot-blots and western blots

21 January 2018

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A filter immuno-plaque assay for the detection of antibody-secreting cells in vitro.

21 January 2018

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Immunoblotting techniques with picogram sensitivity in cerebrospinal fluid protein detection

21 January 2018

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An immunoblot assay for the simultaneous quantification of several antigens

21 January 2018

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A luminescence Western blot with enhanced sensitivity for antibodies to human immunodeficiency virus

21 January 2018

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Luminography - a new, highly sensitive visualization method for electrophoresis

21 January 2018

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An improved scheme for the identification of antigens recognized by specific antibodies in two-dimensional gel electrophoresis and immunoblotting.

21 January 2018

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The incidence, clonal origin and secretory nature of serum paraproteins in chronic lymphocytic leukaemia

21 January 2018

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Immunoblotting and dot blotting

21 January 2018

1 reference

https://api.crossref.org/works/10.1080%2F01971520009349537

Spectrotypes of anti-DNA antibodies show that anti-DNA-secreting B-cell clones of SLE patients are restricted in number, stable and long lived

21 January 2018

1 reference

https://api.crossref.org/works/10.1080%2F01971520009349537

Isoelectric focusing and reverse immunoblotting of autoantibodies against high molecular weight antigens

21 January 2018

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https://api.crossref.org/works/10.1080%2F01971520009349537

A rapid procedure for preparing fluorescein-labeled specific antibodies from whole antiserum: its use in analyzing cytoskeletal architecture

21 January 2018

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Use of immunoplot analysis for the identification of immunodominant non-variant antigens of Trypanosoma brucei rhodesiense

21 January 2018

1 reference

https://api.crossref.org/works/10.1080%2F01971520009349537

Electrotransfer of basic proteins from nondenaturing polyacrylamide acid gels to nitrocellulose: Detection of enzymatic and inhibitory activities and retention of protein antigenicity

21 January 2018

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Western blots from sodium dodecyl sulfate-polyacrylamide gels stained by metal salts

21 January 2018

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https://api.crossref.org/works/10.1080%2F01971520009349537

An enhanced chemiluminescence detection system combined with a modified immunoblot technique for the detection of low molecular weight IgM in sera from healthy adults and neonates

21 January 2018

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https://api.crossref.org/works/10.1080%2F01971520009349537

An improved method for the detection of peroxidase-conjugated antibodies on immunoblots

21 January 2018

1 reference

https://api.crossref.org/works/10.1080%2F01971520009349537

Characterization of isoforms of the major allergen Phl p V by two-dimensional immunoblotting and microsequencing.

21 January 2018

1 reference

https://api.crossref.org/works/10.1080%2F01971520009349537

Sensitive colloidal metal (gold or silver) staining of protein blots on nitrocellulose membranes

21 January 2018

1 reference

https://api.crossref.org/works/10.1080%2F01971520009349537

Direct blotting with viable cells of protein mixtures separated by two-dimensional gel electrophoresis

21 January 2018

1 reference

https://api.crossref.org/works/10.1080%2F01971520009349537

A New Method for the Detection of Peptides and Similar Compounds on Paper Chromatograms

21 January 2018

1 reference

https://api.crossref.org/works/10.1080%2F01971520009349537

10.1080/01971520009349537

21 January 2018

Identifiers

DOI

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30 July 2017

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