Protein engineering of cytochromes P-450. (Q34126229)

31 July 2017

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title

Protein engineering of cytochromes P-450. (English)

1 reference

31 July 2017

C S Miles

1

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31 July 2017

T W Ost

2

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31 July 2017

M A Noble

3

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31 July 2017

A W Munro

4

1 reference

31 July 2017

S K Chapman

5

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31 July 2017

language of work or name

English

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publication date

1 December 2000

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Biochimica et Biophysica Acta

31 July 2017

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31 July 2017

1543

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31 July 2017

2

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31 July 2017

383-407

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The P450 superfamily: update on new sequences, gene mapping, accession numbers, early trivial names of enzymes, and nomenclature

31 July 2017

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7 January 2021

Bacterial cytochromes P-450.

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7 January 2021

P450 superfamily: update on new sequences, gene mapping, accession numbers and nomenclature.

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7 January 2021

Cloning of a potential cytochrome P450 from the archaeon Sulfolobus solfataricus

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7 January 2021

Crystal structure of nitric oxide reductase from denitrifying fungus Fusarium oxysporum

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7 January 2021

Active-site topology of bovine cholesterol side-chain cleavage cytochrome P450 (P450scc) and evidence for interaction of tyrosine 94 with the side chain of cholesterol

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7 January 2021

Interaction of polycyclic aromatic hydrocarbons with human cytochrome P450 1A1: a CO flash photolysis study

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7 January 2021

Mammalian microsomal cytochrome P450 monooxygenase: structural adaptations for membrane binding and functional diversity

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7 January 2021

Crystallization and preliminary X-ray diffraction analysis of a cytochrome P450 (CYP119) from Sulfolobus solfataricus

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7 January 2021

Crystal structure of substrate-free Pseudomonas putida cytochrome P-450

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7 January 2021

Crystal structure of hemoprotein domain of P450BM-3, a prototype for microsomal P450's

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7 January 2021

Crystal structure and refinement of cytochrome P450terp at 2.3 A resolution

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7 January 2021

Structure of cytochrome P450eryF involved in erythromycin biosynthesis

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7 January 2021

High-resolution crystal structure of cytochrome P450cam

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7 January 2021

The structure of the cytochrome p450BM-3 haem domain complexed with the fatty acid substrate, palmitoleic acid

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7 January 2021

Crystallization and preliminary X-ray diffraction analysis of cytochrome P450sca-2 from Streptomyces carbophilus involved in production of pravastatin sodium, a tissue-selective inhibitor of HMG-CoA reductase

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7 January 2021

Inhibitor-induced conformational change in cytochrome P-450CAM

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7 January 2021

Role of residue 480 in substrate specificity of cytochrome P450 2B5 and 2B11.

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7 January 2021

A predicted three-dimensional structure of human cytochrome P450: implications for substrate specificity

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7 January 2021

Application of 3-Dimensional Homology Modeling of Cytochrome P450 2B1 for Interpretation of Site-Directed Mutagenesis Results

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7 January 2021

Molecular modeling of the 3-D structure of cytochrome P-450scc

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7 January 2021

Structure and function of cytochromes P450: a comparative analysis of three crystal structures.

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7 January 2021

Molecular modelling of the human cytochrome P450 isoform CYP2A6 and investigations of CYP2A substrate selectivity

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7 January 2021

Molecular modelling of CYP4A subfamily members based on sequence homology with CYP102

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7 January 2021

A three-dimensional model of aromatase cytochrome P450.

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7 January 2021

Site-Directed Mutagenesis as a Tool for Molecular Modeling of Cytochrome P450 2B1

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7 January 2021

Molecular modeling of cytochrome P450 2B1: mode of membrane insertion and substrate specificity

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7 January 2021

Molecular modeling of cytochrome P450 3A4

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7 January 2021

Enzymatic determinants of the substrate specificity of CYP2C9: role of B'-C loop residues in providing the pi-stacking anchor site for warfarin binding

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7 January 2021

Engineering microsomal cytochrome P450 2C5 to be a soluble, monomeric enzyme. Mutations that alter aggregation, phospholipid dependence of catalysis, and membrane binding

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7 January 2021

Molecular recognition in cytochrome P-450: mechanism for the control of uncoupling reactions.

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7 January 2021

The oxidation of naphthalene and pyrene by cytochrome P450cam

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7 January 2021

Cytochrome P-450cam monooxygenase can be redesigned to catalyse the regioselective aromatic hydroxylation of diphenylmethane

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7 January 2021

Engineering the substrate specificity of Bacillus megaterium cytochrome P-450 BM3: hydroxylation of alkyl trimethylammonium compounds

1 reference

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7 January 2021

Roles of key active-site residues in flavocytochrome P450 BM3

1 reference

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7 January 2021

A Single Mutation in Cytochrome P450 BM3 Changes Substrate Orientation in a Catalytic Intermediate and the Regiospecificity of Hydroxylation†

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7 January 2021

The catalytic mechanism of cytochrome P450 BM3 involves a 6 Å movement of the bound substrate on reduction

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7 January 2021

Glu-320 and Asp-323 are determinants of the CYP4A1 hydroxylation regiospecificity and resistance to inactivation by 1-aminobenzotriazole

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7 January 2021

Human liver microsomal steroid metabolism: identification of the major microsomal steroid hormone 6 beta-hydroxylase cytochrome P-450 enzyme

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7 January 2021

Evidence for cytochrome P-450NF, the nifedipine oxidase, being the principal enzyme involved in the bioactivation of aflatoxins in human liver

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7 January 2021

Cooperativity in oxidations catalyzed by cytochrome P450 3A4.

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7 January 2021

Alanine-scanning mutagenesis of a putative substrate recognition site in human cytochrome P450 3A4. Role of residues 210 and 211 in flavonoid activation and substrate specificity

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7 January 2021

Molecular modelling of CYP3A4 from an alignment with CYP102: identification of key interactions between putative active site residues and CYP3A-specific chemicals

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7 January 2021

Analysis of human cytochrome P450 3A4 cooperativity: construction and characterization of a site-directed mutant that displays hyperbolic steroid hydroxylation kinetics

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7 January 2021

Analysis of four residues within substrate recognition site 4 of human cytochrome P450 3A4: role in steroid hydroxylase activity and alpha-naphthoflavone stimulation.

1 reference

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7 January 2021

Identification of three key residues in substrate recognition site 5 of human cytochrome P450 3A4 by cassette and site-directed mutagenesis.

1 reference

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7 January 2021

The importance of SRS-1 residues in catalytic specificity of human cytochrome P450 3A4.

1 reference

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7 January 2021

Structure-function relationships of human liver cytochromes P450 3A: aflatoxin B1 metabolism as a probe

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7 January 2021

Identification by in vitro mutagenesis of the interaction of two segments of C2MstC1, a chimera of cytochromes P450 2C2 and P450 2C1.

1 reference

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7 January 2021

Molecular basis for the differences in lidocaine binding and regioselectivity of oxidation by cytochromes P450 2B1 and 2B2

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7 January 2021

Elucidation of amino acid residues critical for unique activities of rabbit cytochrome P450 2B5 using hybrid enzymes and reciprocal site-directed mutagenesis with rabbit cytochrome P450 2B4.

1 reference

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7 January 2021

Identification of residues 286 and 289 as critical for conferring substrate specificity of human CYP2C9 for diclofenac and ibuprofen

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7 January 2021

Identification of residues 99, 220, and 221 of human cytochrome P450 2C19 as key determinants of omeprazole activity

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7 January 2021

Identification of amino acid substitutions that confer a high affinity for sulfaphenazole binding and a high catalytic efficiency for warfarin metabolism to P450 2C19.

1 reference

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7 January 2021

Use of chimeric enzymes and site-directed mutagenesis for identification of three key residues responsible for differences in steroid hydroxylation between canine cytochromes P-450 3A12 and 3A26

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7 January 2021

Mutual conversion of fatty-acid substrate specificity by a single amino-acid exchange at position 527 in P-450Cm2 and P-450Alk3A

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7 January 2021

Cloning and expression of complementary DNAs for multiple members of the human cytochrome P450IIC subfamily

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7 January 2021

Biochemistry and molecular biology of the human CYP2C subfamily

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7 January 2021

Evidence that CYP2C19 is the major (S)-mephenytoin 4'-hydroxylase in humans

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7 January 2021

Tolbutamide and phenytoin hydroxylations by cDNA-expressed human liver cytochrome P4502C9

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7 January 2021

Interaction of sulfaphenazole derivatives with human liver cytochromes P450 2C: molecular origin of the specific inhibitory effects of sulfaphenazole on CYP 2C9 and consequences for the substrate binding site topology of CYP 2C9

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7 January 2021

Identification of functional domains of adenylyl cyclase using in vivo chimeras

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7 January 2021

Studies of distant members of the P450 superfamily (P450scc and P450c27) by random chimeragenesis.

1 reference

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7 January 2021

The use of random chimeragenesis to study structure/function properties of rat and human P450c17

1 reference

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7 January 2021

Design of a novel P450: a functional bacterial-human cytochrome P450 chimera.

1 reference

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7 January 2021

N-terminal modifications that alter P450 membrane targeting and function

1 reference

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7 January 2021

Determination of membrane-bound fragments of cytochrome P-450 2B4

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7 January 2021

Substitutions in the C-terminal portion of the catalytic domain partially reverse assembly defects introduced by mutations in the N-terminal linker sequence of cytochrome P450 2C2.

1 reference

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7 January 2021

Molecular engineering of microsomal P450 2a-4 to a stable, water-soluble enzyme.

1 reference

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7 January 2021

Generation of a complete, soluble, and catalytically active sterol 14 alpha-demethylase-reductase complex.

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7 January 2021

NADPH-flavodoxin reductase and flavodoxin from Escherichia coli: characteristics as a soluble microsomal P450 reductase.

1 reference

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7 January 2021

Escherichia coli MTC, a human NADPH P450 reductase competent mutagenicity tester strain for the expression of human cytochrome P450 isoforms 1A1, 1A2, 2A6, 3A4, or 3A5: catalytic activities and mutagenicity studies

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7 January 2021

Use of heterologous expression systems to study autoimmune drug-induced hepatitis

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7 January 2021

Microsomal P450 2C3 is expressed as a soluble dimer in Escherichia coli following modification of its N-terminus

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7 January 2021

A thermodynamic model of regulation: modulation of redox equilibria in camphor monoxygenase

1 reference

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7 January 2021

Redox control of the catalytic cycle of flavocytochrome P-450 BM3.

1 reference

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7 January 2021

Putidaredoxin competitively inhibits cytochrome b5-cytochrome P-450cam association: a proposed molecular model for a cytochrome P-450cam electron-transfer complex

1 reference

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7 January 2021

Significant contribution of arginine-112 and its positive charge of Pseudomonas putida cytochrome P-450cam in the electron transport from putidaredoxin

1 reference

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7 January 2021

Genetic variants in the putidaredoxin-cytochrome P-450cam electron-transfer complex: identification of the residue responsible for redox-state-dependent conformers

1 reference

https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900236-3

7 January 2021

Role of Arg112 of cytochrome p450cam in the electron transfer from reduced putidaredoxin. Analyses with site-directed mutants

1 reference

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7 January 2021

Roles of negatively charged surface residues of putidaredoxin in interactions with redox partners in p450cam monooxygenase system.

1 reference

https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900236-3

7 January 2021

A Role of the Putidaredoxin COOH-terminus in P -450 cam (Cytochrome m ) Hydroxylations

1 reference

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7 January 2021

Electron transport in cytochromes P-450 by covalent switching

1 reference

https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900236-3

7 January 2021

The role of tryptophan 97 of cytochrome P450 BM3 from Bacillus megaterium in catalytic function. Evidence against the 'covalent switching' hypothesis of P-450 electron transfer

1 reference

https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900236-3

7 January 2021

Lack of electron transfer from cytochrome b5 in stimulation of catalytic activities of cytochrome P450 3A4. Characterization of a reconstituted cytochrome P450 3A4/NADPH-cytochrome P450 reductase system and studies with apo-cytochrome b5.

1 reference

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7 January 2021

Cytochrome b5 augments the 17,20-lyase activity of human P450c17 without direct electron transfer

1 reference

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7 January 2021

The cytochrome P-450cam binding surface as defined by site-directed mutagenesis and electrostatic modeling

1 reference

https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900236-3

7 January 2021

Identification of the binding site on cytochrome P450 2B4 for cytochrome b5 and cytochrome P450 reductase.

1 reference

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7 January 2021

Structure and characterization of Ectothiorhodospira vacuolata cytochrome b(558), a prokaryotic homologue of cytochrome b(5)

1 reference

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7 January 2021

Domains of the catalytically self-sufficient cytochrome P-450 BM-3. Genetic construction, overexpression, purification and spectroscopic characterization

1 reference

https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900236-3

7 January 2021

The domain architecture of cytochrome P450BM-3.

1 reference

https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900236-3

7 January 2021

Structure of a cytochrome P450-redox partner electron-transfer complex

1 reference

https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900236-3

7 January 2021

Three-dimensional structure of NADPH-cytochrome P450 reductase: prototype for FMN- and FAD-containing enzymes

1 reference

https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900236-3

7 January 2021

Laboratory evolution of peroxide-mediated cytochrome P450 hydroxylation

1 reference

https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900236-3

7 January 2021

Electrocatalytically driven omega-hydroxylation of fatty acids using cytochrome P450 4A1

1 reference

https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900236-3

7 January 2021

Application of electrochemistry for P450-catalyzed reactions

1 reference

https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900236-3

7 January 2021

The interaction of NADPH-P450 reductase with P450: an electrochemical study of the role of the flavin mononucleotide-binding domain.

1 reference

https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900236-3

7 January 2021

Maximizing expression of eukaryotic cytochrome P450s in Escherichia coli

1 reference

https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900236-3

7 January 2021

A genetically engineered P450 monooxygenase: construction of the functional fused enzyme between rat cytochrome P450c and NADPH-cytochrome P450 reductase

1 reference

https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900236-3

7 January 2021

Genetically engineered P450 monooxygenases: construction of bovine P450c17/yeast reductase fused enzymes

1 reference

https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900236-3

7 January 2021

Expression of bovine cytochrome P450c21 and its fused enzymes with yeast NADPH-cytochrome P450 reductase in Saccharomyces cerevisiae

1 reference

https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900236-3

7 January 2021

Expression and enzymatic activity of recombinant cytochrome P450 17 alpha-hydroxylase in Escherichia coli

1 reference

https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900236-3

7 January 2021

High-level expression in Escherichia coli of enzymatically active fusion proteins containing the domains of mammalian cytochromes P450 and NADPH-P450 reductase flavoprotein

1 reference

https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900236-3

7 January 2021

Human cytochrome P450 3A4: enzymatic properties of a purified recombinant fusion protein containing NADPH-P450 reductase

1 reference

https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900236-3

7 January 2021

Construction of plasmids and expression in Escherichia coli of enzymatically active fusion proteins containing the heme-domain of a P450 linked to NADPH-P450 reductase

1 reference

https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900236-3

7 January 2021

Construction of a human cytochrome P450 1A1: rat NADPH-cytochrome P450 reductase fusion protein cDNA and expression in Escherichia coli, purification, and catalytic properties of the enzyme in bacterial cells and after purification.

1 reference

https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900236-3

7 January 2021

The Ah locus: genetic differences in toxicity, cancer, mutation, and birth defects

1 reference

https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900236-3

7 January 2021

Herbicide-resistant tobacco plants expressing the fused enzyme between rat cytochrome P4501A1 (CYP1A1) and yeast NADPH-cytochrome P450 oxidoreductase

1 reference

https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900236-3

7 January 2021

Mutagenesis study of Asp-290 in cytochrome P450 2B11 using a fusion protein with rat NADPH-cytochrome P450 reductase.

1 reference

https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900236-3

7 January 2021

Putidaredoxin reductase-putidaredoxin-cytochrome p450cam triple fusion protein. Construction of a self-sufficient Escherichia coli catalytic system.

1 reference

https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900236-3

7 January 2021

Construction of a P450c27 fusion enzyme: a useful tool for analysis of vitamin D3 25-hydroxylase activity

1 reference

https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900236-3

7 January 2021

Construction and function of fusion enzymes of the human cytochrome P450scc system

1 reference

https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900236-3

7 January 2021

Construction and characterization of a catalytic fusion protein system: P-450(11beta)-adrenodoxin reductase-adrenodoxin

1 reference

https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900236-3

7 January 2021

Engineering and biochemical characterization of the rat microsomal cytochrome P4501A1 fused to ferredoxin and ferredoxin-NADP(+) reductase from plant chloroplasts

1 reference

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7 January 2021

Molecular engineering study on electron transfer from NADPH-P450 reductase to rat mitochondrial P450c27 in yeast microsomes

1 reference

https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900236-3

7 January 2021

Kinetic studies on a genetically engineered fused enzyme between rat cytochrome P4501A1 and yeast NADPH-P450 reductase.

1 reference

https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900236-3

7 January 2021

MOLSCRIPT: a program to produce both detailed and schematic plots of protein structures

1 reference

https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900236-3

7 January 2021

10.1016/S0167-4838(00)00236-3

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DOI

1 reference

31 July 2017

1 reference