Testing the limits of 454 pyrotag sequencing: reproducibility, quantitative assessment and comparison to T-RFLP fingerprinting of aquifer microbes (Q34342045)

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English	Testing the limits of 454 pyrotag sequencing: reproducibility, quantitative assessment and comparison to T-RFLP fingerprinting of aquifer microbes	scientific article

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scholarly article

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8 February 2020

Testing the limits of 454 pyrotag sequencing: reproducibility, quantitative assessment and comparison to T-RFLP fingerprinting of aquifer microbes (English)

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8 February 2020

reproducibility

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based on heuristic

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Michael S Granitsiotis

2

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8 February 2020

Tillmann Lueders

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8 February 2020

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Giovanni Pilloni

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8 February 2020

Marion Engel

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12 July 2012

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8 February 2020

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8 February 2020

e40467

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Creative Commons Attribution 4.0 International

12 July 2012

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Reducing the effects of PCR amplification and sequencing artifacts on 16S rRNA-based studies

1 reference

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Barcoded primers used in multiplex amplicon pyrosequencing bias amplification

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The evolution of Pyrosequencing® for microbiology: From genes to genomes

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6 July 2018

Generation of multimillion-sequence 16S rRNA gene libraries from complex microbial communities by assembling paired-end illumina reads

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6 July 2018

Mangrove bacterial diversity and the impact of oil contamination revealed by pyrosequencing: bacterial proxies for oil pollution

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6 July 2018

Reproducibility and quantitation of amplicon sequencing-based detection

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6 July 2018

Replicate or lie.

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6 July 2018

Wrinkles in the rare biosphere: pyrosequencing errors can lead to artificial inflation of diversity estimates

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6 July 2018

Metagenomic pyrosequencing and microbial identification

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6 July 2018

The Ribosomal Database Project: improved alignments and new tools for rRNA analysis

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6 July 2018

TRiFLe, a program for in silico terminal restriction fragment length polymorphism analysis with user-defined sequence sets

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6 July 2018

How to get more out of molecular fingerprints: practical tools for microbial ecology

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6 July 2018

Depth-resolved quantification of anaerobic toluene degraders and aquifer microbial community patterns in distinct redox zones of a tar oil contaminant plume.

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6 July 2018

A pyrosequencing-tailored nucleotide barcode design unveils opportunities for large-scale sample multiplexing

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6 July 2018

Naive Bayesian classifier for rapid assignment of rRNA sequences into the new bacterial taxonomy

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6 July 2018

Greengenes, a chimera-checked 16S rRNA gene database and workbench compatible with ARB

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6 July 2018

Formation of pseudo-terminal restriction fragments, a PCR-related bias affecting terminal restriction fragment length polymorphism analysis of microbial community structure

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6 July 2018

Evaluation of PCR amplification bias by terminal restriction fragment length polymorphism analysis of small-subunit rRNA and mcrA genes by using defined template mixtures of methanogenic pure cultures and soil DNA extracts

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6 July 2018

The Interlaboratory RObustness of Next-generation sequencing (IRON) study: a deep sequencing investigation of TET2, CBL and KRAS mutations by an international consortium involving 10 laboratories.

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21 January 2018

Electron acceptor-dependent identification of key anaerobic toluene degraders at a tar-oil-contaminated aquifer by Pyro-SIP.

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The use of stable isotope probing to identify key iron-reducing microorganisms involved in anaerobic benzene degradation

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21 January 2018

High Resolution Analysis of Contaminated Aquifer Sediments and Groundwater—What Can be Learned in Terms of Natural Attenuation?

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21 January 2018

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10.1371/JOURNAL.PONE.0040467

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8 February 2020

2012PLoSO...740467P

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Europe PubMed Central

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:22808168%20AND%20SRC:MED&resulttype=core&format=json

8 February 2020

1 reference

Europe PubMed Central

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8 February 2020

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