Real-time, fluorescence-based quantitative PCR: a snapshot of current procedures and preferences (Q34433461)

2 August 2017

Real-time, fluorescence-based quantitative PCR: a snapshot of current procedures and preferences (English)

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2 August 2017

Stephen A Bustin

1

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publication date

1 July 2005

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Expert Review of Molecular Diagnostics: new diagnostic technologies are set to revolutionise healthcare

2 August 2017

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2 August 2017

5

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2 August 2017

4

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2 August 2017

493-498

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Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays

2 August 2017

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https://api.crossref.org/works/10.1586%2F14737159.5.4.493

Quantification of mRNA using real-time reverse transcription PCR (RT-PCR): trends and problems.

21 January 2018

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https://api.crossref.org/works/10.1586%2F14737159.5.4.493

Gene quantification using real-time quantitative PCR: an emerging technology hits the mainstream

21 January 2018

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https://api.crossref.org/works/10.1586%2F14737159.5.4.493

Real-time PCR in virology

21 January 2018

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https://api.crossref.org/works/10.1586%2F14737159.5.4.493

Improved quantitative real-time RT-PCR for expression profiling of individual cells

21 January 2018

1 reference

https://api.crossref.org/works/10.1586%2F14737159.5.4.493

Quantification using real-time PCR technology: applications and limitations

21 January 2018

1 reference

https://api.crossref.org/works/10.1586%2F14737159.5.4.493

Real-time PCR in the microbiology laboratory

21 January 2018

1 reference

https://api.crossref.org/works/10.1586%2F14737159.5.4.493

Quantitative real-time RT-PCR--a perspective.

21 January 2018

1 reference

https://api.crossref.org/works/10.1586%2F14737159.5.4.493

Intratumor genetic heterogeneity in advanced human colorectal adenocarcinoma.

21 January 2018

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https://api.crossref.org/works/10.1586%2F14737159.5.4.493

Evolution of intratumoral genetic heterogeneity during colorectal cancer progression.

21 January 2018

1 reference

https://api.crossref.org/works/10.1586%2F14737159.5.4.493

Quantification of multiple gene expression in individual cells

21 January 2018

1 reference

https://api.crossref.org/works/10.1586%2F14737159.5.4.493

Clinical utility of viral quantification as a tool for disease monitoring

21 January 2018

1 reference

https://api.crossref.org/works/10.1586%2F14737159.5.4.493

Standardization and quality control studies of 'real-time' quantitative reverse transcriptase polymerase chain reaction of fusion gene transcripts for residual disease detection in leukemia - a Europe Against Cancer program.

21 January 2018

1 reference

https://api.crossref.org/works/10.1586%2F14737159.5.4.493

Standardization of preanalytical factors for minimal residual disease analysis in chronic myelogenous leukemia

21 January 2018

1 reference

https://api.crossref.org/works/10.1586%2F14737159.5.4.493

A third base pair for the polymerase chain reaction: inserting isoC and isoG

21 January 2018

1 reference

https://api.crossref.org/works/10.1586%2F14737159.5.4.493

Nucleic acid analysis using an expanded genetic alphabet to quench fluorescence.

21 January 2018

1 reference

https://api.crossref.org/works/10.1586%2F14737159.5.4.493

Comparison of reverse transcriptases in gene expression analysis.

21 January 2018

1 reference

https://api.crossref.org/works/10.1586%2F14737159.5.4.493

Comparison of amplification enzymes for Hepatitis C Virus quasispecies analysis

21 January 2018

1 reference

https://api.crossref.org/works/10.1586%2F14737159.5.4.493

Properties of the reverse transcription reaction in mRNA quantification

21 January 2018

1 reference

https://api.crossref.org/works/10.1586%2F14737159.5.4.493

Quantitative real-time reverse transcription polymerase chain reaction: normalization to rRNA or single housekeeping genes is inappropriate for human tissue biopsies.

21 January 2018

1 reference

https://api.crossref.org/works/10.1586%2F14737159.5.4.493

Utility of the housekeeping genes 18S rRNA, beta-actin and glyceraldehyde-3-phosphate-dehydrogenase for normalization in real-time quantitative reverse transcriptase-polymerase chain reaction analysis of gene expression in human T lymphocytes

21 January 2018

1 reference

https://api.crossref.org/works/10.1586%2F14737159.5.4.493

Normalization of real-time quantitative reverse transcription-PCR data: a model-based variance estimation approach to identify genes suited for normalization, applied to bladder and colon cancer data sets

21 January 2018

1 reference

https://api.crossref.org/works/10.1586%2F14737159.5.4.493

Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes

21 January 2018

1 reference

https://api.crossref.org/works/10.1586%2F14737159.5.4.493

Relative expression software tool (REST©) for group-wise comparison and statistical analysis of relative expression results in real-time PCR

21 January 2018

1 reference

https://api.crossref.org/works/10.1586%2F14737159.5.4.493

A new mathematical model for relative quantification in real-time RT-PCR

21 January 2018

1 reference

https://api.crossref.org/works/10.1586%2F14737159.5.4.493

Real-time RT-PCR normalisation; strategies and considerations.

21 January 2018

1 reference

https://api.crossref.org/works/10.1586%2F14737159.5.4.493

Customized molecular phenotyping by quantitative gene expression and pattern recognition analysis

21 January 2018

1 reference

https://api.crossref.org/works/10.1586%2F14737159.5.4.493

21 January 2018

Identifiers

DOI

10.1586/14737159.5.4.493

1 reference

release_akqtydzdebfaxbpmtcfidwxid4

2 August 2017

1 reference

Fatcat

https://api.fatcat.wiki/v0/release/akqtydzdebfaxbpmtcfidwxid4

mapped directly with Wikidata item

24 November 2022

based on heuristic

PubMed ID

16013967

1 reference

PubMed ID

16013967