Rational identification of new antibacterial drug targets that are essential for viability using a genomics-based approach (Q34774342)

4 August 2017

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title

Rational identification of new antibacterial drug targets that are essential for viability using a genomics-based approach (English)

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4 August 2017

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Alison F Chalker

1

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PubMed ID

12163125

4 August 2017

R Dwayne Lunsford

series ordinal

2

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4 August 2017

1 July 2002

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Pharmacology & Therapeutics

4 August 2017

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4 August 2017

95

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4 August 2017

1

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4 August 2017

1-20

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Systematic identification of essential genes by in vitro mariner mutagenesis

4 August 2017

1 reference

https://api.crossref.org/works/10.1016%2FS0163-7258%2802%2900222-X

7 January 2021

Genomic-sequence comparison of two unrelated isolates of the human gastric pathogen Helicobacter pylori

1 reference

https://api.crossref.org/works/10.1016%2FS0163-7258%2802%2900222-X

7 January 2021

A genome-based approach for the identification of essential bacterial genes.

1 reference

https://api.crossref.org/works/10.1016%2FS0163-7258%2802%2900222-X

7 January 2021

Construction and initial characterization of Escherichia coli strains with few or no intact chromosomal rRNA operons.

1 reference

https://api.crossref.org/works/10.1016%2FS0163-7258%2802%2900222-X

7 January 2021

Proteomics of Mycoplasma genitalium: identification and characterization of unannotated and atypical proteins in a small model genome

1 reference

https://api.crossref.org/works/10.1016%2FS0163-7258%2802%2900222-X

7 January 2021

Differential expression of over 60 chromosomal genes in Escherichia coli by constitutive expression of MarA.

1 reference

https://api.crossref.org/works/10.1016%2FS0163-7258%2802%2900222-X

7 January 2021

Antibiotic resistance: a view from the pharmaceutical industry

1 reference

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7 January 2021

The millennium bugs--the need for and development of new antibacterials

1 reference

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7 January 2021

The complete genome sequence of Escherichia coli K-12

1 reference

https://api.crossref.org/works/10.1016%2FS0163-7258%2802%2900222-X

7 January 2021

Reconstruction of amino acid biosynthesis pathways from the complete genome sequence.

1 reference

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7 January 2021

Concordance analysis of microbial genomes

1 reference

https://api.crossref.org/works/10.1016%2FS0163-7258%2802%2900222-X

7 January 2021

Infectious causes of chronic inflammatory diseases and cancer

1 reference

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7 January 2021

Development of antimicrobial agents in the era of new and reemerging infectious diseases and increasing antibiotic resistance

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7 January 2021

cag, a pathogenicity island of Helicobacter pylori, encodes type I-specific and disease-associated virulence factors

1 reference

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7 January 2021

Analysis and toxic overexpression in Escherichia coli of a staphylococcal gene encoding isoleucyl-tRNA synthetase

1 reference

https://api.crossref.org/works/10.1016%2FS0163-7258%2802%2900222-X

7 January 2021

Systematic identification of selective essential genes in Helicobacter pylori by genome prioritization and allelic replacement mutagenesis

1 reference

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7 January 2021

Changing patterns of infectious disease

1 reference

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7 January 2021

Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence

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7 January 2021

Massive gene decay in the leprosy bacillus

1 reference

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7 January 2021

Introduction of unmarked mutations in the Helicobacter pylori vacA gene with a sucrose sensitivity marker

1 reference

https://api.crossref.org/works/10.1016%2FS0163-7258%2802%2900222-X

7 January 2021

Identification of Salmonella typhi genes expressed within macrophages by selective capture of transcribed sequences (SCOTS).

1 reference

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7 January 2021

The Isolation of Biochemically Deficient Mutants of Bacteria by Means of Penicillin

1 reference

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7 January 2021

Whole genome comparison of Campylobacter jejuni human isolates using a low-cost microarray reveals extensive genetic diversity.

1 reference

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7 January 2021

High efficiency transformation of E. coli by high voltage electroporation

1 reference

https://api.crossref.org/works/10.1016%2FS0163-7258%2802%2900222-X

7 January 2021

Assignment of folds for proteins of unknown function in three microbial genomes.

1 reference

https://api.crossref.org/works/10.1016%2FS0163-7258%2802%2900222-X

7 January 2021

DNA uptake in bacteria

1 reference

https://api.crossref.org/works/10.1016%2FS0163-7258%2802%2900222-X

7 January 2021

Use of the lactococcal nisA promoter to regulate gene expression in gram-positive bacteria: comparison of induction level and promoter strength.

1 reference

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7 January 2021

Regulated ectopic expression and allelic-replacement mutagenesis as a method for gene essentiality testing in Staphylococcus aureus

1 reference

https://api.crossref.org/works/10.1016%2FS0163-7258%2802%2900222-X

7 January 2021

Complete genome sequence of an M1 strain of Streptococcus pyogenes

1 reference

https://api.crossref.org/works/10.1016%2FS0163-7258%2802%2900222-X

7 January 2021

Whole-genome random sequencing and assembly of Haemophilus influenzae Rd

1 reference

https://api.crossref.org/works/10.1016%2FS0163-7258%2802%2900222-X

7 January 2021

The Minimal Gene Complement of Mycoplasma genitalium

1 reference

https://api.crossref.org/works/10.1016%2FS0163-7258%2802%2900222-X

7 January 2021

Genomic sequence of a Lyme disease spirochaete, Borrelia burgdorferi

1 reference

https://api.crossref.org/works/10.1016%2FS0163-7258%2802%2900222-X

7 January 2021

Complete Genome Sequence of Treponema pallidum, the Syphilis Spirochete

1 reference

https://api.crossref.org/works/10.1016%2FS0163-7258%2802%2900222-X

7 January 2021

Analogous enzymes: independent inventions in enzyme evolution

1 reference

https://api.crossref.org/works/10.1016%2FS0163-7258%2802%2900222-X

7 January 2021

Regulated expression of heterologous genes in Bacillus subtilis using the Tn10 encoded tet regulatory elements

1 reference

https://api.crossref.org/works/10.1016%2FS0163-7258%2802%2900222-X

7 January 2021

The complete sequence of the mucosal pathogen Ureaplasma urealyticum

1 reference

https://api.crossref.org/works/10.1016%2FS0163-7258%2802%2900222-X

7 January 2021

Tn5 in vitro transposition

1 reference

https://api.crossref.org/works/10.1016%2FS0163-7258%2802%2900222-X

7 January 2021

The two types of 3-dehydroquinase have distinct structures but catalyze the same overall reaction

1 reference

https://api.crossref.org/works/10.1016%2FS0163-7258%2802%2900222-X

7 January 2021

Identification of Mycobacterium tuberculosis RNAs synthesized in response to phagocytosis by human macrophages by selective capture of transcribed sequences (SCOTS)

1 reference

https://api.crossref.org/works/10.1016%2FS0163-7258%2802%2900222-X

7 January 2021

Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoter

1 reference

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7 January 2021

Recent advances in large-scale transposon mutagenesis

1 reference

https://api.crossref.org/works/10.1016%2FS0163-7258%2802%2900222-X

7 January 2021

Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12.

1 reference

https://api.crossref.org/works/10.1016%2FS0163-7258%2802%2900222-X

7 January 2021

DNA sequence of both chromosomes of the cholera pathogen Vibrio cholerae

1 reference

https://api.crossref.org/works/10.1016%2FS0163-7258%2802%2900222-X

7 January 2021

Control of methicillin-resistant Staphylococcus aureus in the hospital setting

1 reference

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7 January 2021

The ABC of channel regulation

1 reference

https://api.crossref.org/works/10.1016%2FS0163-7258%2802%2900222-X

7 January 2021

Complete sequence analysis of the genome of the bacterium Mycoplasma pneumoniae

1 reference

https://api.crossref.org/works/10.1016%2FS0163-7258%2802%2900222-X

7 January 2021

Dissemination in Japanese hospitals of strains of Staphylococcus aureus heterogeneously resistant to vancomycin

1 reference

https://api.crossref.org/works/10.1016%2FS0163-7258%2802%2900222-X

7 January 2021

Some recent studies bearing on the one gene-one enzyme hypothesis

1 reference

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7 January 2021

Genome of the Bacterium Streptococcus pneumoniae Strain R6

1 reference

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7 January 2021

Global transposon mutagenesis and a minimal Mycoplasma genome

1 reference

https://api.crossref.org/works/10.1016%2FS0163-7258%2802%2900222-X

7 January 2021

Differential genome analysis applied to the species-specific features of Helicobacter pylori

1 reference

https://api.crossref.org/works/10.1016%2FS0163-7258%2802%2900222-X

7 January 2021

An estimation of minimal genome size required for life

1 reference

https://api.crossref.org/works/10.1016%2FS0163-7258%2802%2900222-X

7 January 2021

A method for demonstrating gene essentiality in Staphylococcus aureus.

1 reference

https://api.crossref.org/works/10.1016%2FS0163-7258%2802%2900222-X

7 January 2021

Regulated antisense RNA eliminates alpha-toxin virulence in Staphylococcus aureus infection

1 reference

https://api.crossref.org/works/10.1016%2FS0163-7258%2802%2900222-X

7 January 2021

Identification of critical staphylococcal genes using conditional phenotypes generated by antisense RNA.

1 reference

https://api.crossref.org/works/10.1016%2FS0163-7258%2802%2900222-X

7 January 2021

Construction of mutant strains of Neisseria gonorrhoeae lacking new antibiotic resistance markers using a two gene cassette with positive and negative selection

1 reference

https://api.crossref.org/works/10.1016%2FS0163-7258%2802%2900222-X

7 January 2021

Comparative genomes of Chlamydia pneumoniae and C. trachomatis

1 reference

https://api.crossref.org/works/10.1016%2FS0163-7258%2802%2900222-X

7 January 2021

Drug-resistant Streptococcus pneumoniae infections: clinical importance, drug treatment, and prevention.

1 reference

https://api.crossref.org/works/10.1016%2FS0163-7258%2802%2900222-X

7 January 2021

The complete genome sequence of the gram-positive bacterium Bacillus subtilis

1 reference

https://api.crossref.org/works/10.1016%2FS0163-7258%2802%2900222-X

7 January 2021

Whole genome sequencing of meticillin-resistant Staphylococcus aureus

1 reference

https://api.crossref.org/works/10.1016%2FS0163-7258%2802%2900222-X

7 January 2021

Replica plating and indirect selection of bacterial mutants

1 reference

https://api.crossref.org/works/10.1016%2FS0163-7258%2802%2900222-X

7 January 2021

Integration of staphylococcal phage L54a occurs by site-specific recombination: structural analysis of the attachment sites

1 reference

https://api.crossref.org/works/10.1016%2FS0163-7258%2802%2900222-X

7 January 2021

Identification of a new regulator in Streptococcus pneumoniae linking quorum sensing to competence for genetic transformation

1 reference

https://api.crossref.org/works/10.1016%2FS0163-7258%2802%2900222-X

7 January 2021

Identification of a genomic island present in the majority of pathogenic isolates of Pseudomonas aeruginosa

1 reference

https://api.crossref.org/works/10.1016%2FS0163-7258%2802%2900222-X

7 January 2021

The VISA/GISA problem: therapeutic implications

1 reference

https://api.crossref.org/works/10.1016%2FS0163-7258%2802%2900222-X

7 January 2021

The gene for toxic shock toxin is carried by a family of mobile pathogenicity islands in Staphylococcus aureus.

1 reference

https://api.crossref.org/works/10.1016%2FS0163-7258%2802%2900222-X

7 January 2021

Methods for generating precise deletions and insertions in the genome of wild-type Escherichia coli: application to open reading frame characterization

1 reference

https://api.crossref.org/works/10.1016%2FS0163-7258%2802%2900222-X

7 January 2021

Streptococcal transformation: essential features and applications of a natural gene exchange system

1 reference

https://api.crossref.org/works/10.1016%2FS0163-7258%2802%2900222-X

7 January 2021

Molecular cloning and characterization of scrB, the structural gene for the Streptococcus mutans phosphoenolpyruvate-dependent sucrose phosphotransferase system sucrose-6-phosphate hydrolase

1 reference

https://api.crossref.org/works/10.1016%2FS0163-7258%2802%2900222-X

7 January 2021

comYA, a gene similar to comGA of Bacillus subtilis, is essential for competence-factor-dependent DNA transformation in Streptococcus gordonii

1 reference

https://api.crossref.org/works/10.1016%2FS0163-7258%2802%2900222-X

7 January 2021

A highly conserved repeated DNA element located in the chromosome ofStreptococcus pneumoniae

1 reference

https://api.crossref.org/works/10.1016%2FS0163-7258%2802%2900222-X

7 January 2021

Rapid cloning of specific DNA fragments of Streptococcus pneumoniae by vector integration into the chromosome followed by endonucleolytic excision

1 reference

https://api.crossref.org/works/10.1016%2FS0163-7258%2802%2900222-X

7 January 2021

A minimal gene set for cellular life derived by comparison of complete bacterial genomes

1 reference

https://api.crossref.org/works/10.1016%2FS0163-7258%2802%2900222-X

7 January 2021

Structural basis for the recognition of isoleucyl-adenylate and an antibiotic, mupirocin, by isoleucyl-tRNA synthetase

1 reference

https://api.crossref.org/works/10.1016%2FS0163-7258%2802%2900222-X

7 January 2021

Complementing genomics with proteomics: the membrane subproteome of Pseudomonas aeruginosa PAO1.

1 reference

https://api.crossref.org/works/10.1016%2FS0163-7258%2802%2900222-X

7 January 2021

Recurrent septicemia in an immunocompromised patient due to probiotic strains of Bacillus subtilis

1 reference

https://api.crossref.org/works/10.1016%2FS0163-7258%2802%2900222-X

7 January 2021

Complete DNA sequence of a serogroup A strain of Neisseria meningitidis Z2491

1 reference

https://api.crossref.org/works/10.1016%2FS0163-7258%2802%2900222-X

7 January 2021

The genome sequence of the food-borne pathogen Campylobacter jejuni reveals hypervariable sequences

1 reference

https://api.crossref.org/works/10.1016%2FS0163-7258%2802%2900222-X

7 January 2021

Complete genome sequence of a multiple drug resistant Salmonella enterica serovar Typhi CT18

1 reference

https://api.crossref.org/works/10.1016%2FS0163-7258%2802%2900222-X

7 January 2021

Genome sequence of Yersinia pestis, the causative agent of plague

1 reference

https://api.crossref.org/works/10.1016%2FS0163-7258%2802%2900222-X

7 January 2021

Bacterial fatty-acid biosynthesis: a genomics-driven target for antibacterial drug discovery

1 reference

https://api.crossref.org/works/10.1016%2FS0163-7258%2802%2900222-X

7 January 2021

Generation of unmarked directed mutations in mycobacteria, using sucrose counter-selectable suicide vectors

1 reference

https://api.crossref.org/works/10.1016%2FS0163-7258%2802%2900222-X

7 January 2021

Genome sequence of enterohaemorrhagic Escherichia coli O157:H7

1 reference

https://api.crossref.org/works/10.1016%2FS0163-7258%2802%2900222-X

7 January 2021

The comparative metabolism of the mollicutes (Mycoplasmas): the utility for taxonomic classification and the relationship of putative gene annotation and phylogeny to enzymatic function in the smallest free-living cells.

1 reference

https://api.crossref.org/works/10.1016%2FS0163-7258%2802%2900222-X

7 January 2021

Multidrug-resistant Mycobacterium tuberculosis: molecular perspectives

1 reference

https://api.crossref.org/works/10.1016%2FS0163-7258%2802%2900222-X

7 January 2021

Genome sequences of Chlamydia trachomatis MoPn and Chlamydia pneumoniae AR39.

1 reference

https://api.crossref.org/works/10.1016%2FS0163-7258%2802%2900222-X

7 January 2021

Identification and molecular analysis of PcsB, a protein required for cell wall separation of group B streptococcus

1 reference

https://api.crossref.org/works/10.1016%2FS0163-7258%2802%2900222-X

7 January 2021

Bacterial transcript imaging by hybridization of total RNA to oligonucleotide arrays

1 reference

https://api.crossref.org/works/10.1016%2FS0163-7258%2802%2900222-X

7 January 2021

A whole-genome microarray reveals genetic diversity among Helicobacter pylori strains

1 reference

https://api.crossref.org/works/10.1016%2FS0163-7258%2802%2900222-X

7 January 2021

Large-scale automated isolation of Escherichia coli mutants with thermosensitive DNA replication

1 reference

https://api.crossref.org/works/10.1016%2FS0163-7258%2802%2900222-X

7 January 2021

Generation of large libraries of random mutants in Bacillus subtilis by PCR-based plasmid multimerization

1 reference

https://api.crossref.org/works/10.1016%2FS0163-7258%2802%2900222-X

7 January 2021

Comparison of whole genome sequences of Chlamydia pneumoniae J138 from Japan and CWL029 from USA.

1 reference

https://api.crossref.org/works/10.1016%2FS0163-7258%2802%2900222-X

7 January 2021

Organization and transfer of heterologous chloramphenicol and tetracycline resistance genes in pneumococcus

1 reference

https://api.crossref.org/works/10.1016%2FS0163-7258%2802%2900222-X

7 January 2021

Genome Sequence of an Obligate Intracellular Pathogen of Humans: Chlamydia trachomatis

1 reference

https://api.crossref.org/works/10.1016%2FS0163-7258%2802%2900222-X

7 January 2021

Use of conditionally counterselectable suicide vectors for allelic exchange

1 reference

https://api.crossref.org/works/10.1016%2FS0163-7258%2802%2900222-X

7 January 2021

The construction of a cloning vector designed for gene replacement in Bordetella pertussis.

1 reference

https://api.crossref.org/works/10.1016%2FS0163-7258%2802%2900222-X

7 January 2021

Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen

1 reference

https://api.crossref.org/works/10.1016%2FS0163-7258%2802%2900222-X

7 January 2021

Comparative genome analysis of the pathogenic spirochetes Borrelia burgdorferi and Treponema pallidum

1 reference

https://api.crossref.org/works/10.1016%2FS0163-7258%2802%2900222-X

7 January 2021

Genetic variability within Helicobacter pylori

1 reference

https://api.crossref.org/works/10.1016%2FS0163-7258%2802%2900222-X

7 January 2021

Metabolism and evolution of Haemophilus influenzae deduced from a whole-genome comparison with Escherichia coli

1 reference

https://api.crossref.org/works/10.1016%2FS0163-7258%2802%2900222-X

7 January 2021

Complete Genome Sequence of Neisseria meningitidis Serogroup B Strain MC58

1 reference

https://api.crossref.org/works/10.1016%2FS0163-7258%2802%2900222-X

7 January 2021

Complete Genome Sequence of a Virulent Isolate of Streptococcus pneumoniae

1 reference

https://api.crossref.org/works/10.1016%2FS0163-7258%2802%2900222-X

7 January 2021

A genomic analysis of two-component signal transduction in Streptococcus pneumoniae

1 reference

https://api.crossref.org/works/10.1016%2FS0163-7258%2802%2900222-X

7 January 2021

Antimicrobial resistance mechanisms: what's hot and what's not in respiratory pathogens.

1 reference

https://api.crossref.org/works/10.1016%2FS0163-7258%2802%2900222-X

7 January 2021

A panoramic view of bacterial transcription

1 reference

https://api.crossref.org/works/10.1016%2FS0163-7258%2802%2900222-X

7 January 2021

The complete genome sequence of the gastric pathogen Helicobacter pylori

1 reference

https://api.crossref.org/works/10.1016%2FS0163-7258%2802%2900222-X

7 January 2021

Novel BOX repeat PCR assay for high-resolution typing of Streptococcus pneumoniae strains

1 reference

https://api.crossref.org/works/10.1016%2FS0163-7258%2802%2900222-X

7 January 2021

Identification, evolution, and essentiality of the mevalonate pathway for isopentenyl diphosphate biosynthesis in gram-positive cocci

1 reference

https://api.crossref.org/works/10.1016%2FS0163-7258%2802%2900222-X

7 January 2021

Rapid method for the identification of essential genes in Staphylococcus aureus

1 reference

https://api.crossref.org/works/10.1016%2FS0163-7258%2802%2900222-X

7 January 2021

Use of the Escherichia coli lac repressor and operator to control gene expression in Bacillus subtilis

1 reference

https://api.crossref.org/works/10.1016%2FS0163-7258%2802%2900222-X

7 January 2021

Regulated gene expression in Staphylococcus aureus for identifying conditional lethal phenotypes and antibiotic mode of action

1 reference

https://api.crossref.org/works/10.1016%2FS0163-7258%2802%2900222-X

7 January 2021

Evolution of bacterial pathogenesis

1 reference

https://api.crossref.org/works/10.1016%2FS0163-7258%2802%2900222-X

7 January 2021

10.1016/S0163-7258(02)00222-X

Identifiers

DOI

1 reference

4 August 2017

1 reference

PubMed ID

12163125