COLD-PCR: improving the sensitivity of molecular diagnostics assays (Q35036964)

5 August 2017

title

COLD-PCR: improving the sensitivity of molecular diagnostics assays (English)

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5 August 2017

Coren A Milbury

1

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5 August 2017

Jin Li

series ordinal

2

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5 August 2017

Pingfang Liu

series ordinal

3

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5 August 2017

G Mike Makrigiorgos

series ordinal

4

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5 August 2017

publication date

1 March 2011

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Expert Review of Molecular Diagnostics: new diagnostic technologies are set to revolutionise healthcare

5 August 2017

published in

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5 August 2017

volume

11

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5 August 2017

issue

2

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5 August 2017

page(s)

159-169

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Ice-COLD-PCR enables rapid amplification and robust enrichment for low-abundance unknown DNA mutations

5 August 2017

cites work

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Increased sensitivity of KRAS mutation detection by high-resolution melting analysis of COLD-PCR products

8 July 2018

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The use of COLD-PCR and high-resolution melting analysis improves the limit of detection of KRAS and BRAF mutations in colorectal cancer.

8 July 2018

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Circulating nucleic acids in cancer and pregnancy

8 July 2018

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High throughput screening of normal and neoplastic tissue samples

8 July 2018

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https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3111913

COLD-PCR-enhanced high-resolution melting enables rapid and selective identification of low-level unknown mutations

8 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3111913

Mutational evolution in a lobular breast tumour profiled at single nucleotide resolution

8 July 2018

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Two-round coamplification at lower denaturation temperature-PCR (COLD-PCR)-based sanger sequencing identifies a novel spectrum of low-level mutations in lung adenocarcinoma

8 July 2018

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COLD-PCR: a new platform for highly improved mutation detection in cancer and genetic testing

8 July 2018

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https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3111913

Coamplification at lower denaturation temperature-PCR increases mutation-detection selectivity of TaqMan-based real-time PCR.

8 July 2018

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PCR-based methods for the enrichment of minority alleles and mutations

8 July 2018

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Subclonal phylogenetic structures in cancer revealed by ultra-deep sequencing

8 July 2018

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Circulating mutant DNA to assess tumor dynamics

8 July 2018

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High resolution melting applications for clinical laboratory medicine

8 July 2018

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Cancers exhibit a mutator phenotype: clinical implications

8 July 2018

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Replacing PCR with COLD-PCR enriches variant DNA sequences and redefines the sensitivity of genetic testing

8 July 2018

1 reference

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The genomic landscapes of human breast and colorectal cancers

8 July 2018

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Patterns of somatic mutation in human cancer genomes

8 July 2018

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https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3111913

Cancer as an evolutionary and ecological process

8 July 2018

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https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3111913

Human cancers express a mutator phenotype

8 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3111913

Allelic dilution obscures detection of a biologically significant resistance mutation in EGFR-amplified lung cancer

8 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3111913

Determination of allele frequency in pooled DNA: comparison of three PCR-based methods

8 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3111913

Detection and quantification of mutations in the plasma of patients with colorectal tumors

8 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3111913

Sensitive sequencing method for KRAS mutation detection by Pyrosequencing

8 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3111913

Assessment of two flexible and compatible SNP genotyping platforms: TaqMan SNP Genotyping Assays and the SNPlex Genotyping System

8 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3111913

EGFR mutation and resistance of non-small-cell lung cancer to gefitinib

8 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3111913

Comparison of archival plasma and formalin-fixed paraffin-embedded tissue for genotyping in hepatocellular carcinoma.

8 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3111913

Amplicon melting analysis with labeled primers: a closed-tube method for differentiating homozygotes and heterozygotes

8 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3111913

Multiple mutations and cancer

8 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3111913

Clonality of multifocal urothelial carcinomas: 10 years of molecular genetic studies

8 July 2018

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https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3111913

Laser capture microdissection

8 July 2018

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https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3111913

Thermal denaturation of double-stranded nucleic acids: prediction of temperatures critical for gradient gel electrophoresis and polymerase chain reaction

8 July 2018

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https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3111913

GNAS1 mutations occur more commonly than previously thought in intramuscular myxoma.

8 July 2018

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https://api.crossref.org/works/10.1586%2FERM.10.115

Prospective study of the accuracy of EGFR mutational analysis by high-resolution melting analysis in small samples obtained from patients with non-small cell lung cancer

21 January 2018

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https://api.crossref.org/works/10.1586%2FERM.10.115

COLD PCR HRM: a highly sensitive detection method for IDH1 mutations

21 January 2018

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https://api.crossref.org/works/10.1586%2FERM.10.115

Statistical mechanical simulation of polymeric DNA melting with MELTSIM.

21 January 2018

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https://api.crossref.org/works/10.1586%2FERM.10.115

Rapid and sensitive detection of KRAS mutation after fast-COLD-PCR enrichment and high-resolution melting analysis.

21 January 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3111913

Multiplex amplification coupled with COLD-PCR and high resolution melting enables identification of low-abundance mutations in cancer samples with low DNA content

25 October 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3111913

Genomic Imbalances in Urothelial Cancer: Intratumor Heterogeneity Versus Multifocality

25 October 2018

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https://api.crossref.org/works/10.1586%2FERM.10.115

Mosaicism in tuberous sclerosis as a potential cause of the failure of molecular diagnosis

21 January 2018

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12 December 2020

Sensitive mutation detection in heterogeneous cancer specimens by massively parallel picoliter reactor sequencing

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12 December 2020

Quantitative heteroduplex analysis

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12 December 2020

DNA melting analysis for detection of single nucleotide polymorphisms

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12 December 2020

Identifiers

DOI

10.1586/ERM.10.115

1 reference

5 August 2017

1 reference

5 August 2017

1 reference