Effect of osmotic stress and heat shock in recombinant protein overexpression and crystallization (Q35781971)

10 August 2017

title

Effect of osmotic stress and heat shock in recombinant protein overexpression and crystallization (English)

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10 August 2017

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3

Sung-Hou Kim

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10 August 2017

4

Rosalind Kim

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10 August 2017

1

Natalia Oganesyan

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10 August 2017

Irina Ankoudinova

2

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10 August 2017

publication date

10 October 2006

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Protein Expression and Purification

10 August 2017

published in

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10 August 2017

volume

52

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10 August 2017

issue

2

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10 August 2017

page(s)

280-285

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Native folding of aggregation-prone recombinant proteins in Escherichia coli by osmolytes, plasmid- or benzyl alcohol-overexpressed molecular chaperones

10 August 2017

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1 reference

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12 July 2018

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Soluble expression of recombinant proteins in the cytoplasm of Escherichia coli

12 July 2018

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Recombinant protein folding and misfolding in Escherichia coli

12 July 2018

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12 July 2018

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Tight binding of deoxyribonucleotide triphosphates to human thymidine kinase 2 expressed in Escherichia coli. Purification and partial characterization of its dimeric and tetrameric forms

12 July 2018

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Overexpression of DsbC and DsbG markedly improves soluble and functional expression of single-chain Fv antibodies in Escherichia coli

12 July 2018

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The osmophobic effect: natural selection of a thermodynamic force in protein folding

12 July 2018

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Expression of recombinant zeta-crystallin in Escherichia coli with the help of GroEL/ES and its purification

12 July 2018

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Chaperonin-assisted folding of glutamine synthetase under nonpermissive conditions: off-pathway aggregation propensity does not determine the co-chaperonin requirement

12 July 2018

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Proline inhibits aggregation during protein refolding

12 July 2018

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Recombinant protein expression in Escherichia coli

12 July 2018

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Uptake and synthesis of compatible solutes as microbial stress responses to high-osmolality environments

12 July 2018

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The Hsp70 and Hsp60 chaperone machines

12 July 2018

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Preparation of selenomethionyl proteins for phase determination

12 July 2018

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Ligation-independent cloning of PCR products (LIC-PCR)

12 July 2018

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Increase of soluble expression in Escherichia coli cytoplasm by a protein disulfide isomerase gene fusion system.

12 July 2018

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Expression and purification of histidine-tagged bacteriophage T7 DNA polymerase

25 September 2018

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Crystal structure of the conserved hypothetical protein MPN330 (GI: 1674200) from Mycoplasma pneumoniae

25 September 2018

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Mutational and structural-based analyses of the osmolyte effect on protein stability

25 September 2018

1 reference

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DnaK and DnaJ facilitated the folding process and reduced inclusion body formation of magnesium transporter CorA overexpressed in Escherichia coli.

25 September 2018

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Chemical chaperones regulate molecular chaperones in vitro and in cells under combined salt and heat stresses

25 September 2018

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Manipulating the amyloid-beta aggregation pathway with chemical chaperones.

25 September 2018

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Protein misfolding and inclusion body formation in recombinant Escherichia coli cells overexpressing Heat-shock proteins.

25 September 2018

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Production of Lewis x tetrasaccharides by metabolically engineered Escherichia coli

25 September 2018

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12 December 2020

Enhancement of the solubility of proteins overexpressed in Escherichia coli by heat shock

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12 December 2020

A novel strategy for production of a highly expressed recombinant protein in an active form

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12 December 2020

Protein synthesis inhibitors and ethanol selectively enhance heterologous expression of P450s and related proteins in Escherichia coli

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12 December 2020

10.1016/J.PEP.2006.09.015

Identifiers

DOI

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10 August 2017

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10 August 2017

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