Basic principles of real-time quantitative PCR. (Q36099379)

12 August 2017

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title

Basic principles of real-time quantitative PCR. (English)

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12 August 2017

Manit Arya

1

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12 August 2017

Iqbal S Shergill

2

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12 August 2017

Magali Williamson

3

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12 August 2017

Lyndon Gommersall

4

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12 August 2017

Neehar Arya

5

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12 August 2017

Hitendra R H Patel

6

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12 August 2017

1 March 2005

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Expert Review of Molecular Diagnostics: new diagnostic technologies are set to revolutionise healthcare

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12 August 2017

5

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12 August 2017

2

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12 August 2017

209-219

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Kinetic PCR Analysis: Real-time Monitoring of DNA Amplification Reactions

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Quantitative PCR analysis of mitochondrial DNA content in patients with mitochondrial disease.

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Quantification of human immunodeficiency virus type 1 proviral load by a TaqMan real-time PCR assay

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Genotyping of single nucleotide polymorphisms in cytokine genes using real-time PCR allelic discrimination technology

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Short PNA molecular beacons for real-time PCR allelic discrimination of single nucleotide polymorphisms

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Induction of hypervascularity without leakage or inflammation in transgenic mice overexpressing hypoxia-inducible factor-1alpha

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Optimisation and validation of methods to assess single nucleotide polymorphisms (SNPs) in archival histological material

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Detection of gene amplification in intraductal and infiltrating breast cancer by laser-assisted microdissection and quantitative real-time PCR.

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Detection of specific polymerase chain reaction product by utilizing the 5'----3' exonuclease activity of Thermus aquaticus DNA polymerase

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Allelic discrimination by nick-translation PCR with fluorogenic probes

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Detection of nucleic acid hybridization by nonradiative fluorescence resonance energy transfer

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Real time quantitative PCR

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A novel method for real time quantitative RT-PCR.

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Absolute gene expression patterns of thioredoxin and glutaredoxin redox systems in mouse

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Evaluation of a quantitative real-time PCR for the detection of respiratory syncytial virus in pulmonary diseases

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Quantification of plasma Epstein-Barr virus DNA in patients with advanced nasopharyngeal carcinoma

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TaqMan amplification system with an internal positive control for HCV RNA quantitation

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Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays

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Effects of urea infusion on the uterine luminal environment of dairy cows.

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Variable expression of some "housekeeping" genes during human keratinocyte differentiation

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Utility of the housekeeping genes 18S rRNA, beta-actin and glyceraldehyde-3-phosphate-dehydrogenase for normalization in real-time quantitative reverse transcriptase-polymerase chain reaction analysis of gene expression in human T lymphocytes

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Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes

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Product differentiation by analysis of DNA melting curves during the polymerase chain reaction

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Quantitative detection of human immunodeficiency virus type 1 (HIV-1) proviral DNA in peripheral blood mononuclear cells by SYBR green real-time PCR technique

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Quantification of cytokine gene expression using an economical real-time polymerase chain reaction method based on SYBR Green I.

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Quantitative analysis of macrophage inhibitory cytokine-1 (MIC-1) gene expression in human prostatic tissues

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An overview of real-time quantitative PCR: applications to quantify cytokine gene expression

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Gene quantification using real-time quantitative PCR: an emerging technology hits the mainstream

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Detection of single nucleotide polymorphisms in the mannose-binding lectin gene using minor groove binder-DNA probes

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Rapid genotyping of single nucleotide polymorphisms using novel minor groove binding DNA oligonucleotides (MGB probes).

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A novel real-time PCR assay for quantitative analysis of methylated alleles (QAMA): analysis of the retinoblastoma locus

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Detection of minimal residual disease in hematologic malignancies by real-time quantitative PCR: principles, approaches, and laboratory aspects.

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Analysis of human cytomegalovirus DNA in urines of newborns and infants by means of a new ultrarapid real-time PCR-system

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Approaching real-time molecular diagnostics: single-pair fluorescence resonance energy transfer (spFRET) detection for the analysis of low abundant point mutations in K-ras oncogenes

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Multiplex detection of four pathogenic retroviruses using molecular beacons

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Single nucleotide polymorphism detection by combinatorial fluorescence energy transfer tags and biotinylated dideoxynucleotides

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Accurate and rapid analysis of residual disease in patients with CML using specific fluorescent hybridization probes for real time quantitative RT-PCR

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21 January 2018

Identifiers

DOI

10.1586/14737159.5.2.209

1 reference

12 August 2017

1 reference