Segmental isotopic labeling of a 140 kDa dimeric multi-domain protein CheA from Escherichia coli by expressed protein ligation and protein trans-splicing. (Q36117328)

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English	Segmental isotopic labeling of a 140 kDa dimeric multi-domain protein CheA from Escherichia coli by expressed protein ligation and protein trans-splicing.	scientific article

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scholarly article

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6 February 2020

Segmental isotopic labeling of a 140 kDa dimeric multi-domain protein CheA from Escherichia coli by expressed protein ligation and protein trans-splicing (English)

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6 February 2020

isotopic labeling

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Escherichia coli

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based on heuristic

inferred from title

5

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6 February 2020

author name string

Yuichi Minato

1

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6 February 2020

Takumi Ueda

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6 February 2020

Asako Machiyama

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6 February 2020

Ichio Shimada

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28 June 2012

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6 February 2020

Journal of Biomolecular NMR

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6 February 2020

53

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6 February 2020

3

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6 February 2020

191-207

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6 February 2020

Kinetic control of one-pot trans-splicing reactions by using a wild-type and designed split intein

1 reference

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2 October 2017

Core unit of chemotaxis signaling complexes

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2 October 2017

Use of protein trans-splicing to produce active and segmentally (2)H, (15)N labeled mannuronan C5-epimerase AlgE4

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2 October 2017

Branched intermediate formation stimulates peptide bond cleavage in protein splicing

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2 October 2017

Using Structural Information to Change the Phosphotransfer Specificity of a Two-Component Chemotaxis Signalling Complex

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2 October 2017

Recent advances in segmental isotope labeling of proteins: NMR applications to large proteins and glycoproteins

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2 October 2017

Site-specific labeling of proteins with NMR-active unnatural amino acids

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2 October 2017

Segmental isotopic labeling of proteins for nuclear magnetic resonance

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In vivo and in vitro protein ligation by naturally occurring and engineered split DnaE inteins

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2 October 2017

Bacterial chemoreceptors: high-performance signaling in networked arrays

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2 October 2017

Protein ligation: applications in NMR studies of proteins.

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2 October 2017

Improved segmental isotope labeling methods for the NMR study of multidomain or large proteins: application to the RRMs of Npl3p and hnRNP L

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2 October 2017

Reconstruction of the chemotaxis receptor-kinase assembly

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2 October 2017

Optimal isotope labelling for NMR protein structure determinations.

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2 October 2017

Signal transduction in bacterial chemotaxis

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2 October 2017

Structure of the two most C-terminal RNA recognition motifs of PTB using segmental isotope labeling.

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2 October 2017

Crystal structure of the CheA histidine phosphotransfer domain that mediates response regulator phosphorylation in bacterial chemotaxis

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3405243

2 October 2017

Protein splicing and related forms of protein autoprocessing

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2 October 2017

Protein synthesis by native chemical ligation: expanded scope by using straightforward methodology

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https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3405243

2 October 2017

Structure of CheA, a signal-transducing histidine kinase

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3405243

2 October 2017

Chemical ligation of folded recombinant proteins: segmental isotopic labeling of domains for NMR studies

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https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3405243

2 October 2017

Structure and dynamics of a CheY-binding domain of the chemotaxis kinase CheA determined by nuclear magnetic resonance spectroscopy

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2 October 2017

Synthesis of proteins by native chemical ligation

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https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3405243

2 October 2017

Segmental isotopic labeling of the Hsp70 molecular chaperone DnaK using expressed protein ligation

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2 July 2018

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2 July 2018

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2 July 2018

Control of protein splicing by intein fragment reassembly.

1 reference

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2 July 2018

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23 September 2018

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23 September 2018

Segmental isotopic labeling of multi-domain and fusion proteins by protein trans-splicing in vivo and in vitro.

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23 September 2018

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23 September 2018

Intein-based biosynthetic incorporation of unlabeled protein tags into isotopically labeled proteins for NMR studies.

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23 September 2018

Dimerization is required for the activity of the protein histidine kinase CheA that mediates signal transduction in bacterial chemotaxis

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23 September 2018

NMR observation of selected segments in a larger protein: central-segment isotope labeling through intein-mediated ligation

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https://pubmed.ncbi.nlm.nih.gov/22740268

12 December 2020

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The cyclization and polymerization of bacterially expressed proteins using modified self-splicing inteins

1 reference

https://pubmed.ncbi.nlm.nih.gov/22740268

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Purification of proteins fused to either the amino or carboxy terminus of the Mycobacterium xenopi gyrase A intein

1 reference

https://pubmed.ncbi.nlm.nih.gov/22740268

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Improved segmental isotope labeling of proteins and application to a larger protein

1 reference

https://pubmed.ncbi.nlm.nih.gov/22740268

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Conformational change of H+-ATPase beta monomer revealed on segmental isotope labeling NMR spectroscopy

1 reference

https://pubmed.ncbi.nlm.nih.gov/22740268

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Insights into the mechanism and catalysis of the native chemical ligation reaction

1 reference

https://pubmed.ncbi.nlm.nih.gov/22740268

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Single-column purification of free recombinant proteins using a self-cleavable affinity tag derived from a protein splicing element

1 reference

https://pubmed.ncbi.nlm.nih.gov/22740268

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Segmental Isotopic Labelling of a Multidomain Protein by Protein Ligation by Protein Trans-Splicing

1 reference

https://pubmed.ncbi.nlm.nih.gov/22740268

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Expressed protein ligation for a large dimeric protein

1 reference

https://pubmed.ncbi.nlm.nih.gov/22740268

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

A segmental labeling strategy for unambiguous determination of domain-domain interactions of large multi-domain proteins

1 reference

https://pubmed.ncbi.nlm.nih.gov/22740268

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Identifiers

10.1007/S10858-012-9628-3

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6 February 2020

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6 February 2020

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