Methods of changing biopolymer volume fraction and cytoplasmic solute concentrations for in vivo biophysical studies. (Q36944206)

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7 January 2020

review article

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title

Methods of changing biopolymer volume fraction and cytoplasmic solute concentrations for in vivo biophysical studies (English)

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7 January 2020

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1

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7 January 2020

author name string

James C Weisshaar

series ordinal

2

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7 January 2020

M Thomas Record

series ordinal

3

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7 January 2020

language of work or name

English

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publication date

1 January 2007

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7 January 2020

published in

Methods in Enzymology

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7 January 2020

volume

428

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7 January 2020

page(s)

487-504

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Biophysical characterization of changes in amounts and activity of Escherichia coli cell and compartment water and turgor pressure in response to osmotic stress

7 January 2020

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Characterization of the cytoplasm of Escherichia coli K-12 as a function of external osmolarity. Implications for protein-DNA interactions in vivo

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Origins of the osmoprotective properties of betaine and proline in Escherichia coli K-12

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Roles of cytoplasmic osmolytes, water, and crowding in the response of Escherichia coli to osmotic stress: biophysical basis of osmoprotection by glycine betaine

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Accumulation of 3-(N-morpholino)propanesulfonate by osmotically stressed Escherichia coli K-12.

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Transient accumulation of potassium glutamate and its replacement by trehalose during adaptation of growing cells of Escherichia coli K-12 to elevated sodium chloride concentrations

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Cation Transport in Escherichia coli: V. Regulation of cation content

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The exclusion of glycine betaine from anionic biopolymer surface: why glycine betaine is an effective osmoprotectant but also a compatible solute

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Quantitative protein stability measurement in vivo

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Physical nature of bacterial cytoplasm.

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Specific covalent labeling of recombinant protein molecules inside live cells

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Concentration dependence of the self-diffusion of human and Lumbricus terrestris hemoglobin

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Thermodynamic stoichiometries of participation of water, cations and anions in specific and non-specific binding of lac repressor to DNA. Possible thermodynamic origins of the "glutamate effect" on protein-DNA interactions

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Macromolecular diffusion in crowded solutions

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Use of urea and glycine betaine to quantify coupled folding and probe the burial of DNA phosphates in lac repressor-lac operator binding

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Monitoring protein stability and aggregation in vivo by real-time fluorescent labeling

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Inhibition of protein aggregation in vitro and in vivo by a natural osmoprotectant

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Determinants of the translational mobility of a small solute in cell cytoplasm

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Osmotic regulation and the biosynthesis of membrane-derived oligosaccharides in Escherichia coli

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Crowding and confinement effects on protein diffusion in vivo

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Solute probes of conformational changes in open complex (RPo) formation by Escherichia coli RNA polymerase at the lambdaPR promoter: evidence for unmasking of the active site in the isomerization step and for large-scale coupled folding in the subse

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Molecular cloning and expression of a locus (mdoA) implicated in the biosynthesis of membrane-derived oligosaccharides in Escherichia coli

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7 January 2021

Choline-glycine betaine pathway confers a high level of osmotic tolerance in Escherichia coli

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7 January 2021

Replacement of potassium chloride by potassium glutamate dramatically enhances protein-DNA interactions in vitro

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Responses of E. coli to osmotic stress: large changes in amounts of cytoplasmic solutes and water

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Culture medium for enterobacteria

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Identifiers

DOI

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https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:17875435%20AND%20SRC:MED&resulttype=core&format=json

7 January 2020

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