Directed evolution of ligand dependence: small-molecule-activated protein splicing (Q36985743)

17 August 2017

Directed evolution of ligand dependence: small-molecule-activated protein splicing (English)

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17 August 2017

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Allen R Buskirk

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17 August 2017

Yi-Ching Ong

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2

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17 August 2017

Zev J Gartner

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3

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17 August 2017

David R Liu

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4

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17 August 2017

language of work or name

English

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publication date

9 July 2004

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Proceedings of the National Academy of Sciences of the United States of America

17 August 2017

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17 August 2017

101

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17 August 2017

29

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17 August 2017

10505-10510

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Conditional protein splicing: a new tool to control protein structure and function in vitro and in vivo.

17 August 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=489967

Crystal structure of a mini-intein reveals a conserved catalytic module involved in side chain cyclization of asparagine during protein splicing

29 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=489967

Small-molecule switches for zinc finger transcription factors

29 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=489967

Investigating and Engineering Enzymes by Genetic Selection

29 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=489967

Chemical genetic approaches for the elucidation of signaling pathways

29 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=489967

High resolution refinement of β-galactosidase in a new crystal form reveals multiple metal-binding sites and provides a structural basis for α-complementation

29 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=489967

Protein splicing and related forms of protein autoprocessing

29 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=489967

Designing small-molecule switches for protein-protein interactions

29 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=489967

Crystal structure of Escherichia coli PurE, an unusual mutase in the purine biosynthetic pathway

29 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=489967

The Mycobacterium tuberculosis recA intein can be used in an ORFTRAP to select for open reading frames

29 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=489967

The structural basis of estrogen receptor/coactivator recognition and the antagonism of this interaction by tamoxifen

29 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=489967

Different positioning of the ligand-binding domain helix 12 and the F domain of the estrogen receptor accounts for functional differences between agonists and antagonists

29 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=489967

Identification and structural characterization of the ATP/ADP-binding site in the Hsp90 molecular chaperone

29 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=489967

Gene expression from plasmids containing the araBAD promoter at subsaturating inducer concentrations represents mixed populations.

29 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=489967

Steroid receptor interactions with heat shock protein and immunophilin chaperones

29 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=489967

Ligand-activated site-specific recombination in mice

29 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=489967

FACS-optimized mutants of the green fluorescent protein (GFP)

29 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=489967

Crystal structure of the Aequorea victoria green fluorescent protein

29 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=489967

AKR1 encodes a candidate effector of the G beta gamma complex in the Saccharomyces cerevisiae pheromone response pathway and contributes to control of both cell shape and signal transduction

29 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=489967

Regulatable promoters of Saccharomyces cerevisiae: comparison of transcriptional activity and their use for heterologous expression

29 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=489967

Transcriptional activation by tetracyclines in mammalian cells

29 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=489967

Yeast vectors for the controlled expression of heterologous proteins in different genetic backgrounds

29 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=489967

Controlling mammalian gene expression with small molecules

29 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=489967

27 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=489967

Applications of video mixing and digital overlay to neuroethology

27 June 2018

1 reference

Crossref

https://api.crossref.org/works/10.1073%2FPNAS.0402762101

Protein splicing triggered by a small molecule

21 January 2018

1 reference

Crossref

https://api.crossref.org/works/10.1073%2FPNAS.0402762101

An in vivo screening system against protein splicing useful for the isolation of non-splicing mutants or inhibitors of the RecA intein of Mycobacterium tuberculosis

21 January 2018

1 reference

Crossref

https://api.crossref.org/works/10.1073%2FPNAS.0402762101

Molecular dissection of the Mycobacterium tuberculosis RecA intein: design of a minimal intein and of a trans-splicing system involving two intein fragments

21 January 2018

1 reference

12 December 2020

Posttranslational regulation of proteins by fusions to steroid-binding domains

1 reference

12 December 2020

A genetic system yields self-cleaving inteins for bioseparations

1 reference

12 December 2020

Protein splicing-based reconstitution of split green fluorescent protein for monitoring protein-protein interactions in bacteria: improved sensitivity and reduced screening time

1 reference

12 December 2020

Identifiers

DOI

10.1073/PNAS.0402762101

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17 August 2017

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1 reference

17 August 2017

PubMed publication ID

15247421

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