Fusion tails for the recovery and purification of recombinant proteins (Q37064470)

18 August 2017

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title

Fusion tails for the recovery and purification of recombinant proteins (English)

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18 August 2017

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https://api.crossref.org/works/10.1016/1046-5928(91)90057-P

2 December 2022

author name string

C. F. Ford

1

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https://www.ebi.ac.uk/europepmc/webservices/rest/search?resulttype=core&format=json&query=SRC%3AMED%20AND%20EXT_ID%3A1821793

2 December 2022

I. Suominen

2

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?resulttype=core&format=json&query=SRC%3AMED%20AND%20EXT_ID%3A1821793

2 December 2022

C. E. Glatz

3

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?resulttype=core&format=json&query=SRC%3AMED%20AND%20EXT_ID%3A1821793

2 December 2022

language of work or name

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1 April 1991

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full work available at URL

18 August 2017

https://api.elsevier.com/content/article/PII:104659289190057P?httpAccept=text/xml

1 reference

https://api.crossref.org/works/10.1016/1046-5928(91)90057-P

https://api.elsevier.com/content/article/PII:104659289190057P?httpAccept=text/plain

2 December 2022

1 reference

https://api.crossref.org/works/10.1016/1046-5928(91)90057-P

Protein Expression and Purification

2 December 2022

published in

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18 August 2017

2

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18 August 2017

95-107

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18 August 2017

2-3

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The purification of eukaryotic polypeptides synthesized in Escherichia coli

18 August 2017

1

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https://api.crossref.org/works/10.1016/1046-5928(91)90057-P

Engineering proteins for purification

2 December 2022

2

1 reference

https://api.crossref.org/works/10.1016/1046-5928(91)90057-P

Gene fusions for purpose of expression: an introduction

2 December 2022

3

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https://api.crossref.org/works/10.1016/1046-5928(91)90057-P

Protein fusions: bioseparation and application

2 December 2022

5

1 reference

https://api.crossref.org/works/10.1016/1046-5928(91)90057-P

Rapid purification of a cloned gene product by genetic fusion and site-specific proteolysis

2 December 2022

8

1 reference

https://api.crossref.org/works/10.1016/1046-5928(91)90057-P

One-step purification of hybrid proteins which have beta-galactosidase activity

2 December 2022

9

1 reference

https://api.crossref.org/works/10.1016/1046-5928(91)90057-P

Genetic engineering of methionyl-tRNA synthetase: in vitro regeneration of an active synthetase by proteolytic cleavage of a methionyl-tRNA synthetase--beta-galactosidase chimeric protein

2 December 2022

12

1 reference

https://api.crossref.org/works/10.1016/1046-5928(91)90057-P

Erythropoietin-beta-D-galactosidase. The generation, purification and use of a fusion protein.

2 December 2022

13

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https://api.crossref.org/works/10.1016/1046-5928(91)90057-P

Easy identification of cDNA clones

2 December 2022

14

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https://api.crossref.org/works/10.1016/1046-5928(91)90057-P

Synthesis of a wheat storage protein subunit in Escherichia coli using novel expression vectors

2 December 2022

15

1 reference

https://api.crossref.org/works/10.1016/1046-5928(91)90057-P

A plasmid vector system for the expression of a triprotein consisting of beta-galactosidase, a collagenase recognition site and a foreign gene product.

2 December 2022

16

1 reference

https://api.crossref.org/works/10.1016/1046-5928(91)90057-P

The pAX plasmids: new gene-fusion vectors for sequencing, mutagenesis and expression of proteins in Escherichia coli.

2 December 2022

17

1 reference

https://api.crossref.org/works/10.1016/1046-5928(91)90057-P

Parasite antigens expressed in Escherichia coli. A refined approach for epidemiological analysis

2 December 2022

18

1 reference

https://api.crossref.org/works/10.1016/1046-5928(91)90057-P

Expression in Escherichia coli of a chemically synthesized gene for the hormone somatostatin

2 December 2022

19

1 reference

https://api.crossref.org/works/10.1016/1046-5928(91)90057-P

Expression in Escherichia coli of chemically synthesized genes for human insulin

2 December 2022

20

1 reference

https://api.crossref.org/works/10.1016/1046-5928(91)90057-P

Expression of cloned β-endorphin gene sequences by Escherichia coli

2 December 2022

21

1 reference

https://api.crossref.org/works/10.1016/1046-5928(91)90057-P

Overproduction and purification of the Tn10-specified inner membrane tetracycline resistance protein Tet using fusions to beta-galactosidase

2 December 2022

27

1 reference

https://api.crossref.org/works/10.1016/1046-5928(91)90057-P

Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase

2 December 2022

28

1 reference

https://api.crossref.org/works/10.1016/1046-5928(91)90057-P

Eukaryotic proteins expressed in Escherichia coli: An improved thrombin cleavage and purification procedure of fusion proteins with glutathione S-transferase

2 December 2022

29

1 reference

https://api.crossref.org/works/10.1016/1046-5928(91)90057-P

The isolation and characterisation of human atrial natriuretic factor produced as a fusion protein in Escherichia coli.

2 December 2022

31

1 reference

https://api.crossref.org/works/10.1016/1046-5928(91)90057-P

Gene synthesis, expression in Escherichia coli and purification of immunoreactive human insulin-like growth factors I and II. Application of a modified HPLC separation technique for hydrophobic proteins

2 December 2022

32

1 reference

https://api.crossref.org/works/10.1016/1046-5928(91)90057-P

Gene fusion vectors based on the gene for staphylococcal protein A.

2 December 2022

33

1 reference

https://api.crossref.org/works/10.1016/1046-5928(91)90057-P

Expression of human insulin-like growth factor I in bacteria: use of optimized gene fusion vectors to facilitate protein purification.

2 December 2022

36

1 reference

https://api.crossref.org/works/10.1016/1046-5928(91)90057-P

Fusions to staphylococcal protein A

2 December 2022

37

1 reference

https://api.crossref.org/works/10.1016/1046-5928(91)90057-P

Human immunodeficiency viral protease is catalytically active as a fusion protein: characterization of the fusion and native enzymes produced in Escherichia coli

2 December 2022

38

1 reference

https://api.crossref.org/works/10.1016/1046-5928(91)90057-P

Direct and selective binding of an acidic transcriptional activation domain to the TATA-box factor TFIID

2 December 2022

39

1 reference

https://api.crossref.org/works/10.1016/1046-5928(91)90057-P

Dual affinity fusion approach and its use to express recombinant human insulin-like growth factor II

2 December 2022

42

1 reference

https://api.crossref.org/works/10.1016/1046-5928(91)90057-P

Production in Escherichia coli and one-step purification of bifunctional hybrid proteins which bind maltose. Export of the Klenow polymerase into the periplasmic space

2 December 2022

43

1 reference

https://api.crossref.org/works/10.1016/1046-5928(91)90057-P

Vectors that facilitate the expression and purification of foreign peptides in Escherichia coli by fusion to maltose-binding protein

2 December 2022

44

1 reference

https://api.crossref.org/works/10.1016/1046-5928(91)90057-P

Export and purification of a cytoplasmic dimeric protein by fusion to the maltose-binding protein of Escherichia coli

2 December 2022

45

1 reference

https://api.crossref.org/works/10.1016/1046-5928(91)90057-P

An Escherichia coli vector to express and purify foreign proteins by fusion to and separation from maltose-binding protein

2 December 2022

46

1 reference

https://api.crossref.org/works/10.1016/1046-5928(91)90057-P

Adsorption to starch of a beta-galactosidase fusion protein containing the starch-binding region of Aspergillus glucoamylase

2 December 2022

48

1 reference

https://api.crossref.org/works/10.1016/1046-5928(91)90057-P

Improved adsorption to starch of a beta-galactosidase fusion protein containing the starch-binding domain from Aspergillus glucoamylase.

2 December 2022

49

1 reference

https://api.crossref.org/works/10.1016/1046-5928(91)90057-P

Fusion to an endoglucanase allows alkaline phosphatase to bind to cellulose

2 December 2022

50

1 reference

https://api.crossref.org/works/10.1016/1046-5928(91)90057-P

Enzyme immobilization using a cellulose-binding domain: properties of a beta-glucosidase fusion protein

2 December 2022

53

1 reference

https://api.crossref.org/works/10.1016/1046-5928(91)90057-P

Carbohydrate-recognition domains as tools for rapid purification of recombinant eukaryotic proteins

2 December 2022

54

1 reference

https://api.crossref.org/works/10.1016/1046-5928(91)90057-P

Purification of a RAS-responsive adenylyl cyclase complex from Saccharomyces cerevisiae by use of an epitope addition method

2 December 2022

57

1 reference

https://api.crossref.org/works/10.1016/1046-5928(91)90057-P

A Short Polypeptide Marker Sequence Useful for Recombinant Protein Identification and Purification

2 December 2022

58

1 reference

https://api.crossref.org/works/10.1016/1046-5928(91)90057-P

A calcium-dependent antibody for identification and purification of recombinant proteins

2 December 2022

59

1 reference

https://api.crossref.org/works/10.1016/1046-5928(91)90057-P

Chemical synthesis and cloning of a poly(arginine)-coding gene fragment designed to aid polypeptide purification.

2 December 2022

60

1 reference

https://api.crossref.org/works/10.1016/1046-5928(91)90057-P

The functional expression of antibody Fv fragments in Escherichia coli: improved vectors and a generally applicable purification technique.

2 December 2022

64

1 reference

https://api.crossref.org/works/10.1016/1046-5928(91)90057-P

Polyelectrolyte precipitation of beta-galactosidase fusions containing poly-aspartic acid tails

2 December 2022

66

1 reference

https://api.crossref.org/works/10.1016/1046-5928(91)90057-P

Recovery of a charged-fusion protein from cell extracts by polyelectrolyte precipitation.

2 December 2022

67

1 reference

https://api.crossref.org/works/10.1016/1046-5928(91)90057-P

Metal chelate affinity chromatography, a new approach to protein fractionation

2 December 2022

69

1 reference

https://api.crossref.org/works/10.1016/1046-5928(91)90057-P

Protein interactions with immobilized transition metal ions: quantitative evaluations of variations in affinity and binding capacity

2 December 2022

70

1 reference

https://api.crossref.org/works/10.1016/1046-5928(91)90057-P

Genetic Approach to Facilitate Purification of Recombinant Proteins with a Novel Metal Chelate Adsorbent

2 December 2022

71

1 reference

https://api.crossref.org/works/10.1016/1046-5928(91)90057-P

Immobilization and affinity purification of recombinant proteins using histidine peptide fusions

2 December 2022

73

1 reference

https://api.crossref.org/works/10.1016/1046-5928(91)90057-P

Enzyme purification by genetically attached polycysteine and polyphenylalanine affinity tails

2 December 2022

75

1 reference

https://api.crossref.org/works/10.1016/1046-5928(91)90057-P

Differential stability of recombinant human insulin-like growth factor II in Escherichia coli and Staphylococcus aureus.

2 December 2022

78

1 reference

https://api.crossref.org/works/10.1016/1046-5928(91)90057-P

Expression and secretion of human atrial natriuretic alpha-factor in Bacillus subtilis using the subtilisin signal peptide.

2 December 2022

81

1 reference

https://api.crossref.org/works/10.1016/1046-5928(91)90057-P

In vivo degradation of secreted fusion proteins by the Escherichia coli outer membrane protease OmpT.

2 December 2022

82

1 reference

https://api.crossref.org/works/10.1016/1046-5928(91)90057-P

Genetics of proteolysis in Escherichia coli*.

2 December 2022

83

1 reference

https://api.crossref.org/works/10.1016/1046-5928(91)90057-P

Peptidases and proteases of Escherichia coli and Salmonella typhimurium

2 December 2022

84

1 reference

https://api.crossref.org/works/10.1016/1046-5928(91)90057-P

Characterization of degP, a gene required for proteolysis in the cell envelope and essential for growth of Escherichia coli at high temperature

2 December 2022

85

1 reference

https://api.crossref.org/works/10.1016/1046-5928(91)90057-P

Association of degradation and secretion of three chimeric polypeptides in Escherichia coli

2 December 2022

86

1 reference

https://api.crossref.org/works/10.1016/1046-5928(91)90057-P

Ubiquitin fusion augments the yield of cloned gene products in Escherichia coli

2 December 2022

87

1 reference

https://api.crossref.org/works/10.1016/1046-5928(91)90057-P

High-level expression of human insulin-like growth factor II in Escherichia coli

2 December 2022

90

1 reference

https://api.crossref.org/works/10.1016/1046-5928(91)90057-P

The kit ligand: A cell surface molecule altered in steel mutant fibroblasts

2 December 2022

94

1 reference

https://api.crossref.org/works/10.1016/1046-5928(91)90057-P

Novel method for identifying sequence-specific DNA-binding proteins.

2 December 2022

97

1 reference

https://api.crossref.org/works/10.1016/1046-5928(91)90057-P

Generation of polyclonal antibodies against purified rat whey acidic proteins and the synthesis of a tracer fusion protein suitable for use in radioimmunoassays

2 December 2022

99

1 reference

https://api.crossref.org/works/10.1016/1046-5928(91)90057-P

Affinity precipitation of enzymes

2 December 2022

101

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https://api.crossref.org/works/10.1016/1046-5928(91)90057-P

The affinity precipitation technique. Application to the isolation and purification of trypsin from bovine pancreas

2 December 2022

102

1 reference

https://api.crossref.org/works/10.1016/1046-5928(91)90057-P

Affinity Partitioning of Phosphofructokinase from Baker's Yeast Using Polymer-Bound Cibacron Blue F3G-A

2 December 2022

103

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https://api.crossref.org/works/10.1016/1046-5928(91)90057-P

2 December 2022

Identifiers

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18 August 2017

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