100% protein sequence coverage: a modern form of surrealism in proteomics (Q37772117)

22 August 2017

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title

100% protein sequence coverage: a modern form of surrealism in proteomics (English)

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22 August 2017

Bjoern Meyer

1

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22 August 2017

Dimitrios G Papasotiriou

series ordinal

2

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22 August 2017

Michael Karas

series ordinal

3

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22 August 2017

publication date

13 July 2010

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22 August 2017

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22 August 2017

volume

41

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22 August 2017

issue

2

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22 August 2017

page(s)

291-310

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Mass spectrometric analysis of integral membrane proteins at the subpicomolar level: application to rhodopsin

22 August 2017

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21 January 2018

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Straightforward and de novo peptide sequencing by MALDI-MS/MS using a Lys-N metalloendopeptidase.

21 January 2018

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Mass measurement and top-down HPLC/MS analysis of intact monoclonal antibodies on a hybrid linear quadrupole ion trap-Orbitrap mass spectrometer

21 January 2018

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A novel polyacrylamide gel system for proteomic use offering controllable pore expansion by crosslinker cleavage

21 January 2018

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The art of observing rare protein species in proteomes with peptide ligand libraries

21 January 2018

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Tandem mass spectrometry with ultrahigh mass accuracy clarifies peptide identification by database retrieval

21 January 2018

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Top-down identification and characterization of biomolecules by mass spectrometry

21 January 2018

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Overview on modern approaches to speed up protein identification workflows relying on enzymatic cleavage and mass spectrometry-based techniques

21 January 2018

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Relating electrospray ionization response to nonpolar character of small peptides.

21 January 2018

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Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry

21 January 2018

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Silk cocoon of Bombyx mori: proteins and posttranslational modifications--heavy phosphorylation and evidence for lysine-mediated cross links

21 January 2018

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Multiple enzymatic digestion for enhanced sequence coverage of proteins in complex proteomic mixtures using capillary LC with ion trap MS/MS.

21 January 2018

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Is proteomics the new genomics?

21 January 2018

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Status of complete proteome analysis by mass spectrometry: SILAC labeled yeast as a model system

21 January 2018

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Comprehensive mass-spectrometry-based proteome quantification of haploid versus diploid yeast

21 January 2018

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Cross signaling, cell specificity, and physiology

21 January 2018

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Protein cleavage strategies for an improved analysis of the membrane proteome

21 January 2018

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Toward the complete membrane proteome: high coverage of integral membrane proteins through transmembrane peptide detection

21 January 2018

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Achieving augmented limits of detection for peptides with hydrophobic alkyl tags

21 January 2018

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The complete amino acid sequence of human skeletal-muscle fructose-bisphosphate aldolase

21 January 2018

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Identification of the mitochondrial ND3 subunit as a structural component involved in the active/deactive enzyme transition of respiratory complex I.

21 January 2018

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https://api.crossref.org/works/10.1007%2FS00726-010-0680-6

What does the future hold for Top Down mass spectrometry?

21 January 2018

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https://api.crossref.org/works/10.1007%2FS00726-010-0680-6

Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach

21 January 2018

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https://api.crossref.org/works/10.1007%2FS00726-010-0680-6

Experimental evaluation of protein identification by an LC/MALDI/on-target digestion approach

21 January 2018

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https://api.crossref.org/works/10.1007%2FS00726-010-0680-6

Perspectives in spicing up proteomics with splicing.

21 January 2018

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https://api.crossref.org/works/10.1007%2FS00726-010-0680-6

Proteolysis and mass spectrometric analysis of an integral membrane: aquaporin 0.

21 January 2018

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https://api.crossref.org/works/10.1007%2FS00726-010-0680-6

Extending top-down mass spectrometry to proteins with masses greater than 200 kilodaltons

21 January 2018

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https://api.crossref.org/works/10.1007%2FS00726-010-0680-6

On-probe digestion of bacterial proteins for MALDI-MS.

21 January 2018

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https://api.crossref.org/works/10.1007%2FS00726-010-0680-6

Online microwave D-cleavage LC-ESI-MS/MS of intact proteins: site-specific cleavages at aspartic acid residues and disulfide bonds

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00726-010-0680-6

Electron transfer dissociation of peptides generated by microwave D-cleavage digestion of proteins.

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00726-010-0680-6

Far-field optical nanoscopy

21 January 2018

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https://api.crossref.org/works/10.1007%2FS00726-010-0680-6

Effect of chemical modifications on peptide fragmentation behavior upon electron transfer induced dissociation.

21 January 2018

1 reference

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The necessity of functional proteomics: protein species and molecular function elucidation exemplified by in vivo alpha A crystallin N-terminal truncation.

21 January 2018

1 reference

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Comparison of HPLC/ESI-FTICR MS versus MALDI-TOF/TOF MS for glycopeptide analysis of a highly glycosylated HIV envelope glycoprotein

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00726-010-0680-6

Chemical cleavage-assisted tryptic digestion for membrane proteome analysis

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00726-010-0680-6

Membrane protein identification: N-terminal labeling of nontryptic membrane protein peptides facilitates database searching

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00726-010-0680-6

4-Chloro-alpha-cyanocinnamic acid is an advanced, rationally designed MALDI matrix

21 January 2018

1 reference

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Comparison between the matrices alpha-cyano-4-hydroxycinnamic acid and 4-chloro-alpha-cyanocinnamic acid for trypsin, chymotrypsin, and pepsin digestions by MALDI-TOF mass spectrometry

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00726-010-0680-6

Mass spectrometrical verification of stomatin-like protein 2 (SLP-2) primary structure

21 January 2018

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The speciation of the proteome

21 January 2018

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https://api.crossref.org/works/10.1007%2FS00726-010-0680-6

A 2-D liquid separations/mass mapping method for interlysate comparison of ovarian cancers

21 January 2018

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https://api.crossref.org/works/10.1007%2FS00726-010-0680-6

Sensitive and specific identification of wild type and variant proteins from 8 to 669 kDa using top-down mass spectrometry

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00726-010-0680-6

Fragmentation characteristics of collision-induced dissociation in MALDI TOF/TOF mass spectrometry

21 January 2018

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https://api.crossref.org/works/10.1007%2FS00726-010-0680-6

Phosphoric acid as a matrix additive for MALDI MS analysis of phosphopeptides and phosphoproteins.

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00726-010-0680-6

The dominance of arginine-containing peptides in MALDI-derived tryptic mass fingerprints of proteins

21 January 2018

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https://api.crossref.org/works/10.1007%2FS00726-010-0680-6

B. subtilis ribosomal proteins: structural homology and post-translational modifications

21 January 2018

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https://api.crossref.org/works/10.1007%2FS00726-010-0680-6

Centrifugal methods and devices for rapid in-gel digestion of proteins

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00726-010-0680-6

Chemical cleavage at aspartyl residues for protein identification

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00726-010-0680-6

Recent advances in immobilized enzymatic reactors and their applications in proteome analysis

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00726-010-0680-6

Shotgun identification of protein modifications from protein complexes and lens tissue

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00726-010-0680-6

Top-down protein sequencing and MS3 on a hybrid linear quadrupole ion trap-orbitrap mass spectrometer.

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00726-010-0680-6

Electrostatic axially harmonic orbital trapping: a high-performance technique of mass analysis

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00726-010-0680-6

Computational prediction of proteotypic peptides for quantitative proteomics.

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00726-010-0680-6

Precision proteomics: the case for high resolution and high mass accuracy

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00726-010-0680-6

Comparison of electrospray and matrix-assisted laser desorption ionization on the same hybrid quadrupole time-of-flight tandem mass spectrometer: application to bidimensional liquid chromatography of proteins from bovine milk fraction

21 January 2018

1 reference

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Dihydrofolate reductase: low-resolution mass-spectrometric analysis of an elastase digest as a sequencing tool

21 January 2018

1 reference

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Peptide mass fingerprinting after less specific in-gel proteolysis using MALDI-LTQ-Orbitrap and 4-chloro-alpha-cyanocinnamic acid

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00726-010-0680-6

Membrane proteins and proteomics: love is possible, but so difficult

21 January 2018

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https://api.crossref.org/works/10.1007%2FS00726-010-0680-6

Power and limitations of electrophoretic separations in proteomics strategies.

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00726-010-0680-6

Development of a microfluidics-based gel protein recovery system.

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00726-010-0680-6

Acid hydrolysis of proteins in matrix assisted laser desorption ionization matrices

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00726-010-0680-6

Elastase digests: new ammunition for shotgun membrane proteomics

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00726-010-0680-6

Membrane protein analysis using an improved peptic in-solution digestion protocol

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00726-010-0680-6

The benefit of combining nLC-MALDI-Orbitrap MS data with nLC-MALDI-TOF/TOF data for proteomic analyses employing elastase

21 January 2018

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Proteome analysis in the clinical chemistry laboratory: myth or reality?

21 January 2018

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Prefractionation techniques in proteome analysis: the mining tools of the third millennium

21 January 2018

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Protein purification by Off-Gel electrophoresis.

21 January 2018

1 reference

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Multiplexed protein quantitation in Saccharomyces cerevisiae using amine-reactive isobaric tagging reagents

21 January 2018

1 reference

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Finding one's way in proteomics: a protein species nomenclature

21 January 2018

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In-gel digestion for mass spectrometric characterization of proteins and proteomes

21 January 2018

1 reference

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Novel acrylamido monomers with higher hydrophilicity and improved hydrolytic stability: I. Synthetic route and product characterization.

21 January 2018

1 reference

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Using size exclusion chromatography-RPLC and RPLC-CIEF as two-dimensional separation strategies for protein profiling

21 January 2018

1 reference

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Is there a code embedded in proteins that is based on post-translational modifications?

21 January 2018

1 reference

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Proteomics of integral membrane proteins--theory and application

21 January 2018

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Immobilized monolithic enzyme reactors for application in proteomics and pharmaceutics

21 January 2018

1 reference

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A capillary monolithic trypsin reactor for efficient protein digestion in online and offline coupling to ESI and MALDI mass spectrometry

21 January 2018

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18O labeling: a tool for proteomics

21 January 2018

1 reference

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Characterization of the 70S Ribosome from Rhodopseudomonas palustris using an integrated "top-down" and "bottom-up" mass spectrometric approach

21 January 2018

1 reference

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Supplemental activation method for high-efficiency electron-transfer dissociation of doubly protonated peptide precursors

21 January 2018

1 reference

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Decision tree-driven tandem mass spectrometry for shotgun proteomics

21 January 2018

1 reference

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Value of using multiple proteases for large-scale mass spectrometry-based proteomics

21 January 2018

1 reference

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Rapid chemical digestion of small acid-soluble spore proteins for analysis of Bacillus spores

21 January 2018

1 reference

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Integration of residue-specific acid cleavage into proteomic workflows

21 January 2018

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https://api.crossref.org/works/10.1007%2FS00726-010-0680-6

Analysis of a model virus using residue-specific chemical cleavage and MALDI-TOF mass spectrometry

21 January 2018

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Evaluation of microwave-accelerated residue-specific acid cleavage for proteomic applications

21 January 2018

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Peptide and protein sequence analysis by electron transfer dissociation mass spectrometry

21 January 2018

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Top-down mass spectrometry of a 29-kDa protein for characterization of any posttranslational modification to within one residue

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00726-010-0680-6

Straightforward ladder sequencing of peptides using a Lys-N metalloendopeptidase.

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00726-010-0680-6

Gel-eluted liquid fraction entrapment electrophoresis: an electrophoretic method for broad molecular weight range proteome separation

21 January 2018

1 reference

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Laserspray ionization, a new atmospheric pressure MALDI method for producing highly charged gas-phase ions of peptides and proteins directly from solid solutions

21 January 2018

1 reference

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Combined in-gel tryptic digestion and CNBr cleavage for the generation of peptide maps of an integral membrane protein with MALDI-TOF mass spectrometry

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00726-010-0680-6

Improved in-gel approaches to generate peptide maps of integral membrane proteins with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00726-010-0680-6

Size-sorting combined with improved nanocapillary liquid chromatography-mass spectrometry for identification of intact proteins up to 80 kDa

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00726-010-0680-6

Integrating 'top-down" and "bottom-up" mass spectrometric approaches for proteomic analysis of Shewanella oneidensis

21 January 2018

1 reference

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Isoelectric focusing nonporous RP HPLC: a two-dimensional liquid-phase separation method for mapping of cellular proteins with identification using MALDI-TOF mass spectrometry

21 January 2018

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https://api.crossref.org/works/10.1007%2FS00726-010-0680-6

Large-scale analysis of the yeast proteome by multidimensional protein identification technology

21 January 2018

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Progress with gene-product mapping of the Mollicutes: Mycoplasma genitalium.

21 January 2018

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Top-down mass spectrometry of integral membrane proteins

21 January 2018

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https://api.crossref.org/works/10.1007%2FS00726-010-0680-6

Progress with proteome projects: why all proteins expressed by a genome should be identified and how to do it.

21 January 2018

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STED microscopy reveals that synaptotagmin remains clustered after synaptic vesicle exocytosis

21 January 2018

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Universal sample preparation method for proteome analysis

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00726-010-0680-6

A strategy to locate cysteine residues in proteins by specific chemical cleavage followed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

21 January 2018

1 reference

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A method for the comprehensive proteomic analysis of membrane proteins

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00726-010-0680-6

Extended Range Proteomic Analysis (ERPA): a new and sensitive LC-MS platform for high sequence coverage of complex proteins with extensive post-translational modifications-comprehensive analysis of beta-casein and epidermal growth factor receptor (E

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00726-010-0680-6

On-line LC-MS approach combining collision-induced dissociation (CID), electron-transfer dissociation (ETD), and CID of an isolated charge-reduced species for the trace-level characterization of proteins with post-translational modifications

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00726-010-0680-6

An integrated top-down and bottom-up strategy for broadly characterizing protein isoforms and modifications

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00726-010-0680-6

Integrated workflow for characterizing intact phosphoproteins from complex mixtures

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00726-010-0680-6

Complete amino acid sequences of chitinase-1 and -2 from bulbs of genus Tulipa

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00726-010-0680-6

A comparison of drug-treated and untreated HCT-116 human colon adenocarcinoma cells using a 2-D liquid separation mapping method based upon chromatofocusing PI fractionation

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00726-010-0680-6

Automated integration of monolith-based protein separation with on-plate digestion for mass spectrometric analysis of esophageal adenocarcinoma human epithelial samples

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00726-010-0680-6

Increased coverage in the transmembrane domain with activated-ion electron capture dissociation for top-down Fourier-transform mass spectrometry of integral membrane proteins

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00726-010-0680-6

ProSight PTM 2.0: improved protein identification and characterization for top down mass spectrometry

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00726-010-0680-6

Ultratrace liquid chromatography/mass spectrometry analysis of large peptides with post-translational modifications using narrow-bore poly(styrene-divinylbenzene) monolithic columns and extended range proteomic analysis.

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00726-010-0680-6

Liquid chromatography electrospray ionization and matrix-assisted laser desorption ionization tandem mass spectrometry for the analysis of lipid raft proteome of monocytes

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00726-010-0680-6

Monolithic column HPLC separation of intact proteins analyzed by LC-MALDI using on-plate digestion: An approach to integrate protein separation and identification

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00726-010-0680-6

Microwave-assisted acid hydrolysis of proteins combined with liquid chromatography MALDI MS/MS for protein identification

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00726-010-0680-6

High Sequence Coverage of Proteins Isolated from Liquid Separations of Breast Cancer Cells Using Capillary Electrophoresis-Time-of-Flight MS and MALDI-TOF MS Mapping

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00726-010-0680-6

Electron Capture Dissociation of Multiply Charged Protein Cations. A Nonergodic Process

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00726-010-0680-6

Proteomic Profiling of Intact Proteins Using WAX-RPLC 2-D Separations and FTICR Mass Spectrometry

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00726-010-0680-6

Evaluation of the Low-Specificity Protease Elastase for Large-Scale Phosphoproteome Analysis

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00726-010-0680-6

10.1007/S00726-010-0680-6

21 January 2018

Identifiers

DOI

1 reference

22 August 2017

1 reference