Proteins on the move: insights gained from fluorescent protein technologies (Q37937419)

25 August 2017

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title

Proteins on the move: insights gained from fluorescent protein technologies (English)

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25 August 2017

Atsushi Miyawaki

1

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25 August 2017

publication date

23 September 2011

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Nature Reviews Molecular Cell Biology

25 August 2017

published in

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25 August 2017

volume

12

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25 August 2017

issue

10

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25 August 2017

page(s)

656-668

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The green fluorescent protein

25 August 2017

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Creating new fluorescent probes for cell biology.

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Innovation: Photoactivatable fluorescent proteins.

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Visualization of molecular interactions by fluorescence complementation

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High-throughput fluorescence microscopy for systems biology

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Fluorescent probes for super-resolution imaging in living cells

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Imaging gene expression in single living cells.

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A monomeric red fluorescent protein

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The structural basis for red fluorescence in the tetrameric GFP homolog DsRed

31 March 2020

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Refined crystal structure of DsRed, a red fluorescent protein from coral, at 2.0-A resolution

31 March 2020

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Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein

31 March 2020

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EosFP, a fluorescent marker protein with UV-inducible green-to-red fluorescence conversion

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A strategy for the generation of non-aggregating mutants of Anthozoa fluorescent proteins

31 March 2020

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GFP-like proteins stably accumulate in lysosomes

31 March 2020

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Semi-rational engineering of a coral fluorescent protein into an efficient highlighter

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Diffusion of large molecules into assembling nuclei revealed using an optical highlighting technique

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Expression of fluorescently tagged connexins: a novel approach to rescue function of oligomeric DsRed-tagged proteins

31 March 2020

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Hetero-oligomeric tagging diminishes non-specific aggregation of target proteins fused with Anthozoa fluorescent proteins.

31 March 2020

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Mobility measurement by analysis of fluorescence photobleaching recovery kinetics

31 March 2020

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Cellular determinants of the lateral mobility of neural cell adhesion molecules

31 March 2020

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Nuclear membrane dynamics and reassembly in living cells: targeting of an inner nuclear membrane protein in interphase and mitosis

31 March 2020

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FRAP analysis of binding: proper and fitting

31 March 2020

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Diffusional mobility of Golgi proteins in membranes of living cells

31 March 2020

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Exchange of protein molecules through connections between higher plant plastids

31 March 2020

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Golgi membranes are absorbed into and reemerge from the ER during mitosis

31 March 2020

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Reduced mobility of the alternate splicing factor (ASF) through the nucleoplasm and steady state speckle compartments

31 March 2020

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Photobleaching recovery and anisotropy decay of green fluorescent protein GFP-S65T in solution and cells: cytoplasmic viscosity probed by green fluorescent protein translational and rotational diffusion

31 March 2020

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Rapid diffusion of green fluorescent protein in the mitochondrial matrix

31 March 2020

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Diffusion of green fluorescent protein in the aqueous-phase lumen of endoplasmic reticulum

31 March 2020

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Photoactivatable fluorescent proteins for diffraction-limited and super-resolution imaging

31 March 2020

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The structure and function of fluorescent proteins

31 March 2020

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Conversion of the monomeric red fluorescent protein into a photoactivatable probe

31 March 2020

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Photoactivatable mCherry for high-resolution two-color fluorescence microscopy

31 March 2020

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Direct measurement of protein dynamics inside cells using a rationally designed photoconvertible protein

31 March 2020

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A bright and photostable photoconvertible fluorescent protein

31 March 2020

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mKikGR, a monomeric photoswitchable fluorescent protein

31 March 2020

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Engineering of a monomeric green-to-red photoactivatable fluorescent protein induced by blue light

31 March 2020

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A photoactivatable marker protein for pulse-chase imaging with superresolution.

31 March 2020

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Photoswitchable cyan fluorescent protein for protein tracking

31 March 2020

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A photoswitchable orange-to-far-red fluorescent protein, PSmOrange

31 March 2020

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Cell-cycle-dependent binding kinetics for the early endosomal tethering factor EEA1.

31 March 2020

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Oct4 kinetics predict cell lineage patterning in the early mammalian embryo

31 March 2020

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Direct measurement of coupling between dendritic spines and shafts.

31 March 2020

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Deep tissue two-photon microscopy

31 March 2020

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Measurement of molecular diffusion in solution by multiphoton fluorescence photobleaching recovery

31 March 2020

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Rapid turnover of actin in dendritic spines and its regulation by activity.

31 March 2020

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The subspine organization of actin fibers regulates the structure and plasticity of dendritic spines

31 March 2020

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Rapid redistribution of synaptic PSD-95 in the neocortex in vivo

31 March 2020

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Distinct domains within PSD-95 mediate synaptic incorporation, stabilization, and activity-dependent trafficking

31 March 2020

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Natural animal coloration can Be determined by a nonfluorescent green fluorescent protein homolog

31 March 2020

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Kindling fluorescent proteins for precise in vivo photolabeling

31 March 2020

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Regulated fast nucleocytoplasmic shuttling observed by reversible protein highlighting

31 March 2020

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Highlighted generation of fluorescence signals using simultaneous two-color irradiation on Dronpa mutants

31 March 2020

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Molecular Basis of the Light-driven Switching of the Photochromic Fluorescent Protein Padron

31 March 2020

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Structural basis for reversible photobleaching of a green fluorescent protein homologue

31 March 2020

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Structural characterization of IrisFP, an optical highlighter undergoing multiple photo-induced transformations

31 March 2020

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Generation of monomeric reversibly switchable red fluorescent proteins for far-field fluorescence nanoscopy

31 March 2020

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Red fluorescent protein with reversibly photoswitchable absorbance for photochromic FRET.

31 March 2020

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Lateral propagation of EGF signaling after local stimulation is dependent on receptor density.

31 March 2020

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Visualization of the spatial and temporal dynamics of intracellular signaling

31 March 2020

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Nanometre-level analysis demonstrates that lipid flow does not drive membrane glycoprotein movements

31 March 2020

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Imaging of single molecule diffusion

31 March 2020

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Hierarchical organization of the plasma membrane: investigations by single-molecule tracking vs. fluorescence correlation spectroscopy

31 March 2020

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Single molecule imaging of fluorophores and enzymatic reactions achieved by objective-type total internal reflection fluorescence microscopy

31 March 2020

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Visualizing single molecules inside living cells using total internal reflection fluorescence microscopy.

31 March 2020

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Total internal reflection fluorescence microscopy: technical innovations and novel applications

31 March 2020

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Chapter 7: Total internal reflection fluorescence microscopy

31 March 2020

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A new wave of cellular imaging.

31 March 2020

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Fusion of constitutive membrane traffic with the cell surface observed by evanescent wave microscopy

31 March 2020

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Imaging actin and dynamin recruitment during invagination of single clathrin-coated pits

31 March 2020

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Highly inclined thin illumination enables clear single-molecule imaging in cells.

31 March 2020

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Improving the photostability of bright monomeric orange and red fluorescent proteins

31 March 2020

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Bright monomeric red fluorescent protein with an extended fluorescence lifetime

31 March 2020

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Chapter 1: In vivo applications of fluorescence correlation spectroscopy

31 March 2020

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Fluorescence correlation spectroscopy in living cells

31 March 2020

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Fluorescence correlation spectroscopy: novel variations of an established technique

31 March 2020

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Signal analysis of total internal reflection fluorescent speckle microscopy (TIR-FSM) and wide-field epi-fluorescence FSM of the actin cytoskeleton and focal adhesions in living cells.

31 March 2020

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Quantitative fluorescent speckle microscopy of cytoskeleton dynamics

31 March 2020

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Imaging intracellular fluorescent proteins at nanometer resolution

31 March 2020

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Ultra-high resolution imaging by fluorescence photoactivation localization microscopy

31 March 2020

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Sub-diffraction-limit imaging by stochastic optical reconstruction microscopy (STORM)

31 March 2020

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High-density mapping of single-molecule trajectories with photoactivated localization microscopy

31 March 2020

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In vivo imaging of single-molecule translocation through nuclear pore complexes by pair correlation functions

31 March 2020

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Quantitation of membrane receptor distributions by image correlation spectroscopy: concept and application

31 March 2020

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Raster image correlation spectroscopy (RICS) for measuring fast protein dynamics and concentrations with a commercial laser scanning confocal microscope

31 March 2020

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Quantitative fluorescence imaging of protein diffusion and interaction in living cells

31 March 2020

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Determining protease activity in vivo by fluorescence cross-correlation analysis

31 March 2020

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A fluorescent variant of a protein from the stony coral Montipora facilitates dual-color single-laser fluorescence cross-correlation spectroscopy

31 March 2020

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Monomeric red fluorescent proteins with a large Stokes shift

31 March 2020

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Monitoring protein conformations and interactions by fluorescence resonance energy transfer between mutants of green fluorescent protein

31 March 2020

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31 March 2020

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Imaging molecular interactions in living cells by FRET microscopy

31 March 2020

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Fluorescent protein FRET: the good, the bad and the ugly

31 March 2020

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Genetically encoded biosensors based on engineered fluorescent proteins.

31 March 2020

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Development of probes for cellular functions using fluorescent proteins and fluorescence resonance energy transfer

31 March 2020

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Quantitative fluorescence resonance energy transfer measurements using fluorescence microscopy

31 March 2020

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FRET two-hybrid mapping reveals function and location of L-type Ca2+ channel CaM preassociation.

31 March 2020

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FRET or no FRET: a quantitative comparison

31 March 2020

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Supersensitive Ras activation in dendrites and spines revealed by two-photon fluorescence lifetime imaging

31 March 2020

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Local, persistent activation of Rho GTPases during plasticity of single dendritic spines

31 March 2020

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The mechanisms underlying the spatial spreading of signaling activity

31 March 2020

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Visualization of molecular interactions using bimolecular fluorescence complementation analysis: characteristics of protein fragment complementation

31 March 2020

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Dual-color superresolution imaging of genetically expressed probes within individual adhesion complexes

31 March 2020

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Signaling from the living plasma membrane

31 March 2020

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Generation of transgenic non-human primates with germline transmission

31 March 2020

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In vivo imaging of extracellular matrix remodeling by tumor-associated fibroblasts

31 March 2020

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Visualisation and quantification of morphogen gradient formation in the zebrafish

31 March 2020

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Modular scanning FCS quantifies receptor-ligand interactions in living multicellular organisms.

31 March 2020

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A role for actin arcs in the leading-edge advance of migrating cells

31 March 2020

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Phototransformation of green fluorescent protein with UV and visible light leads to decarboxylation of glutamate 222

31 March 2020

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Photo-induced peptide cleavage in the green-to-red conversion of a fluorescent protein.

31 March 2020

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Analysis of fluorophore diffusion by continuous distributions of diffusion coefficients: application to photobleaching measurements of multicomponent and anomalous diffusion

31 March 2020

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Anomalous diffusion reports on the interaction of misfolded proteins with the quality control machinery in the endoplasmic reticulum

31 March 2020

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Molecular crowding affects diffusion and binding of nuclear proteins in heterochromatin and reveals the fractal organization of chromatin

31 March 2020

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Chemical labeling strategies for cell biology

31 March 2020

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Single-particle tracking photoactivated localization microscopy for mapping single-molecule dynamics

31 March 2020

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Fluorescence correlation spectroscopy with autofluorescent proteins.

31 March 2020

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PubMed

https://pubmed.ncbi.nlm.nih.gov/21941275

inferred from PubMed ID database lookup

12 December 2020

based on heuristic

Identifiers

DOI

10.1038/NRM3199

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25 August 2017

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