Tissue fixation and the effect of molecular fixatives on downstream staining procedures (Q38190394)

30 August 2017

1 reference

19 September 2019

Tissue fixation and the effect of molecular fixatives on downstream staining procedures (English)

1 reference

biomedical investigative technique

30 August 2017

1 reference

19 September 2019

William J Howat

1

1 reference

PMCID

4240801

30 August 2017

Beverley A Wilson

series ordinal

2

1 reference

30 August 2017

21 February 2014

1 reference

PMCID

4240801

30 August 2017

1 November 2014

1 reference

19 September 2019

2 references

30 August 2017

19 September 2019

70

1 reference

30 August 2017

1

1 reference

30 August 2017

12-19

1 reference

Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported

30 August 2017

1 reference

Guidelines and considerations for conducting experiments using tissue microarrays

19 September 2019

0 references

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4240801

Magnetization transfer MRI in pancreatic cancer xenograft models.

6 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4240801

The HOPE fixation technique--a promising alternative to common prostate cancer biobanking approaches

6 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4240801

Histological assessment of PAXgene tissue fixation and stabilization reagents

6 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4240801

Formalin fixation at low temperature better preserves nucleic acid integrity.

6 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4240801

Protein and gene expression of estrogen receptor alpha and nuclear morphology of two breast cancer cell lines after different fixation methods.

6 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4240801

The impact of tissue fixatives on morphology and antibody-based protein profiling in tissues and cells.

6 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4240801

Choice of fixative is crucial to successful immunohistochemical detection of phosphoproteins in paraffin-embedded tumor tissues

6 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4240801

Preservation of biomolecules in breast cancer tissue by a formalin-free histology system

6 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4240801

Novel zinc-based fixative for high quality DNA, RNA and protein analysis

6 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4240801

Optimization of recovery of RNA from formalin-fixed, paraffin-embedded tissue

6 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4240801

RCL2, a new fixative, preserves morphology and nucleic acid integrity in paraffin-embedded breast carcinoma and microdissected breast tumor cells.

6 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4240801

Experience with an automated microwave-assisted rapid tissue processing method: validation of histologic quality and impact on the timeliness of diagnostic surgical pathology

6 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4240801

A molecular mechanism of formalin fixation and antigen retrieval

6 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4240801

Modeling formalin fixation and antigen retrieval with bovine pancreatic RNase A II. Interrelationship of cross-linking, immunoreactivity, and heat treatment

6 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4240801

Measurement of gene expression in archival paraffin-embedded tissues: development and performance of a 92-gene reverse transcriptase-polymerase chain reaction assay

6 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4240801

A Tissue Fixative that Protects Macromolecules (DNA, RNA, and Protein) and Histomorphology in Clinical Samples

6 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4240801

Zinc-based fixative improves preservation of genomic DNA and proteins in histoprocessing of human tissues

6 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4240801

Effect of fixatives and tissue processing on the content and integrity of nucleic acids

6 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4240801

Evaluation of non-formalin tissue fixation for molecular profiling studies

6 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4240801

Methacarn fixation: a novel tool for analysis of gene expressions in paraffin-embedded tissue specimens

6 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4240801

A high frequency of sequence alterations is due to formalin fixation of archival specimens

6 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4240801

Tissue microarrays for high-throughput molecular profiling of tumor specimens

6 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4240801

Antigen retrieval in formalin-fixed, paraffin-embedded tissues: an enhancement method for immunohistochemical staining based on microwave oven heating of tissue sections

6 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4240801

Systematic comparison of tissue fixation with alternative fixatives to conventional tissue fixation with buffered formalin in a xenograft-based model

6 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4240801

6 September 2017

Formaldehyde fixation

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4240801

Formaldehyde as a probe of DNA structure. II. Reaction with endocyclic imino groups of DNA bases

14 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4240801

Formaldehyde as a probe of DNA structure. I. Reaction with exocyclic amino groups of DNA bases

14 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4240801

What's cooking? detection of important biomarkers in HOPE-fixed, paraffin-embedded tissues eliminates the need for antigen retrieval.

14 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4240801

Quantitative comparison of immunohistochemical staining intensity in tissues fixed in formalin and Histochoice.

23 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4240801

Preservation of nucleic acids and tissue morphology in paraffin-embedded clinical samples: comparison of five molecular fixatives

23 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4240801

Will PAXgene substitute formalin? A morphological and molecular comparative study using a new fixative system.

31 August 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4240801

Nucleic acid quantity and quality from paraffin blocks: defining optimal fixation, processing and DNA/RNA extraction techniques.

31 August 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4240801

Comparison of formalin-free tissue fixatives: a proteomic study testing their application for routine pathology and research.

31 August 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4240801

Alcohol based tissue fixation as an alternative for formaldehyde: influence on immunohistochemistry

31 August 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4240801

Fixation of brain tumor biopsy specimens with RCL2 results in well-preserved histomorphology, immunohistochemistry and nucleic acids.

31 August 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4240801

The reaction of formaldehyde with proteins; cross-linking between amino and primary amide or guanidyl groups.

31 August 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4240801

Comparison of formalin and FineFIX in preserving DNA material in small biopsies

31 August 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4240801

Ultrasound-accelerated tissue fixation/processing achieves superior morphology and macromolecule integrity with storage stability.

31 August 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4240801

HOPE fixation: a novel fixing method and paraffin-embedding technique for human soft tissues.

31 August 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4240801

The duration of fixation influences the yield of HCV cDNA-PCR products from formalin-fixed, paraffin-embedded liver tissue

31 August 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4240801

Fixation in cytochemistry and electron-microscopy

31 August 2018

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2014.01.022

Carnoy fixation: practical and theoretical considerations

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2014.01.022

Effects of formaldehyde fixation on protein secondary structure: a calorimetric and infrared spectroscopic investigation

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2014.01.022

Rates of penetration of fixing fluids

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2014.01.022

Formaldehyde Substitute Fixatives

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2014.01.022

Immunohistochemistry of tissue prepared by a molecular-friendly fixation and processing system

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2014.01.022

Improved immunohistochemical localization of tissue antigens using modified methacarn fixation

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2014.01.022

How formalin affects the outcome of routine and special stains

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2014.01.022

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2014.01.022

A method of rapid fixation of large biopsy specimens using microwave irradiation

7 January 2021

1 reference

12 December 2020

Methacarn (methanol-Carnoy) fixation. Practical and theoretical considerations.

1 reference

12 December 2020

Enzyme histochemistry and immunohistochemistry on biopsy specimens of pathologic human bone marrow

1 reference

12 December 2020

Establishment of the formalin-free surgical pathology laboratory. Utility of an alcohol-based fixative

1 reference

12 December 2020

10.1016/J.YMETH.2014.01.022

Identifiers

DOI

1 reference

30 August 2017

1 reference

30 August 2017

1 reference

PMCID

4240801