Improved seamless mutagenesis by recombineering using ccdB for counterselection (Q38257003)

1 September 2017

Improved seamless mutagenesis by recombineering using ccdB for counterselection (English)

1 reference

1 September 2017

5

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:24369425%20AND%20SRC:MED&resulttype=core&format=json

12 June 2020

Xiaoying Bian

2

1 reference

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12 June 2020

author name string

Hailong Wang

1

1 reference

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12 June 2020

Liqiu Xia

3

1 reference

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12 June 2020

Xuezhi Ding

4

1 reference

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12 June 2020

Youming Zhang

6

1 reference

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12 June 2020

Jun Fu

7

1 reference

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12 June 2020

A Francis Stewart

8

1 reference

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12 June 2020

language of work or name

English

0 references

publication date

24 December 2013

1 reference

1 September 2017

Nucleic Acids Research

1 reference

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12 June 2020

volume

42

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12 June 2020

issue

5

1 reference

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12 June 2020

page(s)

e37

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Creative Commons Attribution-NonCommercial 3.0 Unported

12 June 2020

copyright license

start time

4 October 2016

1 reference

April 2022 Public Data File from Crossref

copyright status

copyrighted

0 references

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6 September 2017

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Rejuvenation of CcdB-poisoned gyrase by an intrinsically disordered protein domain

6 September 2017

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Crystal Structure of E. coli RecE Protein Reveals a Toroidal Tetramer for Processing Double-Stranded DNA Breaks

6 September 2017

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Recombineering: a homologous recombination-based method of genetic engineering

6 September 2017

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A new positive/negative selection scheme for precise BAC recombineering

6 September 2017

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BAC TransgeneOmics: a high-throughput method for exploration of protein function in mammals

6 September 2017

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Recombineering: in vivo genetic engineering in E. coli, S. enterica, and beyond

6 September 2017

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Construction of a Vibrio splendidus mutant lacking the metalloprotease gene vsm by use of a novel counterselectable suicide vector

6 September 2017

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Efficient and seamless DNA recombineering using a thymidylate synthase A selection system in Escherichia coli

6 September 2017

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Simple and highly efficient BAC recombineering using galK selection

6 September 2017

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Toxins-antitoxins: plasmid maintenance, programmed cell death, and cell cycle arrest

6 September 2017

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High-throughput engineering of the mouse genome coupled with high-resolution expression analysis

6 September 2017

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Phage annealing proteins promote oligonucleotide-directed mutagenesis in Escherichia coli and mouse ES cells

6 September 2017

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Genetic engineering using homologous recombination

6 September 2017

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Molecular interactions of the CcdB poison with its bacterial target, the DNA gyrase

6 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3950717

Recombineering: a powerful new tool for mouse functional genomics

6 September 2017

1 reference

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The ratio between CcdA and CcdB modulates the transcriptional repression of the ccd poison-antidote system

6 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3950717

High efficiency mutagenesis, repair, and engineering of chromosomal DNA using single-stranded oligonucleotides

6 September 2017

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Techniques: Recombinogenic engineering--new options for cloning and manipulating DNA.

6 September 2017

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Point mutation of bacterial artificial chromosomes by ET recombination

6 September 2017

1 reference

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RecE/RecT and Redalpha/Redbeta initiate double-stranded break repair by specifically interacting with their respective partners

6 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3950717

One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products

6 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3950717

An efficient recombination system for chromosome engineering in Escherichia coli

6 September 2017

1 reference

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Toxin-antitoxin modules may regulate synthesis of macromolecules during nutritional stress

6 September 2017

1 reference

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Addiction modules and programmed cell death and antideath in bacterial cultures

6 September 2017

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Rings and filaments of beta protein from bacteriophage lambda suggest a superfamily of recombination proteins

6 September 2017

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A new logic for DNA engineering using recombination in Escherichia coli

6 September 2017

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Toroidal structure of lambda-exonuclease

6 September 2017

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ATP-dependent degradation of CcdA by Lon protease. Effects of secondary structure and heterologous subunit interactions

6 September 2017

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Positive-selection vectors using the F plasmid ccdB killer gene

6 September 2017

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Electron microscopic visualization of RecT protein and its complexes with DNA.

6 September 2017

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Mini-F plasmid genes that couple host cell division to plasmid proliferation

6 September 2017

1 reference

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Effects of the ccd function of the F plasmid on bacterial growth

6 September 2017

1 reference

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An active serine is involved in covalent substrate amino acid binding at each reaction center of gramicidin S synthetase.

6 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3950717

Cell killing by the F plasmid CcdB protein involves poisoning of DNA-topoisomerase II complexes

6 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3950717

High-efficiency counterselection recombineering for site-directed mutagenesis in bacterial artificial chromosomes

6 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3950717

Modification of human beta-globin locus PAC clones by homologous recombination in Escherichia coli

6 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3950717

Counter-selection recombineering of the baculovirus genome: a strategy for seamless modification of repeat-containing BACs

6 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3950717

Rapid modification of bacterial artificial chromosomes by ET-recombination.

14 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3950717

Antibiotic-free selection in E. coli: new considerations for optimal design and improved production

14 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3950717

Construction of a novel zero background prokaryotic expression vector: potential advantages

14 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3950717

Mutations of temperature sensitivity in R plasmid pSC101

14 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3950717

Lon-dependent proteolysis of CcdA is the key control for activation of CcdB in plasmid-free segregant bacteria.

14 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3950717

Full-length RecE enhances linear-linear homologous recombination and facilitates direct cloning for bioprospecting.

23 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3950717

A versatile zero background T-vector system for gene cloning and functional genomics

31 August 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3950717

Recombineering with tolC as a selectable/counter-selectable marker: remodeling the rRNA operons of Escherichia coli

31 August 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3950717

A recombineering pipeline for functional genomics applied to Caenorhabditis elegans.

31 August 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3950717

Two-step red-mediated recombination for versatile high-efficiency markerless DNA manipulation in Escherichia coli

31 August 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3950717

An improved recombineering approach by adding RecA to lambda Red recombination

31 August 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3950717

Separate-component-stabilization system for protein and DNA production without the use of antibiotics

31 August 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3950717

Molecular basis of gyrase poisoning by the addiction toxin CcdB.

31 August 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3950717

The genome sequence of the entomopathogenic bacterium Photorhabdus luminescens

31 August 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3950717

Targeted modification of a human beta-globin locus BAC clone using GET Recombination and an I-Scei counterselection cassette.

31 August 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3950717

Engineering the mouse genome with bacterial artificial chromosomes to create multipurpose alleles.

31 August 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3950717

Site-specific gene targeting in mouse embryonic stem cells with intact bacterial artificial chromosomes.

31 August 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3950717

Conformational Adaptability of Redβ during DNA Annealing and Implications for Its Structural Relationship with Rad52

31 August 2018

1 reference

Crossref

https://api.crossref.org/works/10.1093%2FNAR%2FGKT1339

DNA cloning by homologous recombination in Escherichia coli

21 January 2018

1 reference

12 December 2020

Cloning vectors for expression-PCR products

1 reference

12 December 2020

Bacterial death by DNA gyrase poisoning

1 reference

12 December 2020

A recombineering pipeline to make conditional targeting constructs

1 reference

12 December 2020

Identifiers

DOI

10.1093/NAR/GKT1339

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:24369425%20AND%20SRC:MED&resulttype=core&format=json

12 June 2020

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:24369425%20AND%20SRC:MED&resulttype=core&format=json

12 June 2020

PubMed publication ID

24369425

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:24369425%20AND%20SRC:MED&resulttype=core&format=json