Transient recombinant protein expression in a human amniocyte cell line: the CAP-T® cell system (Q39367320)

11 September 2017

title

Transient recombinant protein expression in a human amniocyte cell line: the CAP-T® cell system (English)

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11 September 2017

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Simon Fischer

1

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Nadine Charara

2

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Andrea Gerber

3

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Jens Wölfel

4

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Gudrun Schiedner

5

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Bernd Voedisch

6

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Sabine Geisse

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publication date

17 April 2012

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Biotechnology and Bioengineering

11 September 2017

published in

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volume

109

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issue

9

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page(s)

2250-2261

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Amniocytes can serve a dual function as a source of iPS cells and feeder layers

11 September 2017

cites work

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High-density transfection with HEK-293 cells allows doubling of transient titers and removes need for a priori DNA complex formation with PEI.

21 January 2018

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Valproic acid: a viable alternative to sodium butyrate for enhancing protein expression in mammalian cell cultures

21 January 2018

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Recombinant protein production by large-scale transient gene expression in mammalian cells: state of the art and future perspectives

21 January 2018

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A versatile vector for gene and oligonucleotide transfer into cells in culture and in vivo: polyethylenimine

21 January 2018

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Overcoming the nuclear barrier: cell cycle independent nonviral gene transfer with linear polyethylenimine or electroporation

21 January 2018

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Limiting factors governing protein expression following polyethylenimine-mediated gene transfer in HEK293-EBNA1 cells

21 January 2018

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Silencing of episomal transgene expression by plasmid bacterial DNA elements in vivo

21 January 2018

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Versatile expression system for rapid and stable production of recombinant proteins

21 January 2018

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Enhanced CHO cell-based transient gene expression with the epi-CHO expression system

21 January 2018

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Secreted placental alkaline phosphatase as a eukaryotic reporter gene

21 January 2018

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Expression systems for therapeutic glycoprotein production.

21 January 2018

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High-level and high-throughput recombinant protein production by transient transfection of suspension-growing human 293-EBNA1 cells

21 January 2018

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Gene transfer with synthetic virus-like particles via the integrin-mediated endocytosis pathway

21 January 2018

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Control of culture environment for improved polyethylenimine-mediated transient production of recombinant monoclonal antibodies by CHO cells.

21 January 2018

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Purification of the extracellular domain of the membrane protein GlialCAM expressed in HEK and CHO cells and comparison of the glycosylation

21 January 2018

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Reflections on more than 10 years of TGE approaches

21 January 2018

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Recombinant protein production by transient gene transfer into Mammalian cells

21 January 2018

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Tracking the intracellular path of poly(ethylenimine)/DNA complexes for gene delivery

21 January 2018

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Amniotic fluid cell types and culture

21 January 2018

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Characteristics of a human cell line transformed by DNA from human adenovirus type 5

21 January 2018

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Which mechanism for nuclear import of plasmid DNA complexed with polyethylenimine derivatives?

21 January 2018

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Analysis of expression kinetics and activity of a new B-domain truncated and full-length FVIII protein in three different cell lines

21 January 2018

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Enhanced interferon-beta production by CHO cells through elevated osmolality and reduced culture temperature.

21 January 2018

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Improved green fluorescence

21 January 2018

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Cultivated cells from diagnostic amniocentesis in second trimester pregnancies. I. Clonal morphology and growth potential.

21 January 2018

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Optimizing transient recombinant protein expression in mammalian cells.

21 January 2018

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Optimization of 25 kDa linear polyethylenimine for efficient gene delivery

21 January 2018

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High-level expression of recombinant IgG in the human cell line per.c6.

21 January 2018

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Dilution of reporter gene with stuffer DNA does not alter the transfection efficiency of polyethylenimines

21 January 2018

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Intracellular routing of plasmid DNA during non-viral gene transfer.

21 January 2018

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Rapid, simple and high yield production of recombinant proteins in mammalian cells using a versatile episomal system.

21 January 2018

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Nerve growth factor, brain-derived neurotrophic factor, and neurotrophin-3 levels in human amniotic fluid.

21 January 2018

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Orbital shaker technology for the cultivation of mammalian cells in suspension.

21 January 2018

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Primary metabolism in the new human cell line AGE1.HN at various substrate levels: increased metabolic efficiency and α1-antitrypsin production at reduced pyruvate load

21 January 2018

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Opposing roles of syndecan-1 and syndecan-2 in polyethyleneimine-mediated gene delivery

21 January 2018

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Large-scale transfection of mammalian cells for the fast production of recombinant protein

21 January 2018

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Genetics of somatic mammalian cells. III. Long-term cultivation of euploid cells from human and animal subjects

21 January 2018

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A simple high-yielding process for transient gene expression in CHO cells.

21 January 2018

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A simplified polyethylenimine-mediated transfection process for large-scale and high-throughput applications

21 January 2018

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Serum-free transfection of CHO cells with chemically defined transfection systems and investigation of their potential for transient and stable transfection.

21 January 2018

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Histone modifications are associated with the persistence or silencing of vector-mediated transgene expression in vivo

21 January 2018

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Expression of markers for both neuronal and glial cells in human amniotic epithelial cells.

21 January 2018

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Efficient transformation of primary human amniocytes by E1 functions of Ad5: generation of new cell lines for adenoviral vector production

21 January 2018

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Efficient and reproducible generation of high-expressing, stable human cell lines without need for antibiotic selection

21 January 2018

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Direct regulation of rRNA transcription by fibroblast growth factor 2

21 January 2018

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Episomal maintenance of plasmids with hybrid origins in mouse cells

21 January 2018

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SV40 large T antigen functions in DNA replication and transformation

21 January 2018

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Transient expression of an IL-23R extracellular domain Fc fusion protein in CHO vs. HEK cells results in improved plasma exposure.

21 January 2018

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High-density transient gene expression in suspension-adapted 293 EBNA1 cells

21 January 2018

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Plasmid DNA sequences present in conventional herpes simplex virus amplicon vectors cause rapid transgene silencing by forming inactive chromatin

21 January 2018

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High level expression of functional human IgMs in human PER.C6 cells

21 January 2018

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Non-viral gene therapy: polycation-mediated DNA delivery

21 January 2018

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Cell line specific control of polyethylenimine-mediated transient transfection optimized with "Design of experiments" methodology

21 January 2018

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The amniotic fluid cell proteome.

21 January 2018

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Development of a generic transient transfection process at 100 L scale

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Antibody-based proteomics for human tissue profiling

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Episomal vectors for gene expression in mammalian cells

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Recombinant glycodelin carrying the same type of glycan structures as contraceptive glycodelin-A can be produced in human kidney 293 cells but not in chinese hamster ovary cells

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Mild hypothermia improves transient gene expression yields several fold in Chinese hamster ovary cells

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Valproic acid enhances recombinant mRNA and protein levels in transiently transfected Chinese hamster ovary cells

21 January 2018

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21 January 2018

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High-level protein expression in scalable CHO transient transfection

21 January 2018

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Automation of large scale transient protein expression in mammalian cells

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Intracellular trafficking of nucleic acids

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Identifiers

DOI

10.1002/BIT.24514

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11 September 2017

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