Site-specific, Rep-mediated integration of the intact beta-globin locus in the human erythroleukaemic cell line K562. (Q39979749)

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7 January 2020

title

Site-specific, Rep-mediated integration of the intact beta-globin locus in the human erythroleukaemic cell line K562 (English)

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7 January 2020

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1

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author name string

L Voullaire

2

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H Wardan

3

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R Williamson

4

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J Vadolas

5

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publication date

22 May 2008

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volume

15

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issue

20

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page(s)

1372-1383

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Gene silencing as a threat to the success of gene therapy

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Mapping and direct visualization of a region-specific viral DNA integration site on chromosome 19q13-qter

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Adeno-associated virus (AAV) Rep proteins mediate complex formation between AAV DNA and its integration site in human DNA

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Adeno-associated virus Rep78 protein and terminal repeats enhance integration of DNA sequences into the cellular genome

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Adeno-associated virus Rep proteins target DNA sequences to a unique locus in the human genome

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Targeted integration of adeno-associated virus-derived plasmids in transfected human cells.

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Targeted integration of foreign DNA into a defined locus on chromosome 19 in K562 cells using AAV-derived components

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7 January 2021

A p5 integration efficiency element mediates Rep-dependent integration into AAVS1 at chromosome 19.

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A 16bp Rep binding element is sufficient for mediating Rep-dependent integration into AAVS1.

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Efficient cell proliferation and predominant accumulation of epsilon-globin mRNA in human leukemic K562 cells which produce mostly Hb Gower 1.

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7 January 2021

The transient expression of mRNA coding for Rep protein from AAV facilitates targeted plasmid integration.

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7 January 2021

Development of sensitive fluorescent assays for embryonic and fetal hemoglobin inducers using the human beta -globin locus in erythropoietic cells

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