Identification of Glu-120 as the catalytic nucleophile in Streptomyces lividans endoglucanase celB. (Q41863496)

13 October 2017

Identification of Glu-120 as the catalytic nucleophile in Streptomyces lividans endoglucanase celB. (English)

1 reference

13 October 2017

Streptomyces lividans

object named as

Streptomyces lividans

1 reference

Zechel DL

1

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13 October 2017

He S

series ordinal

2

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13 October 2017

Dupont C

series ordinal

3

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13 October 2017

Withers SG

series ordinal

4

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13 October 2017

language of work or name

English

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publication date

1 November 1998

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13 October 2017

7

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inferred from page(s)

published in

Biochemical Journal

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13 October 2017

336 ( Pt 1)

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13 October 2017

139-145

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The Streptomyces lividans family 12 endoglucanase: construction of the catalytic cre, expression, and X-ray structure at 1.75 A resolution

13 October 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1219852

Mechanism of Bacillus 1,3-1,4-beta-D-glucan 4-glucanohydrolases: kinetics and pH studies with 4-methylumbelliferyl beta-D-glucan oligosaccharides.

5 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1219852

Mechanistic consequences of mutation of active site carboxylates in a retaining beta-1,4-glycanase from Cellulomonas fimi

5 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1219852

Effects of both shortening and lengthening the active site nucleophile of Bacillus circulans xylanase on catalytic activity.

5 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1219852

Structures and mechanisms of glycosyl hydrolases

5 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1219852

Mutational and crystallographic analyses of the active site residues of the Bacillus circulans xylanase

5 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1219852

Purification and characterization of the CelB endoglucanase from Streptomyces lividans 66 and DNA sequence of the encoding gene

5 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1219852

Identification of glutamic acid 78 as the active site nucleophile in Bacillus subtilis xylanase using electrospray tandem mass spectrometry

5 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1219852

Mechanisms of enzymatic glycoside hydrolysis

5 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1219852

Calculation of protein extinction coefficients from amino acid sequence data

5 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1219852

CelS: a novel endoglucanase identified from Erwinia carotovora subsp. carotovora

5 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1219852

Basic local alignment search tool

5 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1219852

Domains in microbial beta-1, 4-glycanases: sequence conservation, function, and enzyme families

5 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1219852

Glu-537, not Glu-461, is the nucleophile in the active site of (lac Z) beta-galactosidase from Escherichia coli

5 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1219852

Ions, ion-pairs and catalysis by thelacZβ-galactosidase ofEscherichia coli

5 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1219852

Mechanism of Agrobacterium beta-glucosidase: kinetic analysis of the role of noncovalent enzyme/substrate interactions.

5 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1219852

Amino acid sequence similarities between low molecular weight endo-1,4-beta-xylanases and family H cellulases revealed by clustering analysis

18 August 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1219852

Stereochemistry, specificity and kinetics of the hydrolysis of reduced cellodextrins by nine cellulases

18 August 2018

1 reference

12 December 2020

Glu280 is the nucleophile in the active site of Clostridium thermocellum CelC, a family A endo-beta-1,4-glucanase

1 reference

12 December 2020

Identification of derivatized peptides without radiolabels: tandem mass spectrometric localization of the tagged active-site nucleophiles of two cellulases and a beta-glucosidase

1 reference

12 December 2020

Identification of the Acid/Base Catalyst in Agrobacterium faecalis .beta.-Glucosidase by Kinetic Analysis of Mutants

1 reference

12 December 2020

Positioning the acid/base catalyst in a glycosidase: studies with Bacillus circulans xylanase

1 reference

12 December 2020

Identification of the Catalytic Nucleophile of Endoglucanase I fromFusarium oxysporumby Mass Spectrometry†

1 reference

12 December 2020

Identification of the active site nucleophile in jack bean alpha-mannosidase using 5-fluoro-beta-L-gulosyl fluoride

1 reference

12 December 2020

The purification and characterization of a beta-glucosidase from Alcaligenes faecalis

1 reference

12 December 2020

Mechanism of Agrobacterium beta-glucosidase: kinetic studies

1 reference

12 December 2020

Inactivation of a beta-glucosidase through the accumulation of a stable 2-deoxy-2-fluoro-alpha-D-glucopyranosyl-enzyme intermediate: a detailed investigation

1 reference

12 December 2020

Identifiers

DOI

10.1042/BJ3360139

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13 October 2017

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13 October 2017

PubMed publication ID

9806895

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