Screening and large-scale expression of membrane proteins in mammalian cells for structural studies (Q41993913)

15 October 2017

Screening and large-scale expression of membrane proteins in mammalian cells for structural studies (English)

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15 October 2017

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7

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15 October 2017

April Goehring

1

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15 October 2017

Chia-Hsueh Lee

2

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15 October 2017

Kevin H Wang

3

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15 October 2017

Jennifer Carlisle Michel

4

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15 October 2017

Derek P Claxton

5

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15 October 2017

Isabelle Baconguis

6

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15 October 2017

Suzanne Fischer

8

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15 October 2017

K Christopher Garcia

9

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15 October 2017

Eric Gouaux

10

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15 October 2017

9 October 2014

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15 October 2017

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15 October 2017

9

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15 October 2017

11

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15 October 2017

2574-2585

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https://scigraph.springernature.com/pub.10.1038/nprot.2014.173

15 October 2017

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X-Ray Structure of Acid-Sensing Ion Channel 1–Snake Toxin Complex Reveals Open State of a Na+-Selective Channel

5 June 2018

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Structure of the TRPV1 ion channel determined by electron cryo-microscopy

5 June 2018

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X-ray structure of dopamine transporter elucidates antidepressant mechanism

5 June 2018

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The structural basis of R-spondin recognition by LGR5 and RNF43

5 June 2018

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Identification of a novel enhancer that binds Sp1 and contributes to induction of cold-inducible RNA-binding protein (cirp) expression in mammalian cells

5 June 2018

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Overexpression of membrane proteins in mammalian cells for structural studies

5 June 2018

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A fluorescence-detection size-exclusion chromatography-based thermostability assay for membrane protein precrystallization screening

5 June 2018

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Structural plasticity and dynamic selectivity of acid-sensing ion channel–spider toxin complexes

5 June 2018

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The mechanism of shared but distinct CSF-1R signaling by the non-homologous cytokines IL-34 and CSF-1

5 June 2018

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Molecular mechanism of ATP binding and ion channel activation in P2X receptors

5 June 2018

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Overexpressing human membrane proteins in stably transfected and clonal human embryonic kidney 293S cells

5 June 2018

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Stabilized G protein binding site in the structure of constitutively active metarhodopsin-II

5 June 2018

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Use of baculovirus BacMam vectors for expression of ABC drug transporters in mammalian cells

5 June 2018

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Principles of activation and permeation in an anion-selective Cys-loop receptor

5 June 2018

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Structural snapshots of full-length Jak1, a transmembrane gp130/IL-6/IL-6Rα cytokine receptor complex, and the receptor-Jak1 holocomplex

5 June 2018

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Maltose-neopentyl glycol (MNG) amphiphiles for solubilization, stabilization and crystallization of membrane proteins

5 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4291175

Evaluation of the Virus Counter® for rapid baculovirus quantitation

5 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4291175

X-ray structure, symmetry and mechanism of an AMPA-subtype glutamate receptor

5 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4291175

A rapid method for titrating baculovirus stocks using the Sf-9 Easy Titer cell line

5 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4291175

Structural basis of receptor sharing by interleukin 17 cytokines

5 June 2018

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Efficient gene delivery into mammalian cells by recombinant baculovirus containing a hybrid cytomegalovirus promoter/Semliki Forest virus replicon

5 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4291175

Pore architecture and ion sites in acid-sensing ion channels and P2X receptors

5 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4291175

An efficient platform for screening expression and crystallization of glycoproteins produced in human cells

5 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4291175

An improved in vitro and in vivo Sindbis virus expression system through host and virus engineering.

5 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4291175

The titerless infected-cells preservation and scale-up (TIPS) method for large-scale production of NO-sensitive human soluble guanylate cyclase (sGC) from insect cells infected with recombinant baculovirus

5 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4291175

Proliferation control strategies to improve productivity and survival during CHO based production culture : A summary of recent methods employed and the effects of proliferation control in product secreting CHO cell lines

5 June 2018

1 reference

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Rapid expression of recombinant proteins in modified CHO cells using the baculovirus system

5 June 2018

1 reference

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BacMam system for high-level expression of recombinant soluble and membrane glycoproteins for structural studies

5 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4291175

Co-evolving stability and conformational homogeneity of the human adenosine A2a receptor

5 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4291175

Valproic acid: a viable alternative to sodium butyrate for enhancing protein expression in mammalian cell cultures

5 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4291175

Conformational thermostabilization of the beta1-adrenergic receptor in a detergent-resistant form

5 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4291175

Cloning and pharmacological characterization of the guinea pig P2X7 receptor orthologue

5 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4291175

Structure of acid-sensing ion channel 1 at 1.9 A resolution and low pH

5 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4291175

Gene delivery by lentivirus vectors

5 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4291175

Implementation of BacMam virus gene delivery technology in a drug discovery setting

5 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4291175

Protein crystallization by surface entropy reduction: optimization of the SER strategy

5 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4291175

Efficient expression of secreted proteases via recombinant BacMam virus

5 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4291175

A time- and cost-efficient system for high-level protein production in mammalian cells

5 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4291175

Fluorescence-detection size-exclusion chromatography for precrystallization screening of integral membrane proteins

5 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4291175

Isolation and analysis of a baculovirus vector that supports recombinant glycoprotein sialylation by SfSWT-1 cells cultured in serum-free medium

5 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4291175

The cold-shock response in cultured mammalian cells: harnessing the response for the improvement of recombinant protein production.

5 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4291175

The application of BacMam technology in nuclear receptor drug discovery

5 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4291175

BacMam recombinant baculoviruses in G protein-coupled receptor drug discovery

5 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4291175

Developing baculovirus-insect cell expression systems for humanized recombinant glycoprotein production

5 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4291175

Titration of KATP channel expression in mammalian cells utilizing recombinant baculovirus transduction

5 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4291175

Structure and function in rhodopsin: high-level expression of rhodopsin with restricted and homogeneous N-glycosylation by a tetracycline-inducible N-acetylglucosaminyltransferase I-negative HEK293S stable mammalian cell line

5 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4291175

Structure and function in rhodopsin: a tetracycline-inducible system in stable mammalian cell lines for high-level expression of opsin mutants

5 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4291175

Quantitation of baculovirus particles by flow cytometry

5 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4291175

Large-scale transient expression in mammalian cells for recombinant protein production

5 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4291175

Efficient transduction of mammalian cells by a recombinant baculovirus having the vesicular stomatitis virus G glycoprotein

5 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4291175

Structure and function in rhodopsin: high level expression of a synthetic bovine opsin gene and its mutants in stable mammalian cell lines

5 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4291175

Baculovirus-mediated gene transfer into mammalian cells

5 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4291175

Sindbis virus DNA-based expression vectors: utility for in vitro and in vivo gene transfer.

5 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4291175

Cloning of an avermectin-sensitive glutamate-gated chloride channel from Caenorhabditis elegans

5 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4291175

Semliki Forest virus expression system: production of conditionally infectious recombinant particles.

5 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4291175

Efficient gene transfer into human hepatocytes by baculovirus vectors

5 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4291175

Recombinant vaccinia viruses as vectors for studying T lymphocyte specificity and function

5 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4291175

A new generation of animal cell expression vectors based on the Semliki Forest virus replicon

5 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4291175

Mild hypothermia improves transient gene expression yields several fold in Chinese hamster ovary cells

5 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4291175

Baculovirus expression system for heterologous multiprotein complexes.

18 August 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4291175

Highly efficient baculovirus-mediated gene transfer into rat chondrocytes.

18 August 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4291175

Investigation of optimal transduction conditions for baculovirus-mediated gene delivery into mammalian cells

18 August 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4291175

Selective elimination of glutamate activation and introduction of fluorescent proteins into a Caenorhabditis elegans chloride channel

18 August 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4291175

Giant nonlinear response from plasmonic metasurfaces coupled to intersubband transitions.

18 August 2018

1 reference

Crossref

https://api.crossref.org/works/10.1038%2FNPROT.2014.173

Proton sensitivity of ASIC1 appeared with the rise of fishes by changes of residues in the region that follows TM1 in the ectodomain of the channel

21 January 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4291175

Some problems of animal virology as studied by the plaque technique

3 November 2018

1 reference

Crossref

https://api.crossref.org/works/10.1038%2FNPROT.2014.173

Update on adenovirus and its vectors

31 March 2020

1 reference

Crossref

https://api.crossref.org/works/10.1038%2FNPROT.2014.173

31 March 2020

Identifiers

DOI

10.1038/NPROT.2014.173

1 reference

15 October 2017

1 reference

15 October 2017

PubMed publication ID

25299155

1 reference