Optimal conditions for the expression of a single-chain antibody (scFv) gene in Pichia pastoris (Q42048325)

15 October 2017

title

Optimal conditions for the expression of a single-chain antibody (scFv) gene in Pichia pastoris (English)

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15 October 2017

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Pichia pastoris

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Xianzong Shi

1

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15 October 2017

Tammy Karkut

2

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15 October 2017

Mahmood Chamankhah

3

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15 October 2017

Michelle Alting-Mees

4

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15 October 2017

Sean M Hemmingsen

5

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15 October 2017

Dwayne Hegedus

6

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15 October 2017

publication date

1 April 2003

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Protein Expression and Purification

15 October 2017

published in

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15 October 2017

volume

28

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15 October 2017

issue

2

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15 October 2017

page(s)

321-330

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Recombinant protein expression in Pichia pastoris

15 October 2017

cites work

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7 January 2021

Recent advances in the expression of foreign genes in Pichia pastoris

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7 January 2021

Fermentation development of recombinant Pichia pastoris expressing the heterologous gene: bovine lysozyme.

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High-level secretion and very efficient isotopic labeling of tick anticoagulant peptide (TAP) expressed in the methylotrophic yeast, Pichia pastoris.

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7 January 2021

The serpins are an expanding superfamily of structurally similar but functionally diverse proteins. Evolution, mechanism of inhibition, novel functions, and a revised nomenclature

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7 January 2021

Phylogeny of the serpin superfamily: implications of patterns of amino acid conservation for structure and function.

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7 January 2021

High level production of soluble single chain antibodies in small-scale Escherichia coli cultures.

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7 January 2021

Production of an Anti-Prostate-Specific Antigen Single-Chain Antibody Fragment from Pichia pastoris

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7 January 2021

Recombinant protein expression in Escherichia coli

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7 January 2021

Escherichia coli skp chaperone coexpression improves solubility and phage display of single-chain antibody fragments.

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7 January 2021

Cosecretion of chaperones and low-molecular-size medium additives increases the yield of recombinant disulfide-bridged proteins

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7 January 2021

Production of a soluble and active MBP-scFv fusion: favorable effect of the leaky tolR strain

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7 January 2021

A novel strategy for production of a highly expressed recombinant protein in an active form

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7 January 2021

Very high expression of an anti-carcinoembryonic antigen single chain Fv antibody fragment in the yeast Pichia pastoris

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7 January 2021

Construction and Expression of Bi-Functional Proteins of Single-Chain Fv With Effector Domains

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7 January 2021

Generation of rabbit monoclonal antibody fragments from a combinatorial phage display library and their production in the yeast Pichia pastoris

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7 January 2021

High-level secretion of two antibody single chain Fv fragments by Pichia pastoris.

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7 January 2021

Cloning and expression in Pichia pastoris of a genetically engineered single chain antibody against the rat transferrin receptor.

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7 January 2021

Production of functionalized single-chain Fv antibody fragments binding to the ED-B domain of the B-isoform of fibronectin in Pichia pastoris

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7 January 2021

Heterologous protein expression in the methylotrophic yeast Pichia pastoris

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7 January 2021

Strategies for optimal synthesis and secretion of heterologous proteins in the methylotrophic yeast Pichia pastoris

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7 January 2021

Expression of human amyloid precursor protein ectodomains in Pichia pastoris: analysis of culture conditions, purification, and characterization.

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7 January 2021

Proteolytic stability of recombinant human serum albumin secreted in the yeast Saccharomyces cerevisiae

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7 January 2021

Low-temperature increases the yield of biologically active herring antifreeze protein in Pichia pastoris

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https://api.crossref.org/works/10.1016%2FS1046-5928%2802%2900706-4

7 January 2021

Human chymotrypsinogen B production with Pichia pastoris by integrated development of fermentation and downstream processing. Part 1. Fermentation.

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https://api.crossref.org/works/10.1016%2FS1046-5928%2802%2900706-4

7 January 2021

Process development for high-level secretory production of carboxypeptidase Y by Saccharomyces cerevisiae

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https://api.crossref.org/works/10.1016%2FS1046-5928%2802%2900706-4

7 January 2021

Asparagine as a nitrogen source for improving the secretion of mouse alpha-amylase in Saccharomyces cerevisiae protease A-deficient strains

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https://api.crossref.org/works/10.1016%2FS1046-5928%2802%2900706-4

7 January 2021

Simple approach to reducing proteolysis during secretory production of human parathyroid hormone in Saccharomyces cerevisiae

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https://api.crossref.org/works/10.1016%2FS1046-5928%2802%2900706-4

7 January 2021

Alcohol oxidase from Hansenula polymorpha CBS 4732

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https://api.crossref.org/works/10.1016%2FS1046-5928%2802%2900706-4

7 January 2021

10.1016/S1046-5928(02)00706-4

Identifiers

DOI

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15 October 2017

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