Tight transcriptional control mechanism ensures stable high-level expression from T7 promoter-based expression plasmids (Q42457004)

31 October 2017

title

Tight transcriptional control mechanism ensures stable high-level expression from T7 promoter-based expression plasmids (English)

1 reference

31 October 2017

Mertens N

1

1 reference

31 October 2017

Remaut E

series ordinal

2

1 reference

31 October 2017

Fiers W

series ordinal

3

1 reference

31 October 2017

publication date

1 February 1995

1 reference

31 October 2017

1 reference

31 October 2017

volume

13

1 reference

31 October 2017

issue

2

1 reference

31 October 2017

page(s)

175-179

1 reference

Construction of Escherichia coli vectors for expression and mutagenesis: synthesis of human c-Myc protein that is initiated at a non-AUG codon in exon 1.

31 October 2017

cites work

1 reference

https://api.crossref.org/works/10.1038%2FNBT0295-175

Bacteriophage T7 RNA polymerase-controlled specific gene expression in Pseudomonas.

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNBT0295-175

A novel expression vector for high-level synthesis and secretion of foreign proteins in Escherichia coli: overproduction of bovine pancreatic phospholipase A2.

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNBT0295-175

The effect of phage T7 lysozyme on the production of biologically active porcine somatotropin in Escherichia coli from a gene transcribed by T7 RNA polymerase.

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNBT0295-175

A two-component T7 system for the overexpression of genes in Pseudomonas aeruginosa

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNBT0295-175

Regulation of coliphage T3 and T7 RNA polymerases by the lac repressor-operator system.

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNBT0295-175

Alterations upstream from the Shine-Dalgarno region and their effect on bacterial gene expression

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNBT0295-175

Protein degradation in E. coli: the lon mutation and bacteriophage lambda N and cII protein stability.

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNBT0295-175

Versatile low-copy-number plasmid vectors for cloning in Escherichia coli.

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNBT0295-175

Tightly regulated tac promoter vectors useful for the expression of unfused and fused proteins in Escherichia coli

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNBT0295-175

Low temperature cultivation of Escherichia coli carrying a rice lipoxygenase L-2 cDNA produces a soluble and active enzyme at a high level

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNBT0295-175

Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNBT0295-175

Vectors for selective expression of cloned DNAs by T7 RNA polymerase

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNBT0295-175

Bacterial bioluminescence: isolation and genetic analysis of functions from Vibrio fischeri

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNBT0295-175

A bacteriophage T7 RNA polymerase/promoter system for controlled exclusive expression of specific genes

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNBT0295-175

7 January 2021

Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes

1 reference

https://api.crossref.org/works/10.1038%2FNBT0295-175

7 January 2021

Expression and refolding of recombinant human fibroblast procathepsin D

1 reference

https://api.crossref.org/works/10.1038%2FNBT0295-175

7 January 2021

Use of bacteriophage T7 lysozyme to improve an inducible T7 expression system

1 reference

https://api.crossref.org/works/10.1038%2FNBT0295-175

7 January 2021

Controlling basal expression in an inducible T7 expression system by blocking the target T7 promoter with lac repressor

1 reference

https://api.crossref.org/works/10.1038%2FNBT0295-175

7 January 2021

Use of T7 RNA polymerase to direct expression of cloned genes

1 reference

https://api.crossref.org/works/10.1038%2FNBT0295-175

7 January 2021

DNA sequence analysis with a modified bacteriophage T7 DNA polymerase

1 reference

https://api.crossref.org/works/10.1038%2FNBT0295-175

7 January 2021

T7 infection-dependent selective expression of cloned genes in P1-lysogenic Escherichia coli.

1 reference

https://api.crossref.org/works/10.1038%2FNBT0295-175

7 January 2021

Promoters largely determine the efficiency of repressor action

1 reference

https://api.crossref.org/works/10.1038%2FNBT0295-175

7 January 2021

Lysis gene expression of RNA phage MS2 depends on a frameshift during translation of the overlapping coat protein gene.

1 reference

https://api.crossref.org/works/10.1038%2FNBT0295-175

7 January 2021

Versatile, multi-featured plasmids for high-level expression of heterologous genes in Escherichia coli: overproduction of human and murine cytokines

1 reference

https://api.crossref.org/works/10.1038%2FNBT0295-175

7 January 2021

Expression of mammalian liver glycolytic/gluconeogenic enzymes in Escherichia coli: recovery of active enzyme is strain and temperature dependent.

1 reference

https://api.crossref.org/works/10.1038%2FNBT0295-175

7 January 2021

Random mutagenesis of the gene for bacteriophage T7 RNA polymerase

1 reference

https://api.crossref.org/works/10.1038%2FNBT0295-175

7 January 2021

Stabilization of T7-promoter-based pARHS expression vectors using the parB locus

1 reference

https://api.crossref.org/works/10.1038%2FNBT0295-175

7 January 2021

Analysis of gene control signals by DNA fusion and cloning in Escherichia coli

1 reference

https://api.crossref.org/works/10.1038%2FNBT0295-175

7 January 2021

Trans-complementable copy-number mutants of plasmid ColE1.

1 reference

https://api.crossref.org/works/10.1038%2FNBT0295-175

7 January 2021

Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid

1 reference

https://api.crossref.org/works/10.1038%2FNBT0295-175

7 January 2021

Inducible high level synthesis of mature human fibroblast interferon inEscherichia coli

1 reference

https://api.crossref.org/works/10.1038%2FNBT0295-175

7 January 2021

Superpolylinkers in cloning and expression vectors

1 reference

https://api.crossref.org/works/10.1038%2FNBT0295-175

7 January 2021

A rapid alkaline extraction procedure for screening recombinant plasmid DNA

1 reference

https://api.crossref.org/works/10.1038%2FNBT0295-175

7 January 2021

Preparative and analytical purification of DNA from agarose

1 reference

https://api.crossref.org/works/10.1038%2FNBT0295-175

7 January 2021

Identifiers

DOI

10.1038/NBT0295-175

1 reference

31 October 2017

1 reference