Production of Streptomyces clavuligerus isopenicillin N synthase in Escherichia coli using two-cistron expression systems (Q42601881)

3 November 2017

Production of Streptomyces clavuligerus isopenicillin N synthase in Escherichia coli using two-cistron expression systems (English)

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?resulttype=core&format=json&query=SRC%3AMED%20AND%20EXT_ID%3A1366605

18 September 2022

1 reference

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18 September 2022

bacterial genes

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?resulttype=core&format=json&query=SRC%3AMED%20AND%20EXT_ID%3A1366605

18 September 2022

1 reference

Streptomyces clavuligerus

object named as

Streptomyces clavuligerus

1 reference

J. L. Doran

1

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?resulttype=core&format=json&query=SRC%3AMED%20AND%20EXT_ID%3A1366605

18 September 2022

B. K. Leskiw

2

1 reference

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18 September 2022

A. K. Petrich

3

1 reference

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18 September 2022

D. W. Westlake

4

1 reference

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18 September 2022

S. E. Jensen

5

1 reference

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18 September 2022

language of work or name

English

1 reference

National Center for Biotechnology Information

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/esummary.fcgi?db=pubmed&retmode=json&id=1366605

18 September 2022

publication date

1 June 1990

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Journal of Industrial Microbiology & Biotechnology

3 November 2017

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3 November 2017

5

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3 November 2017

4

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3 November 2017

197-206

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Isolation, sequence determination and expression in Escherichia coli of the isopenicillin N synthetase gene from Cephalosporium acremonium

3 November 2017

1 reference

12 December 2020

Analysis of the role of cysteine residues in isopenicillin N synthetase activity by site-directed mutagenesis

1 reference

12 December 2020

Electrophoretic analysis of the major polypeptides of the human erythrocyte membrane

1 reference

12 December 2020

Purification and characterization of cloned isopenicillin N synthetase.

1 reference

12 December 2020

Systems for polyacrylamide gel electrophoresis

1 reference

12 December 2020

Activity of a Streptomyces transcriptional terminator in Escherichia coli

1 reference

12 December 2020

Specific-purpose plasmid cloning vectors II. Broad host range, high copy number, RSF 1010-derived vectors, and a host-vector system for gene cloning in Pseudomonas

1 reference

12 December 2020

Optimization of gene expression in Streptomyces lividans by a transcription terminator

1 reference

12 December 2020

Expression and secretion of foreign proteins in Escherichia coli

1 reference

12 December 2020

Cloning and expression of the isopenicillin N synthetase gene from Penicillium chrysogenum

1 reference

12 December 2020

Analysis of gene control signals by DNA fusion and cloning in Escherichia coli

1 reference

12 December 2020

Cyclization of delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine to penicillins by cell-free extracts of Streptomyces clavuligerus

1 reference

12 December 2020

Cloning and comparative sequence analysis of the gene coding for isopenicillin N synthase in Streptomyces

1 reference

12 December 2020

Cloning and expression in Escherichia coli of isopenicillin N synthetase genes from Streptomyces lipmanii and Aspergillus nidulans

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12 December 2020

RNA polymerase heterogeneity in Streptomyces coelicolor

1 reference

12 December 2020

N-Methyl transferase of Streptomyces erythraeus that confers resistance to the macrolide-lincosamide-streptogramin B antibiotics: amino acid sequence and its homology to cognate R-factor enzymes from pathogenic bacilli and cocci.

1 reference

12 December 2020

The use of thin acrylamide gels for DNA sequencing

1 reference

12 December 2020

Filter replicas and permanent collections of recombinant DNA plasmids

1 reference

12 December 2020

Cloning and nucleotide sequence determination of the isopenicillin N synthetase gene from Streptomyces clavuligerus

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12 December 2020

The relationship between base composition and codon usage in bacterial genes and its use for the simple and reliable identification of protein-coding sequences

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12 December 2020

DNA sequencing with chain-terminating inhibitors

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12 December 2020

The making of strand-specific M13 probes

1 reference

12 December 2020

The use of NaOH as transfer solution of DNA onto nylon membrane decreases the hybridization efficiency

1 reference

12 December 2020

Posttranscriptional regulatory mechanisms in Escherichia coli

1 reference

12 December 2020

A simple method for DNA restriction site mapping

1 reference

12 December 2020

High performance liquid chromatographic assay of cyclization activity in cell-free systems from Streptomyces clavuligerus

1 reference

12 December 2020

Translation of a synthetic two-cistron mRNA in Escherichia coli

1 reference

12 December 2020

Optimal computer folding of large RNA sequences using thermodynamics and auxiliary information

1 reference

12 December 2020

Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors

1 reference

12 December 2020

Purification of isopenicillin N synthetase from Streptomyces clavuligerus

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12 December 2020

Production of single-stranded plasmid DNA

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12 December 2020

A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding

1 reference

12 December 2020

Cloning and characterization of the isopenicillin N synthetase gene mediating the formation of the beta-lactam ring in Aspergillus nidulans.

1 reference

12 December 2020

Enzymatic approach to syntheses of unnatural beta-lactams

1 reference

12 December 2020

21 Transformation and preservation of competent bacterial cells by freezing

1 reference

12 December 2020

Inefficient translation initiation causes premature transcription termination in the lacZ gene

1 reference

12 December 2020

The biosynthesis of penicillins and cephalosporins

1 reference

12 December 2020

Gene expression in Streptomyces: Construction and application of promoter-probe plasmid vectors in Streptomyces lividans

1 reference

12 December 2020

At least three different RNA polymerase holoenzymes direct transcription of the agarase gene (dagA) of Streptomyces coelicolor A3(2)

1 reference

12 December 2020

44 Prokaryotic coupled transcription — translation

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12 December 2020

Identifiers

DOI

10.1007/BF01569677

1 reference

3 November 2017

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