Covalent chromatography. Preparation of fully active papain from dried papaya latex (Q42923715)

11 November 2017

Covalent chromatography. Preparation of fully active papain from dried papaya latex (English)

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26 February 2023

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26 February 2023

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26 February 2023

author name string

K. Brocklehurst

1

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26 February 2023

J. Carlsson

2

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26 February 2023

M. P. Kierstan

3

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26 February 2023

E. M. Crook

4

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26 February 2023

language of work or name

English

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publication date

1 July 1973

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PMCID

1177736

full work available at URL

11 November 2017

https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/4733241/?tool=EBI

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https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/4733241/pdf/?tool=EBI

26 February 2023

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open access

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https://europepmc.org/articles/PMC1177736

26 February 2023

1 reference

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https://europepmc.org/articles/PMC1177736?pdf=render

26 February 2023

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https://portlandpress.com/biochemj/article-pdf/133/3/573/561015/bj1330573.pdf

26 February 2023

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Portable Document Format

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26 February 2023

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11 November 2017

133

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11 November 2017

3

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11 November 2017

573-584

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Computer Programmes for Processing Enzyme Kinetic Data

11 November 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1177736

The Determination of the Concentration of Hydrolytic Enzyme Solutions: α-Chymotrypsin, Trypsin, Papain, Elastase, Subtilisin, and Acetylcholinesterase1

27 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1177736

Protein purification by affinity chromatography. Derivatizations of agarose and polyacrylamide beads

27 May 2018

1 reference

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27 May 2018

1 reference

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Reactivities of the various protonic states in the reactions of papain and of L-cysteine with 2,2'- and with 4,4'- dipyridyl disulphide: evidence for nucleophilic reactivity in the un-ionized thiol group of the cysteine-25 residue of papain occasion

27 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1177736

The activation reaction of papain.

27 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1177736

Reactions of papain and of low-molecular-weight thiols with some aromatic disulphides. 2,2'-Dipyridyl disulphide as a convenient active-site titrant for papain even in the presence of other thiols

27 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1177736

The reaction of papain with Ellman's reagent (5,5'-dithiobis- (2-nitrobenzoate) dianion)

27 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1177736

Isolation by Covalent Affinity Chromatography of the Penicillin-Binding Components from Membranes of Bacillus subtilis

27 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1177736

Staining of protein zones after isoelectric focusing in polyacrylamide gels.

27 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1177736

The Purification of Papain by Affinity Chromatography

27 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1177736

The mutability of stem bromelain: evidence for perturbation by structural transitions of the parameters that characterize the reaction of the essential thiol group of bromelain with 2,2'-dipyridyl disulphide

16 August 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1177736

A novel reactivity of papain and a convenient active site titration in the presence of other thiols

16 August 2018

1 reference

12 December 2020

The mechanism of the activation of papain

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12 December 2020

Kinetics of papain action. III. Hydrolysis of benzoyl-L-arginine ethyl ester

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12 December 2020

Chemical Coupling of Proteins to Agarose

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12 December 2020

KINETICS OF PAPAIN-CATALYZED HYDROLYSIS OF ALPHA-N-BENZOYL-L-ARGININE ETHYL ESTER AND ALPHA-N-BENZOYL-L-ARGININAMIDE

1 reference

12 December 2020

THE SULFUR DISTRIBUTION OF PAPAIN

1 reference

12 December 2020

An agarose mercurial column for the separation of mercaptopapain and nonmercaptopapain

1 reference

12 December 2020

Crystalline papain. I. Preparation, specificity, and activation

1 reference

12 December 2020

Chemical Fixation of Enzymes to Cyanogen Halide Activated Polysaccharide Carriers

1 reference

12 December 2020

Chemical Coupling of Peptides and Proteins to Polysaccharides by Means of Cyanogen Halides

1 reference

12 December 2020

Determination of sulfhydryl groups with 2,2′- or 4,4′-dithiodipyridine

1 reference

12 December 2020

A covalent affinity column for the purification of acetylcholinesterase

1 reference

12 December 2020

The rate-limiting reaction in papain action as derived from the reaction of the enzyme with chloroacetic acid

1 reference

12 December 2020

Identifiers

DOI

10.1042/BJ1330573

1 reference

release_cw53xqpv7vdv5azeymguimpfai

11 November 2017

1 reference

Fatcat

https://api.fatcat.wiki/v0/release/cw53xqpv7vdv5azeymguimpfai

24 November 2022

mapped directly with Wikidata item

PMCID

1177736

1 reference

11 November 2017

1 reference

PMCID

1177736