A practical summary of site-specific recombination, conditional mutagenesis, and tamoxifen induction of CreERT2. (Q42941347)

11 November 2017

A practical summary of site-specific recombination, conditional mutagenesis, and tamoxifen induction of CreERT2. (English)

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Konstantinos Anastassiadis

11 November 2017

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1

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11 November 2017

Stefan Glaser

2

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11 November 2017

Andrea Kranz

3

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11 November 2017

Kaj Berhardt

4

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11 November 2017

A Francis Stewart

5

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11 November 2017

language of work or name

English

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publication date

1 January 2010

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11 November 2017

Methods in Enzymology

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11 November 2017

477

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11 November 2017

109-123

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Site-specific integration of DNA into wild-type and mutant lox sites placed in the plant genome.

11 November 2017

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2810%2977007-5

7 January 2021

Dre recombinase, like Cre, is a highly efficient site-specific recombinase in E. coli, mammalian cells and mice

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2810%2977007-5

7 January 2021

Cryptic boundaries in roof plate and choroid plexus identified by intersectional gene activation

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2810%2977007-5

7 January 2021

New mouse lines for the analysis of neuronal morphology using CreER(T)/loxP-directed sparse labeling

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2810%2977007-5

7 January 2021

Coping with kinetic and thermodynamic barriers: RMCE, an efficient strategy for the targeted integration of transgenes

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2810%2977007-5

7 January 2021

Talking about a Revolution

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https://api.crossref.org/works/10.1016%2FS0076-6879%2810%2977007-5

7 January 2021

Different thermostabilities of FLP and Cre recombinases: implications for applied site-specific recombination

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2810%2977007-5

7 January 2021

Improved properties of FLP recombinase evolved by cycling mutagenesis.

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2810%2977007-5

7 January 2021

ER-based double iCre fusion protein allows partial recombination in forebrain

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2810%2977007-5

7 January 2021

New insight into site-specific recombination from Flp recombinase-DNA structures

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2810%2977007-5

7 January 2021

Recombineering: a powerful new tool for mouse functional genomics

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2810%2977007-5

7 January 2021

Modification of gene activity in mouse embryos in utero by a tamoxifen-inducible form of Cre recombinase

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2810%2977007-5

7 January 2021

Crystal structure of a wild-type Cre recombinase-loxP synapse reveals a novel spacer conformation suggesting an alternative mechanism for DNA cleavage activation

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2810%2977007-5

7 January 2021

Ligand-activated site-specific recombination in mice

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2810%2977007-5

7 January 2021

Regulation of Cre recombinase activity by mutated estrogen receptor ligand-binding domains

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2810%2977007-5

7 January 2021

Synapsis of loxP sites by Cre recombinase

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2810%2977007-5

7 January 2021

Current issues in mouse genome engineering

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2810%2977007-5

7 January 2021

The histone 3 lysine 4 methyltransferase, Mll2, is only required briefly in development and spermatogenesis

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2810%2977007-5

7 January 2021

Mechanisms of site-specific recombination.

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2810%2977007-5

7 January 2021

Toxicity of ligand-dependent Cre recombinases and generation of a conditional Cre deleter mouse allowing mosaic recombination in peripheral tissues.

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2810%2977007-5

7 January 2021

Engineering chromosomes in mice through targeted meiotic recombination (TAMERE)

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2810%2977007-5

7 January 2021

Direct hematological toxicity and illegitimate chromosomal recombination caused by the systemic activation of CreERT2.

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2810%2977007-5

7 January 2021

The role of the loxP spacer region in P1 site-specific recombination

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2810%2977007-5

7 January 2021

Myf-5 revisited: loss of early myotome formation does not lead to a rib phenotype in homozygous Myf-5 mutant mice

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2810%2977007-5

7 January 2021

Regulation of Cre recombinase activity by the synthetic steroid RU 486.

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2810%2977007-5

7 January 2021

Genetic fate-mapping approaches: new means to explore the embryonic origins of the cochlear nucleus

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2810%2977007-5

7 January 2021

Selection-marker-free modification of the murine beta-casein gene using a lox2272 [correction of lox2722] site

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2810%2977007-5

7 January 2021

An improved Flp deleter mouse in C57Bl/6 based on Flpo recombinase

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2810%2977007-5

7 January 2021

Inducible gene targeting in mice

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2810%2977007-5

7 January 2021

Role of nucleotide sequences of loxP spacer region in Cre-mediated recombination

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2810%2977007-5

7 January 2021

Ligand-regulated site-specific recombination

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2810%2977007-5

7 January 2021

Growth inhibition and DNA damage induced by Cre recombinase in mammalian cells

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2810%2977007-5

7 January 2021

Rapid modification of bacterial artificial chromosomes by ET-recombination.

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2810%2977007-5

7 January 2021

Dissecting the role of N-myc in development using a single targeting vector to generate a series of alleles.

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2810%2977007-5

7 January 2021

Cre activity causes widespread apoptosis and lethal anemia during embryonic development.

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2810%2977007-5

7 January 2021

Agouti C57BL/6N embryonic stem cells for mouse genetic resources

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2810%2977007-5

7 January 2021

Conditional gene targeting

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https://api.crossref.org/works/10.1016%2FS0076-6879%2810%2977007-5

7 January 2021

High-efficiency FLP and PhiC31 site-specific recombination in mammalian cells.

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2810%2977007-5

7 January 2021

Comparative kinetic analysis of FLP and cre recombinases: mathematical models for DNA binding and recombination.

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2810%2977007-5

7 January 2021

Quantitative comparison of DNA looping in vitro and in vivo: chromatin increases effective DNA flexibility at short distances

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2810%2977007-5

7 January 2021

High-efficiency deleter mice show that FLPe is an alternative to Cre-loxP

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2810%2977007-5

7 January 2021

DNA recombination with a heterospecific Cre homolog identified from comparison of the pac-c1 regions of P1-related phages

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2810%2977007-5

7 January 2021

Efficient FLP recombination in mouse ES cells and oocytes.

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2810%2977007-5

7 January 2021

Use of mutated FLP recognition target (FRT) sites for the exchange of expression cassettes at defined chromosomal loci

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2810%2977007-5

7 January 2021

Illegitimate Cre-dependent chromosome rearrangements in transgenic mouse spermatids

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2810%2977007-5

7 January 2021

Vagaries of conditional gene targeting

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https://api.crossref.org/works/10.1016%2FS0076-6879%2810%2977007-5

7 January 2021

A directional strategy for monitoring Cre-mediated recombination at the cellular level in the mouse

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2810%2977007-5

7 January 2021

Genomewide production of multipurpose alleles for the functional analysis of the mouse genome

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2810%2977007-5

7 January 2021

Engineering embryonic stem cells with recombinase systems

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2810%2977007-5

7 January 2021

Stringent doxycycline dependent control of CRE recombinase in vivo

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2810%2977007-5

7 January 2021

Temporally and spatially regulated somatic mutagenesis in mice

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2810%2977007-5

7 January 2021

Rapid generation of inducible mouse mutants

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2810%2977007-5

7 January 2021

DNA recognition by the FLP recombinase of the yeast 2 mu plasmid. A mutational analysis of the FLP binding site

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2810%2977007-5

7 January 2021

Codon-improved Cre recombinase (iCre) expression in the mouse

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2810%2977007-5

7 January 2021

Diversity in the serine recombinases

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2810%2977007-5

7 January 2021

Engineering the mouse genome with bacterial artificial chromosomes to create multipurpose alleles.

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2810%2977007-5

7 January 2021

A reliable lacZ expression reporter cassette for multipurpose, knockout-first alleles.

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2810%2977007-5

7 January 2021

Mutational analysis of loxP sites for efficient Cre-mediated insertion into genomic DNA.

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2810%2977007-5

7 January 2021

Chromosome engineering in ES cells

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2810%2977007-5

7 January 2021

A structural view of cre-loxp site-specific recombination

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2810%2977007-5

7 January 2021

Manipulating the mouse genome to engineer precise functional syntenic replacements with human sequence.

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2810%2977007-5

7 January 2021

Toward simpler and faster genome-wide mutagenesis in mice.

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2810%2977007-5

7 January 2021

High-efficient FLPo deleter mice in C57BL/6J background

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2810%2977007-5

7 January 2021

Engineering chromosomal rearrangements in mice

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2810%2977007-5

7 January 2021

A new logic for DNA engineering using recombination in Escherichia coli

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2810%2977007-5

7 January 2021

10.1016/S0076-6879(10)77007-5

Identifiers

DOI

1 reference

11 November 2017

1 reference