Directed evolution of restriction endonuclease BstYI to achieve increased substrate specificity (Q43030612)

12 November 2017

Directed evolution of restriction endonuclease BstYI to achieve increased substrate specificity (English)

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12 November 2017

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James C Samuelson

1

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PubMed ID

12054862

12 November 2017

Shuang-yong Xu

series ordinal

2

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PubMed ID

12054862

12 November 2017

language of work or name

English

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publication date

1 June 2002

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Journal of Molecular Biology

12 November 2017

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12 November 2017

319

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12 November 2017

3

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12 November 2017

673-683

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Genetic analysis of the base-specific contacts of BamHI restriction endonuclease

12 November 2017

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2802%2900343-1

A mutant of BamHI restriction endonuclease which requires N6-methyladenine for cleavage

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2802%2900343-1

Mutational analysis of the function of Met137 and Ile197, two amino acids implicated in sequence-specific DNA recognition by the EcoRI endonuclease.

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2802%2900343-1

Protein engineering of the restriction endonuclease EcoRV . Structure-guided design of enzyme variants that recognize the base pairs flanking the recognition site

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2802%2900343-1

Temperature-sensitive mutants of the EcoRI endonuclease

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2802%2900343-1

Saturation mutagenesis of His114 of EcoRI reveals relaxed-specificity mutants

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2802%2900343-1

Substrate recognition by the EcoRI endonuclease.

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2802%2900343-1

Changing the hydrogen-bonding potential in the DNA binding site of EcoRI by site-directed mutagenesis drastically reduces the enzymatic activity, not, however, the preference of this restriction endonuclease for cleavage within the site-GAATTC-.

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2802%2900343-1

Recognition of flanking DNA sequences by EcoRV endonuclease involves alternative patterns of water-mediated contacts

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2802%2900343-1

On the possibilities and limitations of rational protein design to expand the specificity of restriction enzymes: a case study employing EcoRV as the target

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2802%2900343-1

Towards the design of rare cutting restriction endonucleases: using directed evolution to generate variants of EcoRV differing in their substrate specificity by two orders of magnitude

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2802%2900343-1

Refinement of Eco RI endonuclease crystal structure: a revised protein chain tracing

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2802%2900343-1

How the EcoRI endonuclease recognizes and cleaves DNA.

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2802%2900343-1

Direct selection of binding proficient/catalytic deficient variants of BamHI endonuclease

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2802%2900343-1

Cloning and characterization of the Bg/II restriction-modification system reveals a possible evolutionary footprint

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2802%2900343-1

The 'endo-blue method' for direct cloning of restriction endonuclease genes in E. coli.

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2802%2900343-1

SOS induction as an in vivo assay of enzyme-DNA interactions

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2802%2900343-1

Inducible DNA repair systems

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2802%2900343-1

Effects of surrounding sequence on the suppression of nonsense codons

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2802%2900343-1

Structure and function of type II restriction endonucleases

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2802%2900343-1

Understanding the immutability of restriction enzymes: crystal structure of BglII and its DNA substrate at 1.5 A resolution

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2802%2900343-1

Structure of Bam HI endonuclease bound to DNA: partial folding and unfolding on DNA binding

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2802%2900343-1

Crosslinking the EcoRV restriction endonuclease across the DNA-binding site reveals transient intermediates and conformational changes of the enzyme during DNA binding and catalytic turnover

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2802%2900343-1

Conformational transitions and structural deformability of EcoRV endonuclease revealed by crystallographic analysis

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2802%2900343-1

Role of protein-induced bending in the specificity of DNA recognition: crystal structure of EcoRV endonuclease complexed with d(AAAGAT) + d(ATCTT)

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2802%2900343-1

Randomization of genes by PCR mutagenesis

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2802%2900343-1

inferred from DOI database lookup

7 January 2021

based on heuristic

Generation of large libraries of random mutants in Bacillus subtilis by PCR-based plasmid multimerization

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2802%2900343-1

inferred from DOI database lookup

7 January 2021

based on heuristic

Identifiers

DOI

10.1016/S0022-2836(02)00343-1

1 reference

12 November 2017

1 reference

PubMed ID

12054862