Identification of reference genes for RT-qPCR expression analysis in Arabidopsis and tomato seeds (Q43490004)

24 November 2017

title

Identification of reference genes for RT-qPCR expression analysis in Arabidopsis and tomato seeds (English)

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24 November 2017

3

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24 November 2017

Leónie Bentsink

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Wilco Ligterink

object named as

Wilco Ligterink

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author name string

Bas J W Dekkers

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24 November 2017

Leo Willems

2

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24 November 2017

R P Marieke van Bolderen-Veldkamp

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Henk W M Hilhorst

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publication date

18 August 2011

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Plant and Cell Physiology

24 November 2017

number of pages

10

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based on heuristic

inferred from page(s)

published in

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24 November 2017

volume

53

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24 November 2017

issue

1

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24 November 2017

page(s)

28-37

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Normalization of real-time quantitative reverse transcription-PCR data: a model-based variance estimation approach to identify genes suited for normalization, applied to bladder and colon cancer data sets

24 November 2017

cites work

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Elucidating the germination transcriptional program using small molecules

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Cloning of DOG1, a quantitative trait locus controlling seed dormancy in Arabidopsis.

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Natural variation for seed dormancy in Arabidopsis is regulated by additive genetic and molecular pathways

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The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments

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Gene expression profiling reveals defined functions of the ATP-binding cassette transporter COMATOSE late in phase II of germination.

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Genome-wide identification and testing of superior reference genes for transcript normalization in Arabidopsis

21 January 2018

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Interaction between sugar and abscisic acid signalling during early seedling development in Arabidopsis

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Human housekeeping genes are compact

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Selection of internal control genes for quantitative real-time RT-PCR studies during tomato development process

21 January 2018

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Seed dormancy release in Arabidopsis Cvi by dry after-ripening, low temperature, nitrate and light shows common quantitative patterns of gene expression directed by environmentally specific sensing.

21 January 2018

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A guideline to family-wide comparative state-of-the-art quantitative RT-PCR analysis exemplified with a Brassicaceae cross-species seed germination case study

21 January 2018

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Normalization of qRT-PCR data: the necessity of adopting a systematic, experimental conditions-specific, validation of references

21 January 2018

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The lack of a systematic validation of reference genes: a serious pitfall undervalued in reverse transcription-polymerase chain reaction (RT-PCR) analysis in plants.

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qBase relative quantification framework and software for management and automated analysis of real-time quantitative PCR data

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RefGenes: identification of reliable and condition specific reference genes for RT-qPCR data normalization

21 January 2018

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Real-time RT-PCR normalisation; strategies and considerations.

21 January 2018

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Ethylene interacts with abscisic acid to regulate endosperm rupture during germination: a comparative approach using Lepidium sativum and Arabidopsis thaliana

21 January 2018

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Reference gene selection for quantitative real-time PCR normalization in tomato subjected to nitrogen, cold, and light stress.

21 January 2018

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Selection and reliability of internal reference genes for quantitative PCR verification of transcriptomics during the differentiation process of porcine adult mesenchymal stem cells.

21 January 2018

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Genome-wide profiling of stored mRNA in Arabidopsis thaliana seed germination: epigenetic and genetic regulation of transcription in seed

21 January 2018

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Defining reference genes in Oryza sativa using organ, development, biotic and abiotic transcriptome datasets

21 January 2018

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Quantification of mRNA using real-time RT-PCR

21 January 2018

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A germination-specific endo-beta-mannanase gene is expressed in the micropylar endosperm cap of tomato seeds

21 January 2018

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Gibberellin biosynthesis and response during Arabidopsis seed germination

21 January 2018

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Arabidopsis ABA INSENSITIVE4 regulates lipid mobilization in the embryo and reveals repression of seed germination by the endosperm.

21 January 2018

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Determination of stable housekeeping genes, differentially regulated target genes and sample integrity: BestKeeper – Excel-based tool using pair-wise correlations

21 January 2018

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Assumption-free analysis of quantitative real-time polymerase chain reaction (PCR) data

21 January 2018

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Amplification efficiency: linking baseline and bias in the analysis of quantitative PCR data

21 January 2018

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A gene expression map of Arabidopsis thaliana development

21 January 2018

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The conserved splicing factor SUA controls alternative splicing of the developmental regulator ABI3 in Arabidopsis.

21 January 2018

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Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes

21 January 2018

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An "Electronic Fluorescent Pictograph" browser for exploring and analyzing large-scale biological data sets

21 January 2018

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Activation of gibberellin biosynthesis and response pathways by low temperature during imbibition of Arabidopsis thaliana seeds

21 January 2018

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21 January 2018

Identifiers

DOI

10.1093/PCP/PCR113

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24 November 2017

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