Expression, purification, crystallization, and preliminary X-ray analysis of recombinant human saposin B. (Q44268108)

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16 December 2019

title

Expression, purification, crystallization, and preliminary X-ray analysis of recombinant human saposin B (English)

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16 December 2019

6

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16 December 2019

3

Julian P Whitelegge

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16 December 2019

author name string

Victoria E Ahn

1

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16 December 2019

Kym F Faull

2

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16 December 2019

Jason Higginson

4

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16 December 2019

Arvan L Fluharty

5

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16 December 2019

language of work or name

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1 January 2003

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Protein Expression and Purification

16 December 2019

published in

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16 December 2019

volume

27

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16 December 2019

issue

1

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16 December 2019

page(s)

186-193

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Activator proteins and topology of lysosomal sphingolipid catabolism.

16 December 2019

cites work

1 reference

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7 January 2021

Topology of glycosphingolipid degradation.

1 reference

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7 January 2021

Degradation of membrane-bound ganglioside GM1. Stimulation by bis(monoacylglycero)phosphate and the activator proteins SAP-B and GM2-AP.

1 reference

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7 January 2021

Glycosphingolipid specificity of the human sulfatide activator protein

1 reference

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7 January 2021

Comparative lipid binding study on the cerebroside sulfate activator (saposin B).

1 reference

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7 January 2021

Preparation of the cerebroside sulfate activator (CSAct or saposin B) from human urine

1 reference

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7 January 2021

Disulfide connectivity in cerebroside sulfate activator is not necessary for biological activity or alpha-helical content but is necessary for trypsin resistance and strong ligand binding

1 reference

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7 January 2021

The cerebroside sulfate activator from pig kidney: purification and molecular structure.

1 reference

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7 January 2021

The cerebroside sulfate activator from pig kidney: derivitization, cerebroside sulfate binding, and metabolic correction

1 reference

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7 January 2021

Porcine cerebroside sulfate activator: further structural characterization and disulfide identification

1 reference

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7 January 2021

Structural analysis of saposin C and B. Complete localization of disulfide bridges

1 reference

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7 January 2021

Porcine cerebroside sulfate activator (saposin B) secondary structure: CD, FTIR, and NMR studies.

1 reference

https://api.crossref.org/works/10.1016%2FS1046-5928%2802%2900597-1

7 January 2021

Hydrogen-deuterium exchange signature of porcine cerebroside sulfate activator protein.

1 reference

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7 January 2021

Characterization of a mutation in a family with saposin B deficiency: a glycosylation site defect

1 reference

https://api.crossref.org/works/10.1016%2FS1046-5928%2802%2900597-1

7 January 2021

The effect of carbohydrate removal on stability and activity of saposin B.

1 reference

https://api.crossref.org/works/10.1016%2FS1046-5928%2802%2900597-1

7 January 2021

Structure of the asparagine-linked sugar chains of porcine kidney and human urine cerebroside sulfate activator protein

1 reference

https://api.crossref.org/works/10.1016%2FS1046-5928%2802%2900597-1

7 January 2021

Saposins and their interaction with lipids

1 reference

https://api.crossref.org/works/10.1016%2FS1046-5928%2802%2900597-1

7 January 2021

The amoebapore superfamily

1 reference

https://api.crossref.org/works/10.1016%2FS1046-5928%2802%2900597-1

7 January 2021

Specific recognition of N-acetylneuraminic acid in the GM2 epitope by human GM2 activator protein

1 reference

https://api.crossref.org/works/10.1016%2FS1046-5928%2802%2900597-1

7 January 2021

Preparation of selenomethionyl proteins for phase determination

1 reference

https://api.crossref.org/works/10.1016%2FS1046-5928%2802%2900597-1

7 January 2021

A conserved disulfide motif in human tear lipocalins influences ligand binding

1 reference

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7 January 2021

MacProMass: a computer program to correlate mass spectral data to peptide and protein structures

1 reference

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7 January 2021

Oxidation of selenomethionine: some MADness in the method!

1 reference

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7 January 2021

The CCP4 suite: programs for protein crystallography

1 reference

https://api.crossref.org/works/10.1016%2FS1046-5928%2802%2900597-1

7 January 2021

Mutations that allow disulfide bond formation in the cytoplasm of Escherichia coli.

1 reference

https://api.crossref.org/works/10.1016%2FS1046-5928%2802%2900597-1

7 January 2021

Methionine oxidation within the cerebroside-sulfate activator protein (CSAct or Saposin B).

1 reference

https://api.crossref.org/works/10.1016%2FS1046-5928%2802%2900597-1

7 January 2021

Solvent content of protein crystals

1 reference

https://api.crossref.org/works/10.1016%2FS1046-5928%2802%2900597-1

7 January 2021

Analysis and characterization of data from twinned crystals.

1 reference

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7 January 2021

[22] Detecting and overcoming crystal twinning

1 reference

https://api.crossref.org/works/10.1016%2FS1046-5928%2802%2900597-1

7 January 2021

Improved R-factors for diffraction data analysis in macromolecular crystallography

1 reference

https://api.crossref.org/works/10.1016%2FS1046-5928%2802%2900597-1

7 January 2021

10.1016/S1046-5928(02)00597-1

Identifiers

DOI

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https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:12510003%20AND%20SRC:MED&resulttype=core&format=json

16 December 2019

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