Higher random oligo concentration improves reverse transcription yield of cDNA from bioptic tissues and quantitative RT-PCR reliability. (Q44786478)

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2 February 2020

title

Higher random oligo concentration improves reverse transcription yield of cDNA from bioptic tissues and quantitative RT-PCR reliability (English)

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2 February 2020

3

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2 February 2020

Giorgio Stanta

5

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2 February 2020

author name string

Ermanno Nardon

1

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2 February 2020

Marisa Donada

2

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2 February 2020

Isabella Dotti

4

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publication date

18 July 2009

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Experimental and Molecular Pathology

2 February 2020

published in

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volume

87

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issue

2

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2 February 2020

page(s)

146-151

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Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays

2 February 2020

cites work

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High temperature cDNA synthesis by AMV reverse transcriptase improves the specificity of PCR.

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Quantitative mRNA expression analysis from formalin-fixed, paraffin-embedded tissues using 5' nuclease quantitative reverse transcription-polymerase chain reaction

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Variance in the expression of 5-Fluorouracil pathway genes in colorectal cancer

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Sensitivity and accuracy of quantitative real-time polymerase chain reaction using SYBR green I depends on cDNA synthesis conditions

7 January 2021

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Quantification of mRNA using real-time RT-PCR

7 January 2021

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Theoretical uncertainty of measurements using quantitative polymerase chain reaction.

7 January 2021

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Product differentiation by analysis of DNA melting curves during the polymerase chain reaction

7 January 2021

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Quantitative gene expression analysis in microdissected archival formalin-fixed and paraffin-embedded tumor tissue

7 January 2021

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Properties of the reverse transcription reaction in mRNA quantification

7 January 2021

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Comparison of reverse transcriptases in gene expression analysis.

7 January 2021

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Reverse transcription using random pentadecamer primers increases yield and quality of resulting cDNA.

7 January 2021

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PCR inhibition by reverse transcriptase leads to an overestimation of amplification efficiency

7 January 2021

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Differential priming of RNA templates during cDNA synthesis markedly affects both accuracy and reproducibility of quantitative competitive reverse-transcriptase PCR.

7 January 2021

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7 January 2021

Identifiers

DOI

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2 February 2020

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